Use of Nuclear Morphometry Characteristics to Distinguish between Normal and Abnormal Cervical Glandular Histologies

Swartz, Richard J.; West, Loyd A.; Boiko, Iouri; Malpica, Anaís; MacAulay, Calum; Carraro, Anita; Guillaud, Martial; Cox, Dennis D.; Follen, Michele

doi:10.1155/2003/207481

Cited by 9 publications

(5 citation statements)

References 21 publications

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“…Moreover, our results demonstrate that QDP can detect serial nuclear changes in the sequential progression of BE from normal epithelium to dysplastic epithelium to frank INV. We previously observed similar findings in multiple human epithelium sites, including the oral cavity, 21 lung, 32 cervix, 36 and breast, 37 while other independent groups have reported similar evidence in skin, 38 colon, 39 endometrium, 40 and ovary. 41 We have then showed that measurement of nuclear chromatin texture is significant because such changes are an indication of genetic or epigenetic changes that lead toward malignant transformation.…”

Section: Discussionsupporting

confidence: 76%

Evaluation of Quantitative Digital Pathology in the Assessment of Barrett Esophagus–Associated Dysplasia

Guillaud¹,

Korbelik²,

Marginean³

2015

Am J Clin Pathol

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Section: Discussionsupporting

confidence: 76%

Evaluation of Quantitative Digital Pathology in the Assessment of Barrett Esophagus–Associated Dysplasia

Guillaud¹,

Korbelik²,

Marginean³

2015

Am J Clin Pathol

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“…Nuclear morphometry has been found to be useful in squamous cell epithelial abnormality as well as glandular cell abnormality of cervix. [ 10 12 17 18 19 ] In the indexed study on morphometry of squamous epithelial cell abnormality, the nuclear parameters like area, perimeter, and diameter were found to be significant in differentiating NILM or reactive cellular changes associated with inflammation from intraepithelial lesions and carcinomas. Also, nuclear morphometry is helpful in differentiating between the groups.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the cytonucleomorphometric parameters for cases diagnosed as squamous cell abnormality on conventional cervico-vaginal pap smears

Khurana¹,

Mishra²,

Kapoor³

et al. 2023

J Cytol

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Background and Aim: The natural history of cervical cancer is unique that it is preceded by a precancerous condition for a long time. Morphometry as a tool can be used in early and accurate diagnosis of these precancerous and cancerous lesions. The present study aims at assessing the utility of cellular and nuclear morphometry in differentiating squamous cell abnormality from benign conditions and also differentiating the categories of squamous cell abnormalities. Materials and Methods: Forty-eight diagnosed cases of squamous epithelial cell abnormality, that is, 10 cases each of atypical squamous cell of undetermined significance (ASC-US), low-grade squamous intraepithelial lesion (LSIL), high-grade SIL (HSIL), and squamous cell carcinoma (SCC) and eight cases of ASC-H (ASC cannot exclude HSIL), were made the sample population and compared with a control population of 10 cases of negative for intraepithelial lesion or malignancy (NILM). Parameters like nuclear area (NA), nuclear perimeter (NP), nuclear diameter (ND), nuclear compactness (NC), cellular area (CA), cellular diameter (CD), cellular perimeter (CP), and nucleocytoplasmic (N/C) ratio were used. Results: There was a significant difference in the six groups of squamous cell abnormality based on NA, NP, ND, CA, CP, and CD ( P < 0.05) using one-way analysis of variance. Nuclear morphometry parameters like NA, NP, and ND were found to be the maximum for HSIL, followed by LSIL, ASC-H, ASC-US, SCC, and NILM groups in decreasing order. The mean CA, CP, and CD were found to be the maximum for NILM, followed by LSIL, ASC-US, HSIL, ASC-H, and SCC in decreasing order. On post hoc analysis, the lesions can be divided into three groups: NILM/normal; ASC-US and LSIL; and ASC-H, HSIL, and SCC, based on N/C ratio. Conclusion: In cervical lesions, holistic parameter of cytonucleomorphometry should be taken rather than taking nuclear morphometry only. N/C ratio is a highly statistically significant parameter that can differentiate between low-grade lesions and high-grade lesions.

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“…Studies of cell volume variations are very important because these data under normal conditions appear to be constant for different cell types independent of body size. The mass of a body depends on the number rather than the volume of cells [21]. It is also known that changes in nuclear volume reflect the functional status of cells.…”

Section: Discussionmentioning

confidence: 99%

“…When cells are in full physiological activity the nuclear volume is higher than when they are at rest [22]. This is probably the result of changes induced by the synthesis of DNA, RNA or proteins related to the cell maintenance process [21,22].…”

Section: Discussionmentioning

confidence: 99%

Protective effect of annexin‐A1 against irreversible damage to cavernous tissue after cavernous nerve injury in the rat

Junior

Burnett²

2012

BJU International

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Study Type – Aetiology (case control) Level of Evidence 3b What's known on the subject? and What does the study add? Penile rehabilitation is still controversial regarding good results. Our study shows a non‐invasive treatment option to recovery after cavernous nervous damage. The assessment of changes in the intracavernous pressure and karyometry demonstrates the protective effect of annexin‐A1 in an animal model of cavernous nerve injury. We found that annexin‐A1 effectively preserved erectile function, evidently through significantly protecting the corpus cavernosum tissue against fibrosis. OBJECTIVE To evaluate the protective effect of annexin‐A1 against irreversible damage to cavernous tissue after cavernous nerve injury. PATIENTS AND METHODS Thirty Sprague‐Dawley male rats were divided into 3 groups; sham‐operated rats (n= 10), bilateral cavernous nerve injury treated intravenously with 100 µg/kg annexin‐A1 (n= 10), and a crush group of rats submitted to bilateral cavernous nerve injury and vehicle (n= 10). Groups were compared in respect to intracavernous pressure and karyometric parameters. RESULTS After annexin‐A1 treatment, the maximum changes in intracavernous pressure responses were significantly higher in the annexin‐A1 group compared to the vehicle‐only group on the 7th postoperative day (p‐value <0.05). Hematoxylin‐eosin staining showed that the percentage of cavernosal smooth muscle was higher in the annexin‐A1 group. Karyometry showed that the nuclear volume was greater in the annexin‐A1 group, as was the major/minor smooth muscle cell diameter ratio compared to the vehicle‐only group on the 7th postoperative day (p‐value <0.05). CONCLUSION This is the first report that, by assessing changes in the intracavernous pressure and karyometry, demonstrates the protective effect of annexin‐A1 in an animal model of cavernous nerve injury. We found that annexin‐A1 effectively preserved erectile function, evidently through significantly protecting the corpus cavernosum tissue against fibrosis.

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Use of Nuclear Morphometry Characteristics to Distinguish between Normal and Abnormal Cervical Glandular Histologies

Cited by 9 publications

References 21 publications

Evaluation of Quantitative Digital Pathology in the Assessment of Barrett Esophagus–Associated Dysplasia

Evaluation of Quantitative Digital Pathology in the Assessment of Barrett Esophagus–Associated Dysplasia

Evaluation of the cytonucleomorphometric parameters for cases diagnosed as squamous cell abnormality on conventional cervico-vaginal pap smears

Protective effect of annexin‐A1 against irreversible damage to cavernous tissue after cavernous nerve injury in the rat

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