Quantitative estimation of heterocyclic aromatic amines by ion-exchange chromatography and electrochemical detection

Dyck, Martina; Rollmann, Bruno; Meester, Conrad De

doi:10.1016/0021-9673(94)00837-y

Cited by 33 publications

(22 citation statements)

References 17 publications

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“…The reproducibility of the overall method was calculated from samples processed in quadruplicate. The precision of the results for determination of IQ and MeIQx were consistent with those previously reported by Van Dyck et al [5] and Wu et al [10]. No previous reference was found to the determination of 4,8-DiMeIQx.…”

Section: Analytical Applicationssupporting

confidence: 86%

“…These compounds can induce the growth of tumors in the liver, lung, breast, small and large intestine, and other sites in rodents and might also be human carcinogens [1][2][3][4]. They are formed during cooking and flavor-processing of protein-rich foods such as meat and fish by condensation of amino acids, sugar and creatinine or creatine in a Maillard-type reaction [5,6]. Frying and boiling generate the highest concentrations of the compounds, which can reach ng g-1 levels in meat [1].…”

Section: Introductionmentioning

confidence: 99%

“…This method involves the extraction of amines on an Extrelut column, solid-phase extraction (SPE) with clean-up on a strong cation-exchange resin (PRS), and reversedphase (C18) HPLC [7,15]. Several modes of detection were used and sensitivity and selectivity was variable [4][5][6]. This is the currently accepted method for the extraction of this type of amine; recoveries for PHIP, however, vary markedly between applications [2,7,14].…”

Section: Introductionmentioning

confidence: 99%

“…There are several methods for determining genotoxic and mutagenic compounds. Liquid chromatography with ultraviolet, electrochemical, or fluorescence detection, liquid chromatography and gas chromatography coupled with mass spectrometry, enzyme immunoassay [5,8,9] and capillary electrophoresis (CE) [10][11][12] have recently been used for this purpose. The last technique is characterized by high separation efficiency, high selectivity, excellent sensitivity, easy automation, and low operating costs [12,13].…”

Section: Introductionmentioning

confidence: 99%

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Determination of heterocyclic aromatic amines in fried beefsteak, meat extract, and fish by capillary zone electrophoresis

et al. 1998

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SummaryA capillary zone electrophoretic (CZE) method has been developed and optimized for the simultaneous determination of 2-amino- (4,, and 2-amino-l-methyl-6-phenylimidazo [4,5-b]pyridine (PHIP) in fried beefsteak, meat extract, and baked salmon. Solid-phase extraction (the Gross methodology) was tested for isolating the amines. Under optimum capillary electrophoresis conditions the proposed method separated heterocyclic aromatic amines (HAAs) within 20 min. Diode-array UV photometric detection was used and peak identities were confirmed by comparison of spectra. The limits of detection of the method were between 0.05 and 0.22 ~g mL -1 with hydrodynamic injection, and from 0.08 to 0.16 gg mL -1 with electrokinetic injection; injection reproducibility (RSD %) was better with pressure injection (4.7-7.9 %) than with electrokinetic injection (8.6-20.7 %). The method was applied to the analysis of real and spiked samples; recoveries ranged from 6 % for PHIP to 91% for 4,8-DiMeIQx from various cooked foods.

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Section: Analytical Applicationssupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of heterocyclic aromatic amines in fried beefsteak, meat extract, and fish by capillary zone electrophoresis

et al. 1998

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“…Different separation and detection methods for HAAs include GC-MS [18] and HPLC-DAD [21,24], HPLC-FD [15,22,[25][26], electrochemical (HPLC-ED) [27,28] or HPLC-MS [14,16,[29][30]. Regarding CE, rapid and higher separation efficiencies using lower amounts of samples are obtained.…”

Section: Introductionmentioning

confidence: 98%

Simultaneous determination of six non‐polar heterocyclic amines in meat samples by supercritical fluid extraction–capillary electrophoresis under fluorimetric detection

et al. 2010

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A novel, sensitive and selective method for the separation and quantification of a group of non-polar heterocyclic amines (9H-pyrido-[3,4-b] indole, norharmane; 1-methyl-9H-pyrido-[3,4-b] indole, harmane; 2-amino-9H-pyrido-[2,3-b] indole, AalphaC; 2-amino-3-methyl-9H-pyrido-[2,3-b] indole, MeAalphaC; 3-amino-1,4-dimethyl-5H-pyrido-[4,3-b] indole, Trp-P-1 and 3-amino-1-methyl-5H-pyrido-[4,3-b] indole, Trp-P-2) in commercial meat samples has been developed. This methodology is faster than others previously described. The method is based on the combination of a supercritical fluid extraction procedure, followed by the analysis of the extracted plug by CE with fluorescence detection. The supercritical fluid extraction procedure was optimized for the clean-up of the samples and the extraction of the analytes. For the electrophoretic separation, the effect of composition, pH and concentration of buffer, organic modifier content, pressure and time of injection, capillary temperature and voltage applied were studied. A 10 mmol/L formic acid-ammonium formate-ACN (10%, v/v) solution at pH 1.5 was selected as the running electrolyte. With 5-s hydrodynamic injection, linear responses in the range from 100 to 1000 ng/mL and detection limits ranging from 15.9 to 28.1 ng/mL were obtained for different amines in less than 13 min. ACN-water (1:1 in volume) was used as a sample solvent. Fluorescence detection enhances the sensitivity and avoids interferences coming from non-fluorescent compounds present in the matrices of the sample extracts.

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Analytical Aspects of Aromatic Amines

Zabicky

2009

Patai's Chemistry of Functional Groups

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Quantitative estimation of heterocyclic aromatic amines by ion-exchange chromatography and electrochemical detection

Cited by 33 publications

References 17 publications

Determination of heterocyclic aromatic amines in fried beefsteak, meat extract, and fish by capillary zone electrophoresis

Determination of heterocyclic aromatic amines in fried beefsteak, meat extract, and fish by capillary zone electrophoresis

Simultaneous determination of six non‐polar heterocyclic amines in meat samples by supercritical fluid extraction–capillary electrophoresis under fluorimetric detection

Analytical Aspects of Aromatic Amines

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