Electrochemical Immunosensors for the Rapid Screening of Cystic Fibrosis and Duchenne Muscular Dystrophy

Alshehri, Nawal; Eissa, Shimaa; Balobaid, Laila; Rahman, Anas M. Abdel; Dasouki, Majed; Zourob, Mohammed

doi:10.1002/elan.201700155

Cited by 8 publications

(3 citation statements)

References 22 publications

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“…After the incubation with cysteamine (red curve), an increase in the peak current and a decrease in the ΔE was observed indicating the success of the self assembly of the cysteamine on the electrode surface. The terminal amine groups of the cysteamine show a positive charge which attracts the redox anions from the solution causing enhancement of the electron transfer rate .…”

Section: Resultsmentioning

confidence: 99%

Voltammetric Label‐free Immunosensors for the Diagnosis of Cystic Echinococcosis

2020

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Cystic echinococcosis (CE) or hydatid disease is a parasitic infection caused by Echinococcus granulosus. Early serodiagnosis and continuous monitoring of the disease is very important for medical treatment. Here, we report the detecting of both echinococcus antigen and antibody for the diagnosis of hydatid disease using square wave voltammetry (SWV)‐based immunosensors. The gold electrodes were functionalized using cysteamine/phenylene diisothiocyanate linkers and used for the immunosensors fabrication. The hydatid antigen and antibody immunosensors were constructed by the immobilization of either purified rabbit polyclonal antibody or recombinant antigen B (AgB), respectively on the functionalized gold electrodes surfaces. The detection in both cases was achieved by following the change in the SWV reduction peak current of the ferro/ferricyanide redox couple upon antibody or antigen binding. These immunosensors enabled the detection of echinococcus antigen and antibody within a concentration range of 1 pg.mL−1 to 1 μg.mL−1 with detection limits of 0.4 pg.mL−1 and 0.3 pg.mL−1, respectively. A preliminary application of the developed immunosensor was performed in spiked serum sample showing good recovery percentages ranging from 102 to 110 % for both hydatid antibody and antigen detection. This easy‐to‐use, sensitive, and low cost quantitative method holds great promise for the early diagnosis of hydatid disease and thus, better managements and treatment outcomes.

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Section: Resultsmentioning

confidence: 99%

Voltammetric Label‐free Immunosensors for the Diagnosis of Cystic Echinococcosis

2020

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“…While the classical presentation of CF is well known, a definitive CF diagnosis is only achieved by a combination of symptoms, family history, a positive newborn screening test, and laboratory evidence of CFTR malfunction, e.g ., positive genetic testing for two CFTR mutations (one in each parental gene) or elevated sweat sodium chloride levels. ,,− Novel biosensor-based approaches have suggested that the level of the soluble form of CFTR protein may be used as a diagnostic marker for CF in newborns using dried blood spots …”

Section: Introductionmentioning

confidence: 99%

“…3,4,8−11 Novel biosensor-based approaches have suggested that the level of the soluble form of CFTR protein may be used as a diagnostic marker for CF in newborns using dried blood spots. 12 Currently, there is no cure for CF. Therapies that correct CFTR function are promising but still not sufficiently available to patients.…”

Section: Introductionmentioning

confidence: 99%

Lipidome Alterations Induced by Cystic Fibrosis, CFTR Mutation, and Lung Function

Buzatto

Jabar

Nizami³

et al. 2020

J. Proteome Res.

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Cystic fibrosis is a genetic pathology characterized by abnormal accumulation of mucus in the respiratory, gastrointestinal, and reproductive tracts, caused by mutations in the CFTR gene. Although the classical presentation of the condition is well known, there is still a need for a better characterization of metabolic alterations related to cystic fibrosis and different genotypic mutations. We employed untargeted, comprehensive lipidomics of blood serum samples to investigate alterations in the lipid metabolism related to the pathology, mutation classes, and lung function decline. Six unique biomarker candidates were able to independently differentiate diseased individuals from healthy controls with excellent performance. Cystic fibrosis patients showed dyslipidemia for most lipid subclasses, with significantly elevated odd-chain and polyunsaturated fatty acyl lipids. Phosphatidic acids and diacylglycerols were particularly affected by different genotypic mutation classes. We selected a biomarker panel composed of four lipids, including two ceramides, one sphingomyelin, and one fatty acid, which correctly classified all validation samples from classes III and IV. A biomarker panel of five oxidized lipids was further selected to differentiate patients with reduced lung function, measured as predicted FEV1%. Our results indicate that cystic fibrosis is deeply related to lipid metabolism and provide new clues for the investigation of the disease mechanisms and therapeutic targets.

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Development of Impedimetric Immunosensors for the Diagnosis of DOCK8 and STAT3 Related Hyper‐Immunoglobulin E Syndrome

et al. 2018

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Hyper Immunoglobulin E syndrome (HIES) is a rare inherited inborn error of primary immunodeficiency. HIES is characterized by high levels of serum immunoglobulin E (IgE), severe eczema, which overlap with atopic dermatitis, as well as lung infections and even death. HIESs are genetically inherited by heterozygous or homozygous mutations in signal transducer and activator of transcription 3 (STAT3) or Dedicator of cytokinesis 8 (DOCK8) genes, in autosomal dominant and recessive forms, respectively. Therefore, the early detection of DOCK8 and STAT3 protein levels in humans would facilitate the early diagnosis of these disorders and thus, help in the disease management. Here, we present the development of immunosensors for the detection of DOCK8 and STAT3 using electrochemical impedance spectroscopy in a label‐free format. The immunosensors were fabricated by the covalent attachment of specific antibodies for DOCK8 and STAT3 on gold electrodes via cysteamine/phenylene diisothiocyanate linkers. The detection was achieved by monitoring the change in the charge transfer resistance (Rct) of ferro/ferricyanide redox couple upon binding of the proteins to the immunosensor surface. These biosensors enabled the detection of DOCK8 and STAT3 levels with low detection limits of 1.2 and 9.0 pg/ml, respectively. The immunosensor was also applied for the detection of DOCK8 in human serum samples showing high recovery percentages which indicates great promise of this method for early diagnosis of HIES in newborn infants.

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Electrochemical Immunosensors for the Rapid Screening of Cystic Fibrosis and Duchenne Muscular Dystrophy

Cited by 8 publications

References 22 publications

Voltammetric Label‐free Immunosensors for the Diagnosis of Cystic Echinococcosis

Voltammetric Label‐free Immunosensors for the Diagnosis of Cystic Echinococcosis

Lipidome Alterations Induced by Cystic Fibrosis, CFTR Mutation, and Lung Function

Development of Impedimetric Immunosensors for the Diagnosis of DOCK8 and STAT3 Related Hyper‐Immunoglobulin E Syndrome

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