Refractometry of melanocyte cell nuclei using optical scatter images recorded by digital Fourier microscopy

Seet, Katrina Y. T.; Nieminen, Timo A.; Zvyagin, Andrei V.

doi:10.1117/1.3207141

Cited by 9 publications

(8 citation statements)

References 24 publications

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“…Another approach is to interfere a bright field image with an external reference wave, typically in a Mach-Zehnder interferometer. In these so-called 'digital holographic microscopes' (DHMs), one can calculate the phase of a sample by using only a single image if the reference wave has a sufficiently large inclination with respect to the image wave [106][107][108][109][110][111][112][113].…”

Section: Quantitative Image Reconstructionmentioning

confidence: 99%

What spatial light modulators can do for optical microscopy

Maurer¹,

Jesacher²,

Bernet³

et al. 2010

Laser & Photonics Reviews

429

219

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With the availability of high-resolution miniature spatial light modulators (SLMs) new methods in optical microscopy have become feasible. The SLMs discussed in this review consist of miniature liquid crystal displays with micronsized pixels that can modulate the phase and/or amplitude of an optical wavefront. In microscopy they can be used to control and shape the sample illumination, or they can act as spatial Fourier filters in the imaging path. Some of these applications are reviewed in this article. One of them, called spiral phase contrast, generates isotropic edge enhancement of thin phase samples or spiral-shaped interference fringes for thicker phase samples, which can be used to reconstruct the phase topography from a single on-axis interferogram. If SLMs are used for both illumination control and spatial Fourier filtering, this combination for instance allows for the generalization of the Zernike phase contrast principle. The new SLM-based approach improves the effective resolution and avoids some shortcomings and artifacts of the traditional method. The main advantage of SLMs in microscopy is their flexibility, as one can realize various operation modes in the same setup, without the need for changing any hardware components, simply by electronically switching the phase pattern displayed on the SLMs.

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Section: Quantitative Image Reconstructionmentioning

confidence: 99%

What spatial light modulators can do for optical microscopy

Maurer¹,

Jesacher²,

Bernet³

et al. 2010

Laser & Photonics Reviews

429

219

View full text Add to dashboard Cite

show abstract

“…All A-scans in this final image are then averaged to obtain an A-scan that fully describes the group. contribution to light scattering in this layer comes from the nucleus [5], as it is the biggest organelle in the soma and presents a high refractive index difference to the surrounding medium. As such, we modeled the outer nuclear layer as a population of spherical nuclei in an homogenous medium.…”

Section: B Model and Monte Carlo Simulationmentioning

confidence: 99%

Monte Carlo simulation of diabetic macular edema changes on optical coherence tomography data

Correia

Pinto

Araújo

et al. 2014

IEEE-EMBS International Conference on Biomedical and Health Informatics (BHI)

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Optical coherence tomography (OCT) scans were acquired from healthy controls and patients with diabetic macular edema (DME), a common complication of diabetes characterized by increased retinal thickness due to fluid accumulation. The collected OCT data was divided into three distinct groups: healthy subjects, DME patients with significantly increased outer nuclear layer (ONL) thickness and DME patients without visible changes in the ONL. For each group, the ONL was segmented and processed, yielding a representative A-scan. Using reference values for the physical and optical characteristics of the healthy human retina, we used a Monte Carlo method with a model for the ONL to simulate an A-scan for each group and compare it to the real OCT data. This allowed to identify which alterations in the cellular characteristics are responsible for the changes observed in the OCT scans of the diseased groups.

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“…As methods for the evaluation of the refractive index of mammalian cells have advanced, so too has the controversy regarding whether the mean refractive indices of the nucleus and the cytoplasm differ [8][9][10][11] . Some of the first measurements were done using phase contrast microscopy by identifying the refractive index of the extracellular medium which provided no contrast with the nucleus or cytoplasm, i.e.…”

Section: Introductionmentioning

confidence: 99%

Optical propulsion of mammalian eukaryotic cells on an integrated channel waveguide

Shahimin

Perney

Brooks

et al. 2011

Microfluidics, BioMEMS, and Medical Microsystems IX

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The optical propulsion of mammalian eukaryotic cells along the surface of an integrated channel waveguide is demonstrated. 10μm diameter polymethylmethacrylate (PMMA) spherical particles and similarly sized mammalian eukaryotic cells in aqueous medium are deposited in a reservoir over a caesium ion-exchanged channel waveguide. Light from a fibre laser at 1064nm was coupled into the waveguide, causing the polymer particles or cells to be propelled along the waveguide at a velocity which is dependent upon the laser power. A theoretical model was used to predict the propulsion velocity as a function of the refractive index of the particle. The experimental results obtained for the PMMA particles and the mammalian cells show that for input powers greater than 50mW the propulsion velocity is approximately that obtained by the theoretical model. For input powers of less than ~50mW neither particles nor cells were propelled; this is considered to be a result of surface forces (which are not considered in the theoretical model).The results are discussed in light of the potential application of optical channel waveguides for bioanalytical applications, namely in the identification and sorting of mammalian cells from mixed populations without the need for fluorescence or antibody labels.

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Refractometry of melanocyte cell nuclei using optical scatter images recorded by digital Fourier microscopy

Cited by 9 publications

References 24 publications

What spatial light modulators can do for optical microscopy

What spatial light modulators can do for optical microscopy

Monte Carlo simulation of diabetic macular edema changes on optical coherence tomography data

Optical propulsion of mammalian eukaryotic cells on an integrated channel waveguide

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