HPLC method for the simultaneous analysis of sulfadimethoxine and ormetoprim in tissues and blood of cattle, chickens, and catfish

Weiss, George B.; Duke, Paul D.; Gonzales, Leonard

doi:10.1021/jf00078a013

Cited by 13 publications

(5 citation statements)

References 10 publications

(13 reference statements)

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“…This problem could theoretically be overcome by substituting ethyl acetate with dichloromethane and adding a positive counter ion to the negatively charged sulfamethoxazole. The efficacy of this approach has been demonstrated for the isolation of ormetoprim and sulfadimethoxine from tissues (20). Alternatively, solid-phase extraction on Sep Pak C18 from fish has recently been reported for this combination (19).…”

Section: Chemicalsmentioning

confidence: 99%

“…The use of two close structural analogs of the analytes as internal standards undoubtedly contributed to the excellent reproducibility of the method. Other methods either do not use an internal standard (20) or use a compound that is not structurally related to either of the therapeutic agents of interest (19). Intra-assay coefficients of variation were 2.2% (X = 79.6 ,ug/g; n = 8) for trimethoprim and 1.9% (X = 257 ,ug/g; n = 8) for sulfamethoxazole.…”

Section: Chemicalsmentioning

confidence: 99%

“…Prophylactic treatment of fish currently relies on the addition of chemotherapeutic agents to the feed (1,19,20). However, this approach is poorly effective and uneconomical, and it harbors risks for the environment and public health (2).…”

mentioning

confidence: 99%

“…Nor do high-pressure liquid chromatographic (HPLC) methods exist for the simultaneous determination of * Corresponding author. trimethoprim and sulfamethoxazole in fish, as opposed to that of the analogous drugs ormetoprim and sulfadimethoxine (19,20). The latter two compounds have recently been determined in A. franciscana by a crude microbiological assay (10).…”

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confidence: 99%

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Liquid chromatographic determination of efficacy of incorporation of trimethoprim and sulfamethoxazole in brine shrimp (Artemia spp.) used for prophylactic chemotherapy of fish

Nelis

Leger

Sorgeloos

et al. 1991

Antimicrob Agents Chemother

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,The brine shrimp Artemia, an excellent live food source in aquaculture, has been studied as a carrier to deliver selected chemotherapeutic agents to fish for prophylactic treatment of infectious disases. To monitor the efficiency of incorporation of trimethoprim and sulfamethoxazole in Artemia franciscana, a sensitive and specific analytical method was developed. It is based on homogenization of Artemia nauplii in methanol, extraction of lipids with hexane, solid-phase cleanup on C18 cartridges, and reversed-phase liquid chromatography wvith detection at 210 nm. The method is sensitive (detection limit, on the order of 3 pg/g with a sample quantity of 30 mg [dry weight]) and reproducible (coefficients of variation, 2.2 and 1.8% for trimethoprim and sulfamethoxazole at levels of 79.6 and 257 ,ug/g of body weight, respectively). Preliminary quattative data indicated excellent uptake and persistence of both therapeutic agents in A. franciscana, with levels of 115 ,ug/g for trimethoprim and 277 ,ug/g for sulfamethoxazole.

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Section: Chemicalsmentioning

confidence: 99%

Section: Chemicalsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Liquid chromatographic determination of efficacy of incorporation of trimethoprim and sulfamethoxazole in brine shrimp (Artemia spp.) used for prophylactic chemotherapy of fish

Nelis

Leger

Sorgeloos

et al. 1991

Antimicrob Agents Chemother

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“…Ormetoprim analysis followed the HPLC procedure of Weiss et al (1987). A 0.5-mL aliquot of urine was mixed with pH 10 buffer (Fisher Scientific, Fair Lawn, NJ) and extracted with 1 mL of methylene chloride by vortex mixing for 2 min.…”

Section: Analytical Proceduresmentioning

confidence: 99%

Tissue Distribution and Renal Excretion of Ormetoprim after Intravascular and Oral Administration in the Channel Catfish (Ictalurus punctatus)

Plakas¹,

Dickey²,

Barron³

et al. 1990

Can. J. Fish. Aquat. Sci.

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1990. Tissue distribution and renal excretion of ormetoprim after intravascular and oral administration in the channel catfish (Ictalurus punctatus). Can. 1. Fish. Aquat. Sci. 47: 766-771.Ormetoprim is used to potentiate sulfadimethoxine in treating certain bacterial diseases of aquatic species. The tissue disposition and renal excretion of ormetoprim and metabolites were examined after intravascular and oral administration (4 mg-kg-') in channel catfish (Ictalurus punctatus). Peak plasma level (0.66 p,g.mL-') of 14C-ormetoprim occurred at 6 h after oral dosing. The oral bioavailability was estimated at 52%. Ormetoprim and metabolites were widely distributed in the tissues. The tissue concentrations were highest in the liver, trunk kidney, head kidney, and spleen. Clearance of the radiolabel from tissues was rapid. The muscle contained 49.3% of the intravascularly administered dose at 2 h; however, at 72 h, less than 1 % of the dose remained in this tissue. 14C-Ormetoprim was more persistent in the skin than in the muscle. Ormetoprim was extensively metabolized in catfish. After intravascular administration, 21 . l % of the dose of 14C-ormetoprim was eliminated in the urine in 48 h, predominantly as polar metabolites; less than 4% of the dose was eliminated as the parent compound. Biliary excretion was a minor route of elimination (5-6% of the dose). The data suggest branchial excretion of ormetoprim and/or metabolites.L'ormetoprim est un activateur de la sulfadimethoxine dans le traitement de certaines infections bactkriennes d'especes aquatiques. Le dep6t dans les tissus et I'excretion renale de I'ormetoprim et de ses metabolites ont 6t6 suivis apr6s I'administration par voie orale ou intravasculaire (4 mg-kg-') A des barbues de rivi6re (Ictalurus punctatus). La concentration maximale dans le plasma (0,66 pg.mL-l) de 14C-ormetoprim a et6 observee 6h apres administration par voie orale. La biodisponibilite du produit administre sous forme orale a 6te kvaluee A 52 %. L'ormetoprim et ses metabolites sont largement distribues dans les tissus. Les concentrations tissulaires le plus devees ont ete observees dans le foie, I'opistonephros, le pronephros et la rate. La clearance tissulaire du produ it radiomarque est rapide. Deux heures apr6s I'admin istration intravasculaire, le tissu musculaire contenait 49,3 % de la dose; apr6s 72 h cependant, ce tissu n'en contenait plus que moins d'un p. cent. Le 14C-ormetoprim s'est revel6 plus persistant dans la peau que dans le tissu musculaire. L'ormetoprim est largement metabolise chez la barbue. Apres I'administration intravasculaire, 21,l % du 14C-ormetoprim est dimin6 dans I'urine au bout de 48 h, principalement sous forme de metabolites polaires. Moins de 4 % de la dose est elimine sous la forme du compose-parent. L'excretion biliaire constitue une voie mineure d'elin-ina at ion (5 A 6 % de la dose administree).Les resultats indiquent qu'il se produit une excretion branchiale de 110rm6toprim ou de ses metabolites.

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Determination of sulfonamides by liquid chromatography, ultraviolet diode array detection and ion-spray tandem mass spectrometry with application to cultured salmon flesh

Pleasance

Blay

Quilliam

et al. 1991

Journal of Chromatography A

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HPLC method for the simultaneous analysis of sulfadimethoxine and ormetoprim in tissues and blood of cattle, chickens, and catfish

Cited by 13 publications

References 10 publications

Liquid chromatographic determination of efficacy of incorporation of trimethoprim and sulfamethoxazole in brine shrimp (Artemia spp.) used for prophylactic chemotherapy of fish

Liquid chromatographic determination of efficacy of incorporation of trimethoprim and sulfamethoxazole in brine shrimp (Artemia spp.) used for prophylactic chemotherapy of fish

Tissue Distribution and Renal Excretion of Ormetoprim after Intravascular and Oral Administration in the Channel Catfish (Ictalurus punctatus)

Determination of sulfonamides by liquid chromatography, ultraviolet diode array detection and ion-spray tandem mass spectrometry with application to cultured salmon flesh

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