Quantitative determination of maitenin and 22β-hydroxymaitenin in callus ofMaytenus aquifolium (celastraceae) by reverse phase high performance liquid chromatography

Corsino, Joaquim; Alécio, Alberto Camilo; Ribeiro, Maria Lúcia; Furlan, Maysa; Pereira, Ana Maria Soares; Duarte, Ines B.; França, Suzelei C.

doi:10.1002/(sici)1099-1565(199809/10)9:5<245::aid-pca413>3.0.co;2-t

Cited by 17 publications

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“…The analytical method described herein proved suitable for the analysis of leaf extracts, and is therefore considered more appropriate for analyzing "espinheira santa" phytomedicines than other procedures previously reported. 10,11 …”

Section: Discussionmentioning

confidence: 99%

“…2 However, reports in the literature on quinonemethide triterpenes (displaying cytotoxic activity and therefore containing potentially toxic compounds) in Maytenus samples suggest that "espinheira santa"-based phytomedicines must be carefully investigated. These compounds have been quantified by HPLC-UV external standard in cell cultures (callus extracts) of Maytenus aquifolium, 10 and HPLC quantification of quinonemethide triterpenes in the root bark of Maytenus ilicifolia (another species known as "espinheira santa") has also been reported. 11 However, these HPLC procedures offer a very limited assessment of the safety of Maytenus-based phytomedicines, since they are based mainly on hydroalcoholic or aqueous leaf extracts, 2,12 which contain interfering compounds different from those found in less polar root or callus extracts.…”

Section: Introductionmentioning

confidence: 99%

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HPLC-UV and LC-MS analysis of quinonemethides triterpenes in hydroalcoholic extracts of "espinheira santa" (Maytenus aquifolium Martius, Celastraceae) leaves

Nossack

Celeghini

Lanças

et al. 2004

J. Braz. Chem. Soc.

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Alguns triterpenos quinonametídicos são conhecidos pela sua citotoxicidade, havendo relatos destas substâncias em várias espécies do gênero Maytenus (Celastraceae), inclusive em Maytenus aquifolium Martius, uma das espécies conhecidas no Brasil como "espinheira santa". Com o objetivo de dispor de um método analítico útil na avaliação da qualidade de medicamentos fitoterápicos de "espinheira santa", foi desenvolvido um procedimento de análise por cromatografia líquida de alta eficiência com detector ultravioleta (CLAE-UV). Foi realizada a quantificação dos triterpenos quinonametídicos maitenina e pristimerina, nos extratos hidroalcoólico e aquoso ("chá") das folhas e das cascas das raízes de M. aquifolium. As análises por cromatografia líquida acoplada à espectrometria de massas (LC-MS) usando a interface de ionização química à pressão atmosférica (APCI), confirmaram a presença de maitenina e pristimerina nos extratos hidroalcoólicos de M. aquifolium, enquanto que nos extratos aquosos estes triterpenos estão abaixo do limite de detecção.Some quinonemethide triterpenes are known for their cytotoxicity, and the presence of these compounds has been reported in several Maytenus (Celastraceae) species, including Maytenus aquifolium Martius, one of the species known in Brazil as "espinheira santa". A quantitative HPLC-UV procedure was therefore developed with the purpose of devising a useful analytical method for evaluating the quality of "espinheira santa"-based phytomedicines. Quinonemethide triterpenes pristimerin and maitenin were quantified in hydroalcoholic and aqueous extracts ("teas") prepared from the leaves and root bark of M. aquifolium. Analyses by liquid chromatography coupled to mass spectrometry (LC-MS), using the atmospheric pressure chemical ionization (APCI) interface, provided unequivocal confirmation of the presence of maitenin and pristimerin in M. aquifolium hydroalcoholic extracts, although these compounds were below detection limits in aqueous extracts. Keywords: Maytenus aquifolium Martius, Celastraceae, quinonemethide triterpenes, HPLC-UV, LC-MS IntroductionMaytenus aquifolium Martius and M. ilicifolia Martius (Celastraceae) are both known in Brazil as "espinheira santa", and systematic studies are focusing on their use as phytomedicines for the treatment of gastric ulcers and gastritis.1-3 Reports on studies of Maytenus aquifolium include pharmacology, 4 phytochemistry of flavonoids 5 and sesquiterpene 6 pyridine alkaloids biosynthesis studies 7 and authentication of phytomedicines (leaf samples) by chromatographic analysis (GC, 8 HPTLC 9 ). Use of any medicinal plant as a phytomedicine requires the prior evaluation of its efficacy and safety. Toxicological essays of Maytenus aquifolium leaf extracts spray-dried with 70% ethanol have indicated no significant toxicity in mice. 2 However, reports in the literature on quinonemethide triterpenes (displaying cytotoxic activity and therefore containing potentially toxic compounds) in Maytenus samples suggest that "espinheira santa"-based phyt...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

HPLC-UV and LC-MS analysis of quinonemethides triterpenes in hydroalcoholic extracts of "espinheira santa" (Maytenus aquifolium Martius, Celastraceae) leaves

Nossack

Celeghini

Lanças

et al. 2004

J. Braz. Chem. Soc.

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“…[25] with a few modifications. The samples were analyzed on an HPLC system from Shimadzu (Kyoto, Japan), namely, an LC-10-AVP instrument equipped with an autoinjector SIL-10AF, a photodiode array detector SPD-M20A, and a Phenomenex (Torrance, CA, USA) Luna C 18 column (250 × 4.6 mm i.d.…”

Section: Methodsmentioning

confidence: 99%

Production of the Quinone-Methide Triterpene Maytenin byIn VitroAdventitious Roots ofPeritassa campestris(Cambess.) A.C.Sm. (Celastraceae) and Rapid Detection and Identification by APCI-IT-MS/MS

Paz

Santos

Inácio

et al. 2013

BioMed Research International

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Establishment of adventitious root cultures of Peritassa campestris (Celastraceae) was achieved from seed cotyledons cultured in semisolid Woody Plant Medium (WPM) supplemented with 2% sucrose, 0.01% PVP, and 4.0 mg L−1 IBA. Culture period on accumulation of biomass and quinone-methide triterpene maytenin in adventitious root were investigated. The accumulation of maytenin in these roots was compared with its accumulation in the roots of seedlings grown in a greenhouse (one year old). A rapid detection and identification of maytenin by direct injection into an atmospheric-pressure chemical ionization ion trap tandem mass spectrometer (APCI-IT-MS/MS) were performed without prior chromatographic separation. In vitro, the greatest accumulation of biomass occurred within 60 days of culture. The highest level of maytenin—972.11 μg·g−1 dry weight—was detected at seven days of cultivation; this value was 5.55-fold higher than that found in the roots of seedlings grown in a greenhouse.

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“…9,10,11,12,13,6 The genus Maytenus, of Celastraceae, was selected among 200 species as a putative matrix to develop the in vitro system with the objective of improving the production and to obtain a useful model system for studying the biosynthesis of these valuable compounds. 14,8,15 We closely monitored growth parameters such as length of lag, of log phases, and growth speed during the log phase, for each of the different callus lines obtained from Maytenus ilicifolia (Celastraceae) and their capacity for producing quinonemethides. Cell tissues growth curve demonstrated that the high yielding of the compounds was at the logarithmic phase.…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation of Maytenus ilicifolia (Celastraceae) as potential source for antitumoral and antioxidant quinomethide triterpenes production. A rapid quantitative method for their analysis by reverse-phase high-performance liquid chromatography

Filho¹,

Bolzani²,

Furlan³

et al. 2004

Arkivoc

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Cell culture of Maytenus ilicifolia were established in order to produce and to quantify the antitumoral and antioxidant quinonemethide triterpenes. In vitro calli were induced from leaf explants of native plants and cultured in semi-solid medium under controlled conditions of humidity, temperature and photoperiod. The quinonemethide triterpenes showed maximum accumulation in the logarithmic phase growth of the cell culture. A rapid, sensitive and reliable reverse-phase HPLC method was used for quantitative determination of the antitumoral and antioxidant quinonemethide triterpenes, 22β-hydroxymaytenin and maytenin in callus of Maytenus ilicifolia. Well resolved peaks with good detection response and linearity in the range 1.0 -100 µg/mL were obtained. This quantitative work was performed by an external standard method.

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Quantitative determination of maitenin and 22β-hydroxymaitenin in callus ofMaytenus aquifolium (celastraceae) by reverse phase high performance liquid chromatography

Cited by 17 publications

References 0 publications

HPLC-UV and LC-MS analysis of quinonemethides triterpenes in hydroalcoholic extracts of "espinheira santa" (Maytenus aquifolium Martius, Celastraceae) leaves

HPLC-UV and LC-MS analysis of quinonemethides triterpenes in hydroalcoholic extracts of "espinheira santa" (Maytenus aquifolium Martius, Celastraceae) leaves

Production of the Quinone-Methide Triterpene Maytenin byIn VitroAdventitious Roots ofPeritassa campestris(Cambess.) A.C.Sm. (Celastraceae) and Rapid Detection and Identification by APCI-IT-MS/MS

In vitro propagation of Maytenus ilicifolia (Celastraceae) as potential source for antitumoral and antioxidant quinomethide triterpenes production. A rapid quantitative method for their analysis by reverse-phase high-performance liquid chromatography

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