Quantitative characterization of uterine vascular permeability changes with estrogen

Hc, Cecil; Ja, Hannum; Bitman, Joel

doi:10.1152/ajplegacy.1966.211.5.1099

Cited by 23 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One of the early events caused by estradiol is the increased vascular permeability and oedema in the endometrium [47]. This has been associated with the rapid induction of VEGF mRNA expression following administration of E2 in immature and OVX rats [48,49].…”

Section: Discussionmentioning

confidence: 99%

Comparative study of the short-term effects of a novel selective estrogen receptor modulator, ospemifene, and raloxifene and tamoxifen on rat uterus

Zheng

Kangas²,

Härkönen

2004

The Journal of Steroid Biochemistry and Molecular Biology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Comparative study of the short-term effects of a novel selective estrogen receptor modulator, ospemifene, and raloxifene and tamoxifen on rat uterus

Zheng

Kangas²,

Härkönen

2004

The Journal of Steroid Biochemistry and Molecular Biology

View full text Add to dashboard Cite

“…This increase in blood flow may explain why the estrogen plus VEGF antiserum-treated rat uteri tended to be slightly larger than the control uteri (although the difference was not statistically significant) and may also explain the very faint and diffuse blue staining of the uteri of mated, anti-VEGFtreated mice even though no implantation occurred. Increased uterine blood flow would both increase intravascular blood volume (2.5-to 2.7-fold) [45,46] and raise capillary hydrostatic pressure. The latter would be predicted to increase net filtration without any change in permeability, perhaps resulting in a modest increase in steady-state interstitial fluid volume.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Vascular Endothelial Growth Factor/Vascular Permeability Factor Action Blocks Estrogen-Induced Uterine Edema and Implantation in Rodents1

Rockwell

Pillai

Olson

et al. 2002

Biology of Reproduction

113

View full text Add to dashboard Cite

Estrogen induces a rapid increase in microvascular permeability in the rodent uterus, leading to stromal edema and a marked increase in uterine wet weight. This edema is believed to create an environment optimal for the growth and remodeling of the endometrium in preparation for implantation and pregnancy. Increased endometrial microvascular permeability also occurs in conjunction with implantation. Estrogen-induced uterine edema is immediately preceded by an increase in the expression of vascular endothelial growth factor (VEGF), a potent stimulator of microvascular permeability. The objective of this study was to determine to what degree immunoneutralization of VEGF would interfere with a) estradiol-induced uterine edema and b) pregnancy. In the first set of experiments, immature female rats were injected with either VEGF antiserum or normal rabbit serum (NRS) prior to 17beta-estradiol treatment. Rats treated with estradiol alone showed a 57% increase in uterine wet weight at 6 h compared with controls. Injection of 200 or 300 micro l of VEGF antiserum reduced the response to only 20% and 10% above controls, respectively. In the second set of experiments, young adult female mice were treated with 100 micro l of either VEGF antiserum or NRS at 1200 h on the fourth day after mating. NRS-treated mice had normal pregnancies. VEGF antiserum, however, completely blocked pregnancy. When VEGF antiserum-treated females were examined on Day 5 for the presence of implantation sites, none were found. These results show that a) VEGF is the major mediator of estrogen-induced increase in uterine vascular permeability and b) VEGF-induced edema is absolutely essential for implantation to take place.

show abstract

“…30 min. before killing (Judah & Willoughby, 1962;Cecil, Hannum & Bitman, 1966). A comparable group of rats that were only pseudopregnant served as controls.…”

Section: Methodsmentioning

confidence: 99%

Uterine Deciduoma Induction: Comparison of Direct Mechanical Trauma With Systemic Pyrathiazine

Wrenn¹,

Bitman²,

Wood³

1967

Journal of Endocrinology

View full text Add to dashboard Cite

Uterine deciduoma induction in rats by the systemic administration of the antihistamine, pyrathiazine, was compared with induction by intraluminal scratching. Intraperitoneal injection of pyrathiazine hydrochloride (25 mg.) was effective as a deciduoma inducer in 52 % of the animals.Intrauterine scratching resulted in a higher water content of the uterus than pyrathiazine treatment. Differences in histamine concentration of the uteri were not significant, but there were differences in tissue permeability, determined by the uptake of trypan blue. Tissue permeability was measured \ m=1/ 2\ , 1\ m=1/ 2\ and 4 hr. after deciduoma induction. A diphasic pattern of increased tissue permeability was observed in the pyrathiazine-treated rats which duplicated the characteristic response of inflammation and injury, suggesting that systemically given pyrathiazine evoked the decidual reaction as a result of injury to the uterus.

show abstract

Quantitative characterization of uterine vascular permeability changes with estrogen

Cited by 23 publications

References 0 publications

Comparative study of the short-term effects of a novel selective estrogen receptor modulator, ospemifene, and raloxifene and tamoxifen on rat uterus

Comparative study of the short-term effects of a novel selective estrogen receptor modulator, ospemifene, and raloxifene and tamoxifen on rat uterus

Inhibition of Vascular Endothelial Growth Factor/Vascular Permeability Factor Action Blocks Estrogen-Induced Uterine Edema and Implantation in Rodents1

Uterine Deciduoma Induction: Comparison of Direct Mechanical Trauma With Systemic Pyrathiazine

Contact Info

Product

Resources

About