Quantitative analysis of the dystrophin gene by real-time PCR

Maksimović, Nela; Andjelković, Ana; Milic-Rasic, Vedrana; Rakočević-Stojanović, Vidosava; Kastratović-Kotlica, Biljana; Brankovic, Slavko; Damnjanovic, Tatjana; Jekic, Biljana; Bunjevacki, Vera; Lukovic, Ljiljana; Perovic, Dijana; Cvjetićanin, Suzana; Novaković, Ivana

doi:10.2298/abs1202787m

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…Eight GbEXPs in four subgroups, including GbEXPA31 , were quantified by qRT-PCR on an Applied Biosystems ® 7500 Real Time PCR System as the instruction of MonAmp™ SYBR ® Green qPCR Mix (Monad, Nanjing, China) with specific primers ( Supplementary Table S2 ); the reaction program in PCR was fully followed as the introduction of kit. Ginkgo GAPDH was utilized as a housekeeping gene to determine the relative expression with the 2 −ΔΔCT method [ 61 ].…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification of Expansin Gene Family and Their Response under Hormone Exposure in Ginkgo biloba L.

Guo

El‐Kassaby

et al. 2023

IJMS

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Expansins are pH-dependent enzymatic proteins that irreversibly and continuously facilitate cell-wall loosening and extension. The identification and comprehensive analysis of Ginkgo biloba expansins (GbEXPs) are still lacking. Here, we identified and investigated 46 GbEXPs in Ginkgo biloba. All GbEXPs were grouped into four subgroups based on phylogeny. GbEXPA31 was cloned and subjected to a subcellular localization assay to verify our identification. The conserved motifs, gene organization, cis-elements, and Gene Ontology (GO) annotation were predicted to better understand the functional characteristics of GbEXPs. The collinearity test indicated segmental duplication dominated the expansion of the GbEXPA subgroup, and seven paralogous pairs underwent strong positive selection during expansion. A majority of GbEXPAs were mainly expressed in developing Ginkgo kernels or fruits in transcriptome and real-time quantitative PCR (qRT-PCR). Furthermore, GbEXLA4, GbEXLA5, GbEXPA5, GbEXPA6, GbEXPA8, and GbEXPA24 were inhibited under the exposure of abiotic stresses (UV-B and drought) and plant hormones (ABA, SA, and BR). In general, this study expanded our understanding for expansins in Ginkgo tissues’ growth and development and provided a new basis for studying GbEXPs in response to exogenous phytohormones.

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Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification of Expansin Gene Family and Their Response under Hormone Exposure in Ginkgo biloba L.

Guo

El‐Kassaby

et al. 2023

IJMS

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“…Moreover, CFTR and DMD have been detected using immunoprecipitation , immunofluorescence , immunocytochemical methods and immunostaining . Other methods such as NMR spectroscopy , Flow Cytometry , multiplexed and real‐time PCR have been also reported. These methods are highly sensitive and specific, but suffer from the lack of simplicity and time‐consuming or rely on expensive instruments and skilful operators .…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Immunosensors for the Rapid Screening of Cystic Fibrosis and Duchenne Muscular Dystrophy

et al. 2017

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Cystic Fibrosis (CF) and Duchenne Muscular Dystrophy (DMD) are well characterized progressive inherited diseases associated with significant morbidity and mortality. Therefore, the early, rapid and affordable diagnosis of these disorders through newborn screening is highly important for the appropriate management. Here, we report label‐free impedance immunosensors for the simple screening of CF and DMD through the detection of cystic fibrosis transmembrane conductance regulator (CFTR) protein fragment and a peptide sequence for dystrophin (DMD). The biosensors were constructed by the covalent immobilization of specific antibodies for CFTR and DMD on standard gold (Au) electrodes. The immunosensors response was measured based on the change in the electrochemical impedance spectroscopy (EIS) signals after binding with the peptides. The specific recognition of the immunosensor surfaces to the target antigens leads to retardation of the access of ferri‐ferrocyanide redox molecules to the surface and thus, enhances the charge transfer resistance (Rct). These impedimetric immunosensors enabled sensitive, fast, selective and accurate estimation of CFTR and DMD levels within a linear range from 1.0 pg/mL to 1 μg/mL and 1.0 pg/mL to 10 ng/mL with lower detection limits of 0.8 and 0.7 pg/mL for CFTR and DMD, respectively. Moreover, the immunosensors were tested for the detection of CFTR and DMD in human serum showing very good agreement with enzyme‐linked immunosorbent assays (ELISA). This work represents a novel low cost analytical method that aims to satisfy the unmet public health need in the early diagnosis of CF and DMD and can be extended to detect other hereditary disorders.

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Carbon nanofiber-based multiplexed immunosensor for the detection of survival motor neuron 1, cystic fibrosis transmembrane conductance regulator and Duchenne Muscular Dystrophy proteins

Eissa

Alshehri

Abduljabbar³

et al. 2018

Biosensors and Bioelectronics

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Quantitative analysis of the dystrophin gene by real-time PCR

Cited by 3 publications

References 16 publications

Genome-Wide Identification of Expansin Gene Family and Their Response under Hormone Exposure in Ginkgo biloba L.

Genome-Wide Identification of Expansin Gene Family and Their Response under Hormone Exposure in Ginkgo biloba L.

Electrochemical Immunosensors for the Rapid Screening of Cystic Fibrosis and Duchenne Muscular Dystrophy

Carbon nanofiber-based multiplexed immunosensor for the detection of survival motor neuron 1, cystic fibrosis transmembrane conductance regulator and Duchenne Muscular Dystrophy proteins

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