Engineering edgeless human skin with enhanced biomechanical properties

Pappalardo, Alberto; Cespedes, David Alvarez; Fang, Shuyang; Herschman, Abigail R.; Jeon, Eun Young; Myers, Kristin M.; Kysar, Jeffrey W.; Abaci, Hasan Erbil

doi:10.1126/sciadv.ade2514

Cited by 8 publications

(7 citation statements)

References 59 publications

(85 reference statements)

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“…Recently, an edgeless collagen‐based skin tissue was fabricated without any open edges. [ 101 ] Most skin models are characterized by open boundaries, which stands in contrast with the enclosed arrangement of native skin. The process of edgeless skin formation starts by generating a computer‐aided model of a body part.…”

Section: Strategies To Fabricate Vascularized Skin Modelsmentioning

confidence: 99%

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Vascularized 3D Human Skin Models in the Forefront of Dermatological Research

Rimal,

Muduli,

Desai

et al. 2024

Adv Healthcare Materials

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In vitro engineered skin models are emerging as an alternative platform to reduce and replace animal testing in dermatological research. Despite the progress made in recent years, considerable challenges still exist for the inclusion of diverse cell types within skin models. Blood vessels, in particular, are essential in maintaining tissue homeostasis and are one of many primary contributors to skin disease inception and progression. Substantial efforts in the past have allowed the successful fabrication of vascularized skin models that are currently utilized for disease modelling and drugs/cosmetics testing. This review first discusses the need for vascularization within tissue‐engineered skin models, highlighting their role in skin grafting and disease pathophysiology. Secondly, the review spotlights the milestones and recent progress in the fabrication and utilization of vascularized skin models. Additionally, advances including the use of bioreactors, organ‐on‐a‐chip devices, and organoid systems are briefly explored. Finally, the challenges and future outlook for vascularized skin models are addressed.This article is protected by copyright. All rights reserved

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Section: Strategies To Fabricate Vascularized Skin Modelsmentioning

confidence: 99%

“…E2) Interconnections between BVs (green, CD31) and fibroblasts (red, vimentin) were observed within the vascular islands. Images are reproduced with permission from, [ 101 ] Copyright 2023, Distributed under a Creative Commons Attribution License 4.0 (CC‐BY).…”

Section: Strategies To Fabricate Vascularized Skin Modelsmentioning

confidence: 99%

Vascularized 3D Human Skin Models in the Forefront of Dermatological Research

Rimal,

Muduli,

Desai

et al. 2024

Adv Healthcare Materials

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“…This approach effectively reduces scar formation and mitigates the issue of immune rejection, providing a valuable tool in the field of regenerative medicine [ 81 , 126 ]. In 2023, Pappalardo et al [ 127 ] reported a groundbreaking study on the creation of wearable edgeless skin constructs using 3D printing, which minimizes the need for suturing and improves the effective coverage of wounds.…”

Section: Applications and Prospectsmentioning

confidence: 99%

Bioengineered skin organoids: from development to applications

Hong,

Zhu,

et al. 2023

Military Med Res

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Significant advancements have been made in recent years in the development of highly sophisticated skin organoids. Serving as three-dimensional models that mimic human skin, these organoids have evolved into complex structures and are increasingly recognized as effective alternatives to traditional culture models and human skin due to their ability to overcome the limitations of two-dimensional systems and ethical concerns. The inherent plasticity of skin organoids allows for their construction into physiological and pathological models, enabling the study of skin development and dynamic changes. This review provides an overview of the pivotal work in the progression from 3D layered epidermis to cyst-like skin organoids with appendages. Furthermore, it highlights the latest advancements in organoid construction facilitated by state-of-the-art engineering techniques, such as 3D printing and microfluidic devices. The review also summarizes and discusses the diverse applications of skin organoids in developmental biology, disease modelling, regenerative medicine, and personalized medicine, while considering their prospects and limitations.

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“…The fabrication of systems and devices that allow HSE perfusion culture is one of the trending directions in skin-on-achip research. Published data show that dynamic culture prolongs the viability of basal layer keratinocytes and improves epidermal morphogenesis and barrier function [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Culture insert device with perfusable microchannels enhances in vitro skin model development and barrier function assessment

Mikimoto,

Mori,

Toyoda

et al. 2024

Biofabrication

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The ever-stricter regulations on animal experiments in the field of cosmetic testing have prompted a surge in skin-related research with a special focus on recapitulation of the in vivo skin structure in vitro. In vitro human skin models are seen as an important tool for skin research, which in recent years attracted a lot of attention and effort, with researchers moving from the simplest 2-layered models (dermis with epidermis) to models that incorporate other vital skin structures such as hypodermis, vascular structures, and skin appendages. In this study, we designed a microfluidic device with a reverse flange-shaped anchor that allows culturing of an in vitro skin model in a conventional 6-well plate and assessing its barrier function without transferring the skin model to another device or using additional contraptions. Perfusion of the skin model through vascular-like channels improved the morphogenesis of the epidermis compared with skin models cultured under static conditions. This also allowed us to assess the percutaneous penetration of the tested caffeine permeation and vascular absorption, which is one of the key metrics for systemic drug exposure evaluation.

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Engineering edgeless human skin with enhanced biomechanical properties

Cited by 8 publications

References 59 publications

Vascularized 3D Human Skin Models in the Forefront of Dermatological Research

Vascularized 3D Human Skin Models in the Forefront of Dermatological Research

Bioengineered skin organoids: from development to applications

Culture insert device with perfusable microchannels enhances in vitro skin model development and barrier function assessment

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