Strategies for vascularized skin models<i>in vitro</i>

Gao, Chuang; Lü, Chunxiang; Qiao, Hao; Zhang, Yi; Liu, Huazhen; Jian, Zhian; Guo, Zilong; Liu, Yuanyuan

doi:10.1039/d2bm00784c

Cited by 12 publications

(6 citation statements)

References 117 publications

(118 reference statements)

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“…PCL is a biodegradable synthetic polymer with a low melting point, good solubility, biocompatibility, stability, and mechanical properties. These properties make it a popular material in tissue engineering [21][22][23]. Furthermore, PCL has been approved by the U.S. Food and Drug Administration (FDA) [24,25].…”

Section: Of 18mentioning

confidence: 99%

A Novel Method for Fabricating the Undulating Structures at Dermal—Epidermal Junction by Composite Molding Process

Qiao,

Gao,

et al. 2024

JFB

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The dermal–epidermal junction (DEJ), located between the dermal–epidermal layers in human skin tissue, plays a significant role in its function. However, the limitations of biomaterial properties and microstructure fabrication methods mean that most current tissue engineered skin models do not consider the existence of DEJ. In this study, a nanofiber membrane that simulates the fluctuating structure of skin DEJ was prepared by the composite molding process. Electrospinning is a technique for the production of nanofibers, which can customize the physical and biological properties of biomaterials. At present, electrospinning technology is widely used in the simulation of customized natural skin DEJ. In this study, four different concentration ratios of poly (lactic-co-glycolic acid) (PLGA) and polycaprolactone (PCL) nanofiber membranes were prepared based on electrospinning technology. We selected a 15%PLGA + 5%PCL nanofiber membrane with mechanical properties, dimensional stability, hydrophilicity, and biocompatibility after physical properties and biological characterization. Then, the array-based microstructure model was prepared by three-dimensional (3D) printing. Subsequently, the microstructure was created on a 15%PLGA + 5%PCL membrane by the micro-imprinting process. Finally, the cell proliferation and live/dead tests of keratinocytes (HaCaTs) and fibroblasts (HSFs) were measured on the microstructural membrane and flat membrane. The results showed that 15%PLGA + 5%PCL microstructure membrane was more beneficial to promote the adhesion and proliferation of HaCaTs and HSFs than a flat membrane.

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Section: Of 18mentioning

confidence: 99%

A Novel Method for Fabricating the Undulating Structures at Dermal—Epidermal Junction by Composite Molding Process

Qiao,

Gao,

et al. 2024

JFB

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“…In addition, the success rate of prevascular skin models used for in vitro testing will also increase. 84 Therefore, the pre-angiogenic approach is usually the preferred method.…”

Section: Sources Of Modeled Skinmentioning

confidence: 99%

Advancements in microfluidics for skin cosmetic screening

Cheng

Zhang

et al. 2023

Analyst

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“…3,4 As a key part of tissue engineering, scaffolds can be able to mimic the environment of tissue repair and cell growth and tissue repair and transmit biological, chemical, and physical informations. 5 Electrical stimulations effectively regulate cell behaviors has been revealed. It is well-known that electrical stimulations greatly regulate cellular behavior by regulating ion channels and potentials on the cell membrane, activating voltage-sensitive channels and receptors, and regulating gene expression.…”

Section: Introductionmentioning

confidence: 99%

“…Trauma, diseases, medical surgery, and sports can lead to damage and loss of biological tissues and organs. , Tissue engineering involves implanting scaffolds prepared in vitro into defect sites to guide and stimulate new tissue formation and organ repair, overcoming issues of insufficient supply and immune rejection caused by autografts and allografts. , As a key part of tissue engineering, scaffolds can be able to mimic the environment of tissue repair and cell growth and tissue repair and transmit biological, chemical, and physical informations . Electrical stimulations effectively regulate cell behaviors has been revealed.…”

Section: Introductionmentioning

confidence: 99%

Scaffold-Based Poly(Vinylidene Fluoride) and Its Copolymers: Materials, Fabrication Methods, Applications, and Perspectives

Sun,

Gao,

Liu

et al. 2024

ACS Biomater. Sci. Eng.

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Tissue engineering involves implanting grafts into damaged tissue sites to guide and stimulate the formation of new tissue, which is an important strategy in the field of tissue defect treatment. Scaffolds prepared in vitro meet this requirement and are able to provide a biochemical microenvironment for cell growth, adhesion, and tissue formation. Scaffolds made of piezoelectric materials can apply electrical stimulation to the tissue without an external power source, speeding up the tissue repair process. Among piezoelectric polymers, poly-(vinylidene fluoride) (PVDF) and its copolymers have the largest piezoelectric coefficients and are widely used in biomedical fields, including implanted sensors, drug delivery, and tissue repair. This paper provides a comprehensive overview of PVDF and its copolymers and fillers for manufacturing scaffolds as well as the roles in improving piezoelectric output, bioactivity, and mechanical properties. Then, common fabrication methods are outlined such as 3D printing, electrospinning, solvent casting, and phase separation. In addition, the applications and mechanisms of scaffold-based PVDF in tissue engineering are introduced, such as bone, nerve, muscle, skin, and blood vessel. Finally, challenges, perspectives, and strategies of scaffold-based PVDF and its copolymers in the future are discussed.

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Strategies for vascularized skin modelsin vitro

Abstract: As the largest organ of the human body, the skin has a complex multi-layered structure. The composition of the skin includes cells, extracellular matrix (ECM), vascular networks, and other appendages....

Cited by 12 publications

References 117 publications

A Novel Method for Fabricating the Undulating Structures at Dermal—Epidermal Junction by Composite Molding Process

A Novel Method for Fabricating the Undulating Structures at Dermal—Epidermal Junction by Composite Molding Process

Advancements in microfluidics for skin cosmetic screening

Scaffold-Based Poly(Vinylidene Fluoride) and Its Copolymers: Materials, Fabrication Methods, Applications, and Perspectives

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