Quantitative Analysis of Spectinomycin and Lincomycin in Poultry Eggs by Accelerated Solvent Extraction Coupled with Gas Chromatography Tandem Mass Spectrometry

Abstract

A method based on accelerated solvent extraction (ASE) coupled with gas chromatography tandem mass spectrometry (GC-MS/MS) was developed for the quantitative analysis of spectinomycin and lincomycin in poultry egg (whole egg, albumen and yolk) samples. In this work, the samples were extracted and purified using an ASE350 instrument and solid-phase extraction (SPE) cartridges, and the parameters of the ASE method were experimentally optimized. The appropriate SPE cartridges were selected, and the conditions for the derivatization reaction were optimized. After derivatization, the poultry egg (whole egg, albumen and yolk) samples were analyzed by GC-MS/MS. This study used blank poultry egg (whole egg, albumen and yolk) samples to evaluate the specificity, sensitivity, linearity, recovery and precision of the method. The linearity (5.6–2000 μg/kg for spectinomycin and 5.9–200 μg/kg for lincomycin), correlation coefficient (≥0.9991), recovery (80.0%–95.7%), precision (relative standard deviations, 1.0%–3.4%), limit of detection (2.3–4.3 μg/kg) and limit of quantification (5.6–9.5 μg/kg) of the method met the requirements for EU parameter verification. Compared with traditional liquid–liquid extraction methods, the proposed method is fast and consumes less reagents, and 24 samples can be processed at a time. Finally, the feasibility of the method was evaluated by testing real samples, and spectinomycin and lincomycin residues in poultry eggs were successfully detected.

Veterinary Drug Residues in Animal-Derived Foods: Sample Preparation and Analytical Methods

“…CNWBOND LC-C18 SPE cartridges can effectively extract eight COCs from eggs, and EVOLUTE ABN SPE cartridges, OASIS HLB SPE cartridges and Hybrid SPE cartridges can simultaneously extract multiple residues of veterinary drugs from animal-derived foods. Because of the good extraction efficiency of OASIS HLB SPE cartridges, they are widely used for veterinary drug residues in animal-derived foods [ 12 , 63 , 64 , 65 , 66 , 67 ]. The SPE method is widely used in the extraction of veterinary drug residues from animal-derived foods.…”

“…Wang et al [ 12 ] have developed a fast and sensitive ASE method coupled with gas chromatography-tandem mass spectrometry (GC-MS/MS) for the detection of spectinomycin and lincomycin in poultry eggs. This study used an ASE350 instrument and an Oasis PRiME HLB SPE cartridge to extract and purify egg poultry samples.…”

“…Compared with NPDs and ECDs, MS or MS/MS has good recovery, precision, and reproducibility and can confirm false positives. Generally, derivatization reactions are required for the detection of veterinary drugs by GC [ 12 , 119 ]. GC usually requires the selection of specific capillary columns to separate the veterinary drugs in the sample, while optimization of the mobile phase, as in the LC method, is not required.…”

“…Therefore, many detection techniques have been developed to detect veterinary drug residues in animal-derived foods. Currently, the classic analysis methods commonly used for veterinary drugs include enzyme-linked immunosorbent assay (ELISA) [ 9 ], capillary electrophoresis (CE) [ 10 ], liquid chromatography (LC) [ 11 ] and gas chromatography (GC) [ 12 ]. Generally, these methods have high sensitivity and selectivity in the detection of animal-derived foods.…”

Application of Analytical Chemistry to Foods and Food Technology

“…In a paper by Wang et al (2020), quantitative analysis of spectinomycin and lincomycin in poultry egg samples was performed by accelerated solvent extraction (ASE) coupled with gas chromatography tandem mass spectrometry (GC-MS/MS). The results show that the proposed method is faster, requires fewer reagents and more samples can be processed at a time, compared to conventional extraction methods [ 7 ].…”