Quantitative Evaluation of Human Umbilical Vein and Induced Pluripotent Stem Cell-Derived Endothelial Cells as an Alternative Cell Source to Skin-Specific Endothelial Cells in Engineered Skin Grafts

Pappalardo, Alberto; Herron, Lauren; Cespedes, David Alvarez; Abaci, Hasan Erbil

doi:10.1089/wound.2020.1163

Cited by 11 publications

(13 citation statements)

References 42 publications

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“…Thanks to their enclosed and customizable shapes, these anatomically modeled 3D skin substitutes can be transplanted as a single wearable piece, minimizing the need for suturing, substantially reducing the length of surgeries, and improving the esthetic outcome by eliminating the scarring between multiple graft patches. In addition, the method allows to easily incorporate a vascular network, an important feature for the integration of the grafts onto the host, as we and others previously demonstrated (2,25,32,33). This technology does not require more cells than a conventional skin graft and therefore is clinically feasible to cover large areas.…”

Section: Discussionmentioning

confidence: 94%

“…We tested the wearable edgeless dermal constructs (WDCs) side by side with conventional dermal constructs (CDCs) that we routinely produce and use in our studies (see Materials and Methods for the details for CDCs) ( 1 – 3 , 25 , 26 ). After 14 days of remodeling, the CDC was contracted, with 10 to 50% reduction in the original radius and roughly 80% in the thickness ( Fig.…”

Section: Resultsmentioning

confidence: 99%

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Engineering edgeless human skin with enhanced biomechanical properties

et al. 2023

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Despite the advancements in skin bioengineering, 3D skin constructs are still produced as flat tissues with open edges, disregarding the fully enclosed geometry of human skin. Therefore, they do not effectively cover anatomically complex body sites, e.g., hands. Here, we challenge the prevailing paradigm by engineering the skin as a fully enclosed 3D tissue that can be shaped after a body part and seamlessly transplanted as a biological clothing. Our wearable edgeless skin constructs (WESCs) show enhanced dermal extracellular matrix (ECM) deposition and mechanical properties compared to conventional constructs. WESCs display region-specific cell/ECM alignment, as well as physiologic anisotropic mechanical properties. WESCs replace the skin in full-thickness wounds of challenging body sites (e.g., mouse hindlimbs) with minimal suturing and shorter surgery time. This study provides a compelling technology that may substantially improve wound care and suggests that the recapitulation of the tissue macroanatomy can lead to enhanced biological function.

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Section: Discussionmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Engineering edgeless human skin with enhanced biomechanical properties

et al. 2023

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“…2). The biomaterials used for scaffolds can be classified into natural (natural ECM, polysaccharides, and proteins) [39][40][41] and synthetic ( polycaprolactone, polyvinyl alcohol, polyethylene glycol, etc.) [42][43][44] biomaterials.…”

Section: Scaffold-based Methodsmentioning

confidence: 99%

Strategies for vascularized skin modelsin vitro

et al. 2022

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“…Nevertheless, and specifically pointing at the hESCderived endothelial cells, these mostly resemble the ones in embryonic developmental stages [134]. In this regard, Pappalardo et al specifically observed how the maturity of human dermal blood endothelial cells stood out in the maintenance of vascularized skin grafts in the long term, compared with hPSC-derived endothelial cells and HUVECs [141]. The best way to determine the degree of validation for these endothelial cells would be to have the full genetic and phenotypic description of what each organ-specific endothelial cell should be like, but this knowledge is still limited.…”

Section: Organ-specific Endothelial Cell Culture and Phenotypic Driftmentioning

confidence: 99%

Organ-Specific Endothelial Cell Differentiation and Impact of Microenvironmental Cues on Endothelial Heterogeneity

Gifre-Renom

Daems

Luttun

et al. 2022

IJMS

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Endothelial cells throughout the body are heterogeneous, and this is tightly linked to the specific functions of organs and tissues. Heterogeneity is already determined from development onwards and ranges from arterial/venous specification to microvascular fate determination in organ-specific differentiation. Acknowledging the different phenotypes of endothelial cells and the implications of this diversity is key for the development of more specialized tissue engineering and vascular repair approaches. However, although novel technologies in transcriptomics and proteomics are facilitating the unraveling of vascular bed-specific endothelial cell signatures, still much research is based on the use of insufficiently specialized endothelial cells. Endothelial cells are not only heterogeneous, but their specialized phenotypes are also dynamic and adapt to changes in their microenvironment. During the last decades, strong collaborations between molecular biology, mechanobiology, and computational disciplines have led to a better understanding of how endothelial cells are modulated by their mechanical and biochemical contexts. Yet, because of the use of insufficiently specialized endothelial cells, there is still a huge lack of knowledge in how tissue-specific biomechanical factors determine organ-specific phenotypes. With this review, we want to put the focus on how organ-specific endothelial cell signatures are determined from development onwards and conditioned by their microenvironments during adulthood. We discuss the latest research performed on endothelial cells, pointing out the important implications of mimicking tissue-specific biomechanical cues in culture.

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Quantitative Evaluation of Human Umbilical Vein and Induced Pluripotent Stem Cell-Derived Endothelial Cells as an Alternative Cell Source to Skin-Specific Endothelial Cells in Engineered Skin Grafts

Cited by 11 publications

References 42 publications

Engineering edgeless human skin with enhanced biomechanical properties

Engineering edgeless human skin with enhanced biomechanical properties

Strategies for vascularized skin modelsin vitro

Organ-Specific Endothelial Cell Differentiation and Impact of Microenvironmental Cues on Endothelial Heterogeneity

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