Quantitative determination of acid-labile dna in cervical intraepithelial neoplasia

Abstract

Using a modified Feulgen hydrolysis procedure and integrating microdensitometry, the acid-labile nuclear DNA in exfoliated cervical epithelial cells was quantified in a range of histologically confirmed cervical intraepithelial neoplasia (CIN), invasive cancer, and normal controls. The mean relative optical densities obtained for each sample group showed an increase from normal epithelium, through CIN grades, to invasive cancer. Although there was some overlap between groups, the difference in the overall mean values between the adjacent groups was statistically significant. The sensitivity of the test was 87.1% with a specificity of 99.2% and a predictive value of 99.5%, with no false negatives in the severe dysplasia and cancer groups. Quantitative data allows the threshold value to be altered to vary the sensitivity and specificity according to prevailing requirements. This suggests the possibility of using quantitative acid-labile DNA measurements to improve existing screening for cervical precancer. Cancer 67:3104-3109,1991. HE TRADITIONAL PAPANICOLAOU method of Cyto

Quantitative assessment of bladder carcinoma by acid labile DNA assay

“…Looking at the hydrolysis curves in our cases, it becomes clear that the increase of hydrolyzed DNA in nuclei from benign urothelium is delayed compared with that of malignant tumor cell nuclei. This has been reported to be a constant finding in various tissues investigated 13–19. In our cases, this early increase was even more pronounced in the more aggressive tumors ( P = 0.001; data not shown).…”

“…In contrast to determining DNA ploidy, other studies have investigated the dynamics of the Feulgen hydrolysis reaction, which differentiates a rapidly hydrolyzed component of nuclear DNA, the increase of which is linked to malignancy 13–15. Semiautomated, quantitative determination of acid‐labile DNA has been applied successfully applied to cervical smears to detect cervical intraepithelial neoplasia 16–19. We measured the rapidly hydrolyzable fraction of DNA from patients with normal urothelium and with urothelial tumors to determine whether sufficient discrimination can be achieved between these groups for the purpose of monitoring patients with carcinoma of the bladder and bladder carcinoma screening.…”

Use of Nuclear Morphometry Characteristics to Distinguish between Normal and Abnormal Cervical Glandular Histologies

“…There is evidence that QP methods have potential in detecting malignancy-associated changes -premalignant lesions associated with changes in the DNA structure which cannot be detected using classical visual tech-niques [17]. QP has also successfully monitored regression/progression in chemoprevention studies examining premalignant conditions [3,23], and the measurements can be correlated to biomarkers evaluating ploidy [20,21].…”

Cytometric features of cell nuclei of adenocarcinoma in situ and invasive adenocarcinoma of the cervix

“…15 Such nuclear features may be related to the ploidy of the cell and the degree of abnormality, allowing a quantification of the degree of abnormality that can be correlated with other biomarkers to evaluate ploidy. 16,17 Certain nuclear features have proved useful in other tissue models to discriminate between normal, premalignant, and malignant cell types. These methods may also have the potential to detect premalignant lesions before DNA changes can be detected by classic visual techniques.…”