Quantitative proteomic analysis of human corneal epithelial cells infected with HSV-1

Cui, Yu-Hong; Li, Qun; Xu, Zhifeng; Li, Jiahui; Hu, Zixuan; Li, Meijun; Zheng, Wei; Li, Zhijie

doi:10.1016/j.exer.2019.05.004

Cited by 16 publications

(12 citation statements)

References 36 publications

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“…The top categories for biological processes were metabolic process, antigen presentation, inflammatory response, and TNF-mediated and T cell receptor pathways. This was in line with previous virus- and cell-based studies showing that HSV-1 infection induces the innate immune response, and upregulates inflammatory factors for inhibiting viral infection [ 23 ]. An unexpectedly large number of proteins were involved in cell-cell adhesion (such as CD81), and translation (such as EIF4E).…”

Section: Resultssupporting

confidence: 90%

Comparative analysis of the tear protein profile in herpes simplex virus type 1 epithelial keratitis

Yang

Wang

et al. 2020

BMC Ophthalmol

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Background Herpes simplex virus type 1 (HSV-1) keratitis is a major cause of corneal blindness in the world, and an in-depth understanding of its pathogenesis may help improve existing diagnosis and treatment. The purpose of this study is to compare and analysis the total tear protein profile of HSV-1 epithelial keratitis patients, and to quantify the potential candidate biomarkers of HSV-1 epithelial keratitis. Methods We investigated the proteome in tear fluid from three HSV-1 epithelial keratitis patients and three healthy control subjects using nano-scale liquid chromatography-tandem mass spectrometry (nLC-MS/MS) analysis. Functional annotation of differentially expressed proteins was done with the Gene Ontology (GO) analysis. ELISA was done to quantify the potential candidate biomarkers in 26 clinical cases. Results Tear fluid from three HSV-1 epithelial keratitis patients and three healthy control subjects contained a total of 1275 proteins and 326 proteins were unique to tear fluid of HSV-1 epithelial keratitis patients. Bioinformatics analysis revealed that tear proteins from HSV-1 epithelial keratitis patients may be involved in metabolic processes, antigen presentation, inflammatory response, and in the TNF-mediated and T cell receptor pathways. Furthermore, IL1A, IL12B, DEFB4A, and CAMP, which are associated with the inflammatory response and inhibition of viral infection, were significantly more abundant in the HSV-1 epithelial keratitis patients than in the healthy control subjects. Conclusions This study reports the proteomic profile of tears in HSV-1 epithelial keratitis for the first time and identifies a number of unique differentially expressed proteins.

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Section: Resultssupporting

confidence: 90%

Comparative analysis of the tear protein profile in herpes simplex virus type 1 epithelial keratitis

Yang

Wang

et al. 2020

BMC Ophthalmol

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“…Metabolomic study, on the other hand, revealed that HSV-1 does not significantly affect glycolysis but induces pyruvate carboxylation to anaplerotically replenish TCA cycle metabolites, while redirecting other TCA cycle intermediates to pyrimidine biosynthesis (Vastag et al, 2011). Recent proteomic analysis of human corneal epithelial cells infected with HSV-1 showed a significant metabolic shift characterized by decreased TCA cycle activity and increased glycolysis upon infection (Cui et al, 2019). Since modulation of glycolysis during HSV-1 infection is variable under different circumstances, further studies are needed to decipher the mechanisms involved in virus-induced metabolic reprogramming and to determine whether glucose metabolism is important for HSV-1 replication.…”

Section: Dna Virusesmentioning

confidence: 99%

Metabolic reprogramming as a feast for virus replication

Polcicova¹,

Baďurová²,

Tomaskova³

2020

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“…By comparing temporal proteome changes during WT and d106 HSV-1 infections, we discovered the upregulation of several proteins involved in innate immunity and apoptosis, and integration with cGAS IP-MS led to the discovery of OASL-mediated cGAS inhibition [51]. Additional MS studies have been carried out to characterize proteome changes during HSV-1 infection in a range of cell types and to compare alterations induced by different virus strains [51,54,104,[111][112][113][114][115][116][117][118][119][120][121]. Spatial proteomics [122] has further provided the ability to characterize changes in proteome organization during infection [123], as well as discover viral proteins that localize to distinct organelles to regulate their functions, as shown for HCMV infection [124].…”

Section: Defining the Cellular Landscape Representative Of Immune Activationmentioning

confidence: 99%

Interrogating Host Antiviral Environments Driven by Nuclear DNA Sensing: A Multiomic Perspective

Howard

Cristea

2020

Biomolecules

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Nuclear DNA sensors are critical components of the mammalian innate immune system, recognizing the presence of pathogens and initiating immune signaling. These proteins act in the nuclei of infected cells by binding to foreign DNA, such as the viral genomes of nuclear-replicating DNA viruses herpes simplex virus type 1 (HSV-1) and human cytomegalovirus (HCMV). Upon binding to pathogenic DNA, the nuclear DNA sensors were shown to initiate antiviral cytokines, as well as to suppress viral gene expression. These host defense responses involve complex signaling processes that, through protein–protein interactions (PPIs) and post-translational modifications (PTMs), drive extensive remodeling of the cellular transcriptome, proteome, and secretome to generate an antiviral environment. As such, a holistic understanding of these changes is required to understand the mechanisms through which nuclear DNA sensors act. The advent of omics techniques has revolutionized the speed and scale at which biological research is conducted and has been used to make great strides in uncovering the molecular underpinnings of DNA sensing. Here, we review the contribution of proteomics approaches to characterizing nuclear DNA sensors via the discovery of functional PPIs and PTMs, as well as proteome and secretome changes that define a host antiviral environment. We also highlight the value of and future need for integrative multiomic efforts to gain a systems-level understanding of DNA sensors and their influence on epigenetic and transcriptomic alterations during infection.

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Quantitative proteomic analysis of human corneal epithelial cells infected with HSV-1

Cited by 16 publications

References 36 publications

Comparative analysis of the tear protein profile in herpes simplex virus type 1 epithelial keratitis

Comparative analysis of the tear protein profile in herpes simplex virus type 1 epithelial keratitis

Metabolic reprogramming as a feast for virus replication

Interrogating Host Antiviral Environments Driven by Nuclear DNA Sensing: A Multiomic Perspective

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