Carbon nanofiber-based multiplexed immunosensor for the detection of survival motor neuron 1, cystic fibrosis transmembrane conductance regulator and Duchenne Muscular Dystrophy proteins

Eissa, Shimaa; Alshehri, Nawal; Abduljabbar, Mai; Rahman, Anas M. Abdel; Dasouki, Majed; Nizami, Imran; Al–Muhaizea, Mohammad A.; Zourob, Mohammed

doi:10.1016/j.bios.2018.05.048

Cited by 19 publications

(6 citation statements)

References 47 publications

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“…Therefore, only two CV cycles were utilized for the electrografting procedure. It is worth noting that the control of the film thickness and surface coverage is crucial in the diazonium electrografting process and thus, it was extensively studied and optimized in our previous work to ensure proper coverage. − …”

Section: Resultsmentioning

confidence: 99%

Combination of Carbon Nanofiber-Based Electrochemical Biosensor and Cotton Fiber: A Device for the Detection of the Middle-East Respiratory Syndrome Coronavirus

2021

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The miniaturization of biosensors for point-of-care diagnosis is highly important in infection control. Electrochemical biosensors offer several advantages in diagnosis in terms of cost, disposability, portability, and sensitivity. Here, a miniaturized electrochemical immunosensor combined with cotton fiber for the detection of the Middle-East respiratory syndrome coronavirus (MERS-CoV) is described. Taking advantage of the absorption capability of cotton, the nasal and saliva samples can be collected and directly transferred to the immunosensor surface for detection using a single tool. The immunosensor was fabricated on a disposable screen-printed electrode precoated with carbon nanofibers. The electrodes were functionalized with carboxyphenyl groups that were used for the immobilization of the spike protein of the MERS-CoV. A competitive detection scheme was employed using the antibody for the MERS-CoV spike protein, and the square-wave voltammetry technique was used for measurements. The biosensor tested after the cotton coating of the electrode exhibited excellent performance. The biosensor was capable of detecting the MERS-CoV spike protein within a concentration range from 0.1 pg•mL −1 to 1 μg•mL −1 with a limit of detection of 0.07 pg•mL, implying the high sensitivity of the method. The immunosensor did not exhibit any cross-reactivity against proteins from HCoV and Influenza A, indicating the excellent selectivity of this approach. Testing of the biosensor in nasal samples showed very high recovery percentages. This disposable biosensor can be used as a miniaturized device for the collection of samples and detection of the virus using a portable potentiostat connected to a smartphone.

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Section: Resultsmentioning

confidence: 99%

Combination of Carbon Nanofiber-Based Electrochemical Biosensor and Cotton Fiber: A Device for the Detection of the Middle-East Respiratory Syndrome Coronavirus

2021

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“…They also used similar AuNP-carbon electrodes to detect DOCK8, PGM3, and STAT3 protein biomarkers with LODs ∼3 pg mL –1 aimed at diagnosis of Hyper-Immunoglobulin E syndrome . They used disposable carbon fiber immunosensors to detect survival motor neuron 1, cystic fibrosis transmembrane conductance regulator, and Duchenne muscular dystrophy proteins …”

Section: Electrochemical Sensingmentioning

confidence: 99%

“…26 They used disposable carbon fiber immunosensors to detect survival motor neuron 1, cystic fibrosis transmembrane conductance regulator, and Duchenne muscular dystrophy proteins. 27 Reports have appeared combining electrochemical immunosensing with a second approach for different kinds of analytes, as well as using novel approaches to electrode materials or detection. Joe Wang's team at UC San Diego developed the first dual-marker electrochemical chip with integrated enzymeand immunodetection.…”

Section: ■ Electrochemical Sensingmentioning

confidence: 99%

Multiplexed Immunosensors and Immunoarrays

et al. 2019

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Immunoassays make use of highly specific antigen-antibody binding and provide sensitive ways to detect a wide range of biomacromolecules, bacteria, viruses, and small molecules. There are a range of types of immunoassay systems including single analyte sensors, 96well plate formats, arrays, microfluidic sensors, microfluidic arrays, etc. A big target is encompassed by medical diagnostic biomarkers, which are "molecules that can be measured objectively as indicators of normal or disease processes and responses to therapeutic intervention". 1 Accurate, low-cost measurements of multiple proteins are major applications of immunoarrays that are critical for future early detection and monitoring of cancer and other diseases. Multiplexing is very important, since panels of biomarker proteins, as opposed to single biomarkers, are required to provide sufficient information content for reliable disease diagnostics.

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“…The high surface area and large number of active sites of CNFs can not only provide the grounds for the adsorption of proteins and enzymes, but CNFs can also provide the direct electron transfer and stabilize enzyme activity [103]. Therefore, CNFs are the most promising substrates for the development of biosensors [104,105,106]. Periyaruppan and coworkers developed a CNF-based nanoelectrode array for cardiac troponin-I (cTnI) detection in the early diagnosis of myocardial infraction [49].…”

Section: Sensor Applications Of Cnf-based Nanomaterialsmentioning

confidence: 99%

Carbon Nanofiber-Based Functional Nanomaterials for Sensor Applications

Wang

et al. 2019

Nanomaterials

117

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Carbon nanofibers (CNFs) exhibit great potentials in the fields of materials science, biomedicine, tissue engineering, catalysis, energy, environmental science, and analytical science due to their unique physical and chemical properties. Usually, CNFs with flat, mesoporous, and porous surfaces can be synthesized by chemical vapor deposition and electrospinning techniques with subsequent chemical treatment. Meanwhile, the surfaces of CNFs are easy to modify with various materials to extend the applications of CNF-based hybrid nanomaterials in multiple fields. In this review, we focus on the design, synthesis, and sensor applications of CNF-based functional nanomaterials. The fabrication strategies of CNF-based functional nanomaterials by adding metallic nanoparticles (NPs), metal oxide NPs, alloy, silica, polymers, and others into CNFs are introduced and discussed. In addition, the sensor applications of CNF-based nanomaterials for detecting gas, strain, pressure, small molecule, and biomacromolecules are demonstrated in detail. This work will be beneficial for the readers to understand the strategies for fabricating various CNF-based nanomaterials, and explore new applications in energy, catalysis, and environmental science.

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Carbon nanofiber-based multiplexed immunosensor for the detection of survival motor neuron 1, cystic fibrosis transmembrane conductance regulator and Duchenne Muscular Dystrophy proteins

Cited by 19 publications

References 47 publications

Combination of Carbon Nanofiber-Based Electrochemical Biosensor and Cotton Fiber: A Device for the Detection of the Middle-East Respiratory Syndrome Coronavirus

Combination of Carbon Nanofiber-Based Electrochemical Biosensor and Cotton Fiber: A Device for the Detection of the Middle-East Respiratory Syndrome Coronavirus

Multiplexed Immunosensors and Immunoarrays

Carbon Nanofiber-Based Functional Nanomaterials for Sensor Applications

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