“…Since CSF contains more than 7 n-mole/ml. choline phospholipids (Sastry and Stancer, 1968), this acetylation procedure may cause an in vitro increase of choline to be acetylated. In fact, Bowers (1967) reported different choline concentrations when acetylation was carried out with acetic anhydride before and after removal of proteins and phospholipids from the CSF.…”