Refractometry of organosilica microspheres

Seet, Katrina Y. T.; Vogel, Robert; Nieminen, Timo A.; Knöner, Gregor; Rubinsztein‐Dunlop, Halina; Trau, Matt; Zvyagin, Andrei V.

doi:10.1364/ao.46.001554

Cited by 11 publications

(6 citation statements)

References 30 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2.01-m polystyrene microspheres ͑ProSciTech, product number QPP-02-10͒, 3.27-m ͑Dako SpectrAlign, product number K0111͒, and 5.94-m organosilica microspheres were used for measurements. Two methods of refractometry, as described by Seet et al 13 were employed for the calibration of microspheres. First, a refined optical scattering spectroscopy method was applied, where a commercial laboratory Lamda40 spectrophotometer ͑Perkin-Elmer͒ was used to measure the refractive index of the biological phantoms.…”

Section: Verification Of the Dfm Techniquementioning

confidence: 99%

“…20 The two methods were compared, and very good agreement was achieved. 13 Several optical scatter spectra were measured for each type of microsphere. The FWHM and refractive index distribution of 2.01-m and 3.27-m polystyrene and 2.80-m organosilica microspheres are shown in Fig.…”

Section: Verification Of the Dfm Techniquementioning

confidence: 99%

“…The use of DFM for the determination of the refractive index and diameter of polymer beads may also assist the refinement of the existing DNA sequencing methods. 12,13 Currently, this is limited by the use of colloid dye-doped beads. 14,15 Optical scatter field recorded by DFM contains information about the object's size, shape, relative location, and refractive index.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Refractometry of melanocyte cell nuclei using optical scatter images recorded by digital Fourier microscopy

Seet

Nieminen

Zvyagin³

2009

J. Biomed. Opt.

Self Cite

View full text Add to dashboard Cite

Abstract. The cell nucleus is the dominant optical scatterer in the cell. Neoplastic cells are characterized by cell nucleus polymorphism and polychromism-i.e., the nuclei exhibits an increase in the distribution of both size and refractive index. The relative size parameter, and its distribution, is proportional to the product of the nucleus size and its relative refractive index and is a useful discriminant between normal and abnormal ͑cancerous͒ cells. We demonstrate a recently introduced holographic technique, digital Fourier microscopy ͑DFM͒, to provide a sensitive measure of this relative size parameter. Fourier holograms were recorded and optical scatter of individual scatterers were extracted and modeled with Mie theory to determine the relative size parameter. The relative size parameter of individual melanocyte cell nuclei were found to be 16.5± 0.2, which gives a cell nucleus refractive index of 1.38± 0.01 and is in good agreement with previously reported data. The relative size parameters of individual malignant melanocyte cell nuclei are expected to be greater than 16.5.

show abstract

Section: Verification Of the Dfm Techniquementioning

confidence: 99%

Section: Verification Of the Dfm Techniquementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Refractometry of melanocyte cell nuclei using optical scatter images recorded by digital Fourier microscopy

Seet

Nieminen

Zvyagin³

2009

J. Biomed. Opt.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Silica particles can also be easily functionalized by the incorporation of specific organic groups . Compared with purely inorganic silica particles typically prepared by the Stöber method using TEOS as precursor, organosilica nanoparticles have potential applications in optics, − functional materials, , and biosciences − because of their high surface reactivity, smaller negative surface charge, and enhanced biological compatibility. Organosilica particles can be readily obtained by post-synthetic grafting of organoalkoxysilanes onto preformed silica particles.…”

Section: Introductionmentioning

confidence: 99%

l-Arginine-Catalyzed Synthesis of Nanometric Organosilica Particles through a Waterborne Sol–Gel Process and Their Porous Structure Analysis

et al. 2018

View full text Add to dashboard Cite

We report an efficient and easy-to-implement waterborne sol-gel process for the synthesis of nanometric organosilica particles. In this process, tetraethyl orthosilicate (TEOS) and 3-(methacryloxy)propyl trimethoxy silane (γ-MPS), employed as silica sources, were heterogeneously delivered in an aqueous solution of l-arginine, a basic amino acid used as a catalyst, from a top organic layer. Co-condensation of TEOS with γ-MPS led to the formation of organosilica particles with diameters between 30 and 230 nm when increasing the γ-MPS content from 0 to 10.1 mol % in the silica source. Nitrogen sorption analyses confirmed the microporous nature of the obtained particles after calcination. The Brunauer-Emmett-Teller (BET) surface areas increased from 27 (before calcination) to 684 m g (after calcination) for the organosilica particles containing 10.1 mol % of γ-MPS. Fourier transform infrared spectroscopy and Si NMR were employed to analyze the chemical structure of the organosilica spheres and provide insight into the mechanism of particle formation. In the second part, hybrid organosilica particles with a core-shell morphology were synthesized through the combination of Pickering emulsion and the sol-gel process. γ-MPS emulsion droplets stabilized by tiny silica particles (formed in a separate step) were first generated and used as seeds to grow a silica shell on their surface through TEOS addition from the top organic layer. Transmission electron microscopy and pore size analyses of the resulting particles after calcination revealed a unique dual-porosity structure with a mesoporous inner core and a micro/mesoporous silica shell with ink-bottle-type pores.

show abstract

“…Organo-silica nanoparticles have recently attracted considerable interest due to their potential applications in optics [1][2][3][4][5], functional materials [6][7][8], and biosciences [9][10][11][12]. Compared with inorganic silica nanoparticles, typically prepared by the Stöber method [13], these new organo-silica nanoparticles differ in several aspects from silica particles made from tetraethyloxysilane (TEOS) alone [14].…”

Section: Introductionmentioning

confidence: 99%

A facile synthesis of highly water-soluble, core–shell organo-silica nanoparticles with controllable size via sol–gel process

Hamilton

Reilly

et al. 2009

Journal of Colloid and Interface Science

View full text Add to dashboard Cite

Refractometry of organosilica microspheres

Cited by 11 publications

References 30 publications

Refractometry of melanocyte cell nuclei using optical scatter images recorded by digital Fourier microscopy

Refractometry of melanocyte cell nuclei using optical scatter images recorded by digital Fourier microscopy

l-Arginine-Catalyzed Synthesis of Nanometric Organosilica Particles through a Waterborne Sol–Gel Process and Their Porous Structure Analysis

A facile synthesis of highly water-soluble, core–shell organo-silica nanoparticles with controllable size via sol–gel process

Contact Info

Product

Resources

About