Quantitative pharmacokinetic evaluation of Subtilisin QK-2 after a bolus IV injection in a rat model using a novel sandwich enzyme-linked immunosorbent assay

Song, Xinshan; Yang, Yuanyuan; Liang, Ning; Yang, Fan; Song, Cuihua; Zhou, Li; Zhou, Kangping; Wang, Yefu

doi:10.1016/j.jpba.2020.113264

Cited by 4 publications

(3 citation statements)

References 19 publications

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“…The enzymatic activity of nattokinase was determined using UV spectrophotometry, according to the Nattokinase Association of Japan [ 19 ]. Fibrin plates were used to verify enzyme production.…”

Section: Methodsmentioning

confidence: 99%

High-Efficiency Fermentation of Nattokinase by Recombinant PSP2 Using Oyster Protein Hydrolysate as a Substrate

Tian

Chen

Peng

et al. 2023

Foods

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In recent years, cardiovascular and cerebrovascular diseases have been the focus of several studies. In this study, oyster protein hydrolysate was produced via enzyme hydrolysis and used as a fermentation substrate to ferment recombinant strain PSP2 to produce nattokinase. Using the synergism strategy, fermentation products with fibrinolytic and angiotensin I-converting enzyme (ACE) inhibitory activities were obtained and evaluated. The fermentation medium contained 1.0% trypsin, 1.0% oyster protein hydrolysate, 2.0% maltose, and 0.5% sodium chloride, with an initial pH of 7.0. The maximum nattokinase activity was 390.23 ± 10.24 FU/mL after 72 h of fermentation. The flavor of the product was improved, and heavy metals and volatile salt nitrogen were partially removed via fermentation. The ACE inhibitory activity (IC50) of the fermentation products was 1.433 mg/mL. This study provides a novel approach for the development of marine functional foods with hypotensive and antithrombotic properties.

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Section: Methodsmentioning

confidence: 99%

High-Efficiency Fermentation of Nattokinase by Recombinant PSP2 Using Oyster Protein Hydrolysate as a Substrate

Tian

Chen

Peng

et al. 2023

Foods

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“…The three amino acid residues (Ser221, His64 and Asp32) necessary for the catalytic center of serine proteases and the region near the catalytic triad are highly conserved among the above alkaline serine protease family members ( Figure 1 ) [ 38 ]. Although nattokinase is highly homologous to many subtilisins in the serine protease family, only a few proteins, such as nattokinase, show high substrate specificity to fibrin and can directly cleave cross-linked fibrin in vitro and in vivo [ 39 ].…”

Section: Structure and Catalytic Mechanism Of Nattokinasementioning

confidence: 99%

Biotechnology, Bioengineering and Applications of Bacillus Nattokinase

Chen

2022

Biomolecules

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Thrombosis has threatened human health in past decades. Bacillus nattokinase is a potential low-cost thrombolytic drug without side-effects and has been introduced into the consumer market as a functional food or dietary supplement. This review firstly summarizes the biodiversity of sources and the fermentation process of nattokinase, and systematically elucidates the structure, catalytic mechanism and enzymatic properties of nattokinase. In view of the problems of low fermentation yield, insufficient activity and stability of nattokinase, this review discusses the heterologous expression of nattokinase in different microbial hosts and summarizes the protein and genetic engineering progress of nattokinase-producing strains. Finally, this review summarizes the clinical applications of nattokinase.

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“…The three amino acid residues (Ser221, His64 and Asp32) necessary for the catalytic center of serine proteases and the region near the catalytic triad are highly conserved among the above alkaline serine protease family members (Figure 1) [48]. Although nattokinase is highly homologous to many subtilisins in the serine protease family, Only a few proteins, such as nattokinase, show high substrate specificity to fibrin, which can directly cleave cross-linked fibrin in vitro and in vivo [49]. polypeptide without disulfide bonds.…”

Section: Nattokinase: the Only Member Of The Alkaline Serine Protease...mentioning

confidence: 99%

Biotechnology, Bioengineering and Applications of <em>Bacillus </em>Nattokinase

Li¹,

Chen²,

Li³

2022

Preprint

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Thrombosis has threatened human health in past decades. Bacillus nattokinase is a potential thrombolytic drug without side-effect and low cost and has been introduced into the consumer market as a functional food or dietary supplement. This review firstly summarizes the biodiversity sources and fermentation process of nattokinase, and systematically expounds the structure, cata-lytic mechanism and enzymatic properties of nattokinase; In view of the problems of low fermen-tation yield, insufficient activity and stability of nattokinase, this review discusses the heterologous expression of nattokinase in different microbial hosts, and summarizes the protein and genetic en-gineering progress of nattokinase-producing strains; Finally, this review summarizes the clinical application of nattokinase.

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Quantitative pharmacokinetic evaluation of Subtilisin QK-2 after a bolus IV injection in a rat model using a novel sandwich enzyme-linked immunosorbent assay

Cited by 4 publications

References 19 publications

High-Efficiency Fermentation of Nattokinase by Recombinant PSP2 Using Oyster Protein Hydrolysate as a Substrate

High-Efficiency Fermentation of Nattokinase by Recombinant PSP2 Using Oyster Protein Hydrolysate as a Substrate

Biotechnology, Bioengineering and Applications of Bacillus Nattokinase

Biotechnology, Bioengineering and Applications of <em>Bacillus </em>Nattokinase

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