High-Efficiency Fermentation of Nattokinase by Recombinant PSP2 Using Oyster Protein Hydrolysate as a Substrate

Abstract

In recent years, cardiovascular and cerebrovascular diseases have been the focus of several studies. In this study, oyster protein hydrolysate was produced via enzyme hydrolysis and used as a fermentation substrate to ferment recombinant strain PSP2 to produce nattokinase. Using the synergism strategy, fermentation products with fibrinolytic and angiotensin I-converting enzyme (ACE) inhibitory activities were obtained and evaluated. The fermentation medium contained 1.0% trypsin, 1.0% oyster protein hydrolysate, 2.0% maltose, and 0.5% sodium chloride, with an initial pH of 7.0. The maximum nattokinase activity was 390.23 ± 10.24 FU/mL after 72 h of fermentation. The flavor of the product was improved, and heavy metals and volatile salt nitrogen were partially removed via fermentation. The ACE inhibitory activity (IC50) of the fermentation products was 1.433 mg/mL. This study provides a novel approach for the development of marine functional foods with hypotensive and antithrombotic properties.

Nutrition and Cardiovascular Disease: The Potential Role of Marine Bioactive Proteins and Peptides in Thrombosis Prevention

“…This increases the synthesis of thrombolytic factors, amplifies thrombolytic activity, and enhances fibrin dissolution efficiency. Tian et al utilized oyster protein hydrolysate as a fermentation substrate to produce nattokinase, which has been studied for its potential fibrinolytic (blood-clot-dissolving) activity …”

“…Tian et al utilized oyster protein hydrolysate as a fermentation substrate to produce nattokinase, which has been studied for its potential fibrinolytic (blood-clot-dissolving) activity. 49 2.2. Protein-Based Antithrombotic Agents from Seaweed and Microalgae.…”

High Production of Nattokinase via Fed-Batch Fermentation of the γ-PGA-Deficient Strain of Bacillus licheniformis

“…Previously, NK-producing Bacillus strains were mainly isolated from fermented food or the natural environment [13][14][15][16], yet the fibrinolytic enzyme activities generated by these strains fell short of matching the requirements of industrial production. With the advent of genetic and protein engineering, Bacillus has been employed in recent years as a host strain to express the endogenous or exogenous aprN gene, which codes for NK [17,18]. The components involved in expression and transport have been further optimized [19][20][21][22].…”