Quantitative Gas Chromatographic-Mass Spectrometric Assay of Five Sulfonamide Residues in Animal Tissue

Abstract

A gas chromatographic-mass spectrometric procedure using isotopically labeled internal standards and the technique of selective-ion monitoring was used to assay sulfamethazine, sulfadimethoxine, sulfabromethazine, sulfathiazole, and sulfaquinoxaline in liver and muscle tissue of swine, poultry, and cattle. The data presented clearly demonstrate the applicability of this procedure for confirmation of sulfonamide residues in animal tissue.

Nanoscale separations combined with electrospray ionization mass spectrometry: Sulfonamide determination

“…Many methods of high performance liquid chromatography (HPLC) have been investigated employing a variety of stationary phases and mobile phases for the determination of mixtures of sulfonamide standards, sulfonamides and their metabolites, and in a variety of matrices [4,8,11,[16][17][18]. Ultraviolet and fluorescence detectors have been most routinely used but, where interferences have arisen, conhrmation of analyte identity has been obtained through gas chromatography (GC)/MS, thus introducing problems associated with GC analysis [19). Use of LC direct liquid introduction/MS/MS with an atmospheric pressure ionization source has been reported for the determination of these compounds.…”

Sulfachlorpyrazine residues depletion in turkey edible tissues

“…Various instrumental techniques have been introduced for the determination of sulfonamide residues, such as thin-layer chromatography (Thomas et al, 1983), the immuno-enzymatic method (Nouws et al, 1985), supercritical fluid chromatography (Guggisberg et al, 1992), and gas chromatography coupled with mass spectrometry (Simpson et al, 1985). However, the most prominent method is high-pressure liquid chromatography (HPLC).…”

“…, 1985), supercritical fluid chromatography (Guggisberg et al. , 1992), and gas chromatography coupled with mass spectrometry (Simpson et al. , 1985).…”

HPLC method for the simultaneous analysis of sulfadimethoxine and ormetoprim in tissues and blood of cattle, chickens, and catfish

“…(2) paper or thin-layer chromatography using either a Bratton-Marshall (Parks, 1985) or fluorescamine (Thomas et al, 1983a,b) spray for quantitation; (3) gas-liquid chromatography following derivatization with diazomethane and/or pentafluoropropionic anhydride (PFPA) with detection either by electron capture (Goodspeed et al, 1978) or selected ion mass spectrometry either in the positive chemical ionization mode using deuterium-labeled internal standard (Garland et al, 1980) or in the electron impact mode using 13C-labeled internal standard (Simpson et al, 1985).…”