Development of Rapid Assessment Method to Determine Bacterial Viability Based on Ultraviolet and Visible (UV-Vis) Spectroscopy Analysis Including Application to Bioaerosols

Park, Chul Woo; Yoon, Ki Hyun; Byeon, Jeong Hoon; Kim, Kyoungsik; Hwang, Jungho

doi:10.4209/aaqr.2011.08.0129

Cited by 29 publications

(19 citation statements)

References 42 publications

(35 reference statements)

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“…Bioaerosols in the atmosphere pose potential health hazards such as allergy, asthma, bronchitis, dermatitis and diarrhea (Lavoie et al, 2006;Taha et al, 2006;Park et al, 2012). Deleterious health effects are possible even when the bioaerosols are non-viable (Griffiths and DeCosemo, 1994).…”

Section: Introductionmentioning

confidence: 99%

Passive Release of Fungal Spores from Synthetic Solid Waste Surfaces

Gopalakrishnan

Devassikutty

Mathew

et al. 2016

Aerosol Air Qual. Res.

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Passive release of fungal spores can occur from various natural and anthropogenic sources leading to significant concentrations in ambient air with potential effect on health and climate. The estimation of fungal spore release is a critical parameter necessary for the realistic assessment of health risk using dispersion models and in global climate modeling. This paper presents results from experiments conducted to seek a better understanding of the process of passive fungal spore due to wind. Laboratory studies were conducted to measure emission fluxes of a test fungal species (Penicillium chrysogenum) grown on two test surfaces (aluminum foil and cardboard) in a flux chamber in response to air flow. Spore growth on the aluminum foil correlated with the amount of nutrient, while for cardboard, fungal growth was observed just with the presence of water without any external nutrients. The released spores were collected using a commercially available impinger (Biosampler ® ) and quantified using fluorescence microscopy. Spore flux correlated positively with the number of spores on the surface and with air velocity above a threshold velocity. Fungal spore flux decreased on continuous exposure to air. Microscopic inspection of the surface revealed that the normally upright hyphae bearing the fungal spores collapsed after exposure to air thus suggesting that the decrease in flux was due to a decrease in the aerodynamic drag on the spores. Fungal hyphae also collapsed when there was depletion of nutrients for spore growth leading to reduction in flux. A preliminary mathematical model that estimates spore flux based on the energy transfer between the air and the fungal spores and the energy required to remove the spores is presented. A characteristic energy parameter for aerosolisation was obtained from the model fit. Using this parameter, the model predicted experimental fluxes under various conditions reasonably well.

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Section: Introductionmentioning

confidence: 99%

Passive Release of Fungal Spores from Synthetic Solid Waste Surfaces

Gopalakrishnan

Devassikutty

Mathew

et al. 2016

Aerosol Air Qual. Res.

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“…These would have the flexibility for use in combination with various observation or detection techniques: for example, counting and characterization of stained microbes using epifluorescence microscopy (Nakajima et al ; Hara and Zhang ; Murata and Zhang ), and flow cytometry (Chen and Li ; Besmer et al ; Buysschaert et al ). Combinations of fluorescent staining with other methods also have been developed (Bisson et al ; Park et al ; Choi et al ; Yamaguchi et al ; Sorensen et al ; Swanson and Huffman ). Although the use of fluorescent staining has been reported as above, the staining conditions appear to be determined by trial and error.…”

Section: Introductionmentioning

confidence: 99%

Optimal fluorescent‐dye staining time for the real‐time detection of microbes: a study of Saccharomyces cerevisiae

et al. 2020

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Aims To provide information on the time‐dependent behaviour of microbe staining by fluorescent dyes in the order of seconds, which is important in terms of the recent rapid and online techniques for microbe measurements and/or environmental microbe analysis. Methods and Results For combinations of yeast (Saccharomyces cerevisiae) and typical dyes, including DAPI (4′,6‐diamidino‐2‐phenylindole) and Auramine‐O, a suspension of yeast cells in ultrapure water was injected into a dye solution in a micro cuvette placed inside a spectrofluorometer and the fluorescence intensity of the resulting solution was measured at 1 s intervals, starting immediately after the mixing and continued until the time for the maximum intensity using various concentrations of yeast and dyes. The relaxation time τ, which corresponds to ~63·2% of the maximum fluorescence intensity, was shown to decrease to below 1 s with increasing DAPI concentration, whereas it remained constant for 2–3 s with increasing Auramine‐O concentration, for example at a yeast concentration of 100 µg ml−1. Conclusions For the conditions of yeast >10 µg ml−1, DAPI >1 µg ml−1 and Auramine‐O >0·1 µg ml−1, τ could be adjusted to below 5 s to achieve a rapid and stable staining. Significance and Impact of the Study Design and operating conditions for rapid and online measurements of microbes can be optimized.

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“…These methods include impaction, filtration, impingement and depositional sampling. A rapid assessment method was developed to determine bacterial viability by applying ultraviolet and visible spectroscopy analysis (Park et al, 2012). However, an evaluation of sensitivity, repeatability and accuracy for bioaerosol sampling is important and it is essential to determine the capabilities and limitations of the various available sampling methods.…”

Section: Introductionmentioning

confidence: 99%

In Situ Rapid Evaluation of Indoor Bioaerosols Using an ATP Bioluminescence Assay

Lin

Wang

Hsien

et al. 2013

Aerosol Air Qual. Res.

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An adenosine-5'-triphosphate (ATP) bioluminescence method was developed for detecting microbial activity in indoor air. This method was compared with the traditional method of collecting, culturing and counting CFUs. A comparison of the results showed that ATP bioluminescence, expressed as RLUs, was moderately correlated with the entire set of CFU counts (r = 0.607), and that the correlation improved to r = 0.963 (p value < 0.001) when CFU outliers were removed from the calculations. The ATP bioluminescence method was applied at four different sites; a hospital Chinese medicine diagnostic room, a library, a government office, and a railway station lobby. The results showed that microbial activity was far higher in the railway station lobby than at the other three sites, and this is believed to be due to the higher volume and density of people in this space. At all four sites, higher microbial activity was linked to indoor plants, garbage cans, shoe racks, and furnished waiting areas. PCA of the data showed that microbial activity in the Chinese medicine diagnostic room was closely related to room temperature and humidity, and hence lowering the latter can reduce the potential for microbial activity at this site. At all four sites, no correlation was found between microbial activity and airborne pollutants. The ATP bioluminescence method was applied for the rapid evaluation of room disinfection using chloride dioxide, and results showed that twenty minutes after spraying with 100 ppm ClO 2 , microbial activity was reduced to 38.7% of its original level. ATP bioluminescence is simpler, easier to operate, and more cost-effective than the conventional microbial culture method of evaluating microbial load. The results obtained in this research confirm that the proposed ATP bioluminescence technique is capable of instantaneously detecting microbial activity in an indoor environment. Moreover, this approach can be used for on-line evaluation of room disinfection efficiency.

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Development of Rapid Assessment Method to Determine Bacterial Viability Based on Ultraviolet and Visible (UV-Vis) Spectroscopy Analysis Including Application to Bioaerosols

Cited by 29 publications

References 42 publications

Passive Release of Fungal Spores from Synthetic Solid Waste Surfaces

Passive Release of Fungal Spores from Synthetic Solid Waste Surfaces

Optimal fluorescent‐dye staining time for the real‐time detection of microbes: a study of Saccharomyces cerevisiae

In Situ Rapid Evaluation of Indoor Bioaerosols Using an ATP Bioluminescence Assay

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