Quantitative determination of ceftiofur-related residues in bovine raw milk by LC-MS/MS with electrospray ionization

Pharmacokinetic profile of Ceftiofur Hydrochloride Injection in lactating Holstein dairy cows

“…The concentrations of ceftiofur and desfuroylceftiofur‐related metabolites (DFC powders were purchased from Sigma corporation) in plasma were measured using a modification validated ultra‐high‐pressure liquid chromatography mass/mass spectrometer (UPLC‐MS/MS) assay for desfuroylceftiofur acetamide (DCA) as previously described (Becker, Zittlau, & Petz, ). This UPLC‐MS/MS‐DCA method is based on the cleavage of the disulfide and/or thioester bonds between the metabolites and their conjugate sulfur containing moiety using DTE to yield desfuroylceftiofur, derivatization of DFC to the more stable derivative DCA and concentration.…”

Quechers methodologies as an alternative to solid phase extraction (SPE) for the determination and characterization of residues of cephalosporins in beef muscle using LC–MS/MS

“…MeCN was chosen as the organic solvent because its good miscibility with water, and its ability to recover the analytes without extracting high quantities of lipophilic material [21]. As it can be found in the literature, MeCN is one of the most popular solvents for the extraction of ␤-lactams [11,16,17,31]. Water was selected as the other extraction solvent to facilitate the extraction of the most polar components.…”

Rapid and simultaneous determination of amoxicillin, penicillin G, and their major metabolites in bovine milk by ultra-high-performance liquid chromatography–tandem mass spectrometry

“…In literature, different volumes of organic solvents, including acetonitrile [14,21,29,32,33,[37][38][39][40] and ethyl acetate [41] were used for deproteinising milk samples. Others using borate buffer with DTE [42] or TCA [43] have also been reported. Almost all their sample pre-treatments included the time-consuming step of solid phase extraction (SPE), which hampered the analysis of many samples within one day.…”