Quantitation of Phytoestrogens in Legumes by HPLC

Franke, Adrian A.; Custer, Laurie J.; Cerna, Carmencita M.; Narala, Kavitha

doi:10.1021/jf00045a015

Cited by 275 publications

(170 citation statements)

References 43 publications

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Section: Isoflavones: Plant Molecules With Oestrogen-like Propertiesmentioning

confidence: 99%

“…Soy and soy products take on added nutritional importance because they are the primary foods, if not the only foods, that supply isoflavones in the diets of human populations. Several reports include the content of phyto-oestrogens in diverse beans [5][6][7][8] .With the increasing availability of isoflavoneenriched supplements and new soy-containing food items in the marketplace, the total intake of isoflavones in the US population, and possibly in other western nations, will most likely rise substantially over the next few decades. Females are the more likely consumers of these soy-containing foods because of their belief in the potential prevention of osteoporosis, cardiovascular disease, hormone-dependent cancers and the reduction of symptoms associated with the menopause.…”

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confidence: 99%

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Health potential of soy isoflavones for menopausal women

Anderson¹,

Anthony

Cline

et al. 1999

Public Health Nutr.

142

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Objective: To review the current literature on the effects of soy isoflavones, one class of phyto-oestrogens, on cardiovascular diseases, osteoporosis, cancer and climacteric symptoms. Design: Many study designs were employed in the reports reviewed here, including prospective human trials, observational human studies, animal experiments and in vitro cell studies that explored the protective or preventive effects of soy isoflavones (genistein, daidzein and glycitein alone or mixed). Setting: Diverse settings were employed, depending on study design. Subjects: Human subjects, mostly menopausal or postmenopausal, were included, as were animal models and specific cell types. Results: The findings were: (i) isoflavones plus soy protein together were needed to obtain the highly significant beneficial results on blood lipids and arterial dimensions; (ii) isoflavone treatments alone at high doses (relative to above) consistently improved bone parameters in rodent ovariectomized models, but not in humans or primates; (iii) isoflavones were not consistent in exerting positive effects regarding the prevention or treatment of cancers of the mammary glands, uterus and colon; and (iv) the effects of isoflavones on climacteric symptoms were not clear-cut. Conclusions:The promise of soy isoflavones reducing chronic disease risk seems to be non-uniform, with the most conclusive benefits occurring in the prevention of cardiovascular diseases, but other organ systems, such as skeletal and reproductive tissues, may also benefit from the consumption of soy and soy-derived products. Phyto-oestrogenic molecules have received a great deal of attention over the last few years because of their potentially preventive roles against a few of today's most prevalent chronic diseases, namely, cardiovascular disease, osteoporosis and hormone-related cancers 1,2 . The particular plant molecules of interest are isoflavones, which are found in abundance in soybeans and their derivative foods, such as tofu, miso and others. Of the several isoflavones that are made by soybeans, genistein, in particular, has been experimentally shown to be the most efficacious in human subjects and animal models. For the last several years, a concerted effort has been mounted to identify alternatives to traditional hormone replacement therapy (HRT). Pharmaceutical companies have been developing selective oestrogen receptor modulators (SERMs), which ideally will provide the beneficial effects of oestrogen replacement for bones, cardiovascular system and cognitive function, without adverse effects on breast and endometrial cancer. As a result, SERMs should not require co-administration with a progestin, which can eliminate the side effects that some women experience with that hormone. The first molecules of this new class of drugs to become available are tamoxifen and raloxifene, which exhibit many, but not all, of the qualities of an ideal HRT. Many consumers would also prefer to avoid synthetic molecules in favour of natural products. Soy phytooestrogens, na...

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Section: Isoflavones: Plant Molecules With Oestrogen-like Propertiesmentioning

confidence: 99%

mentioning

confidence: 99%

Health potential of soy isoflavones for menopausal women

Anderson¹,

Anthony

Cline

et al. 1999

Public Health Nutr.

142

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“…Previously, reversed-phase high-performance liquid chromatography (HPLC) with UV, fluorescence, or electrochemical detection have been reported for detection of isoflavones in kudzu root [7,8], soybean [9,10], and other plants [11,12]. These methods are limited to the detection of a limited number of known compounds, however, and cannot be used for characterization of unknown isoflavones in a crude mixture.…”

Section: Introductionmentioning

confidence: 99%

High-performance liquid chromatography–tandem mass spectrometry for identification of isoflavones and description of the biotransformation of kudzu root

Zhang

et al. 2005

Anal Bioanal Chem

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High-performance liquid chromatography-tandem mass spectrometry has been used to identify isoflavone aglycones and glycosides in kudzu root. Fourteen isoflavones were detected. Among these, six were identified by comparison with authentic standards. Tentative identifications of the other isoflavones are based on UV spectra, mass spectra of protonated and deprotonated molecules, and MS-MS data. Several are reported for the first time in kudzu root. The bioactivity and bioavailability of isoflavone aglycones are usually greater than those of their glycosides. To improve the bioavailability of kudzu root isoflavones, crude β-glycosidases prepared from microbes were used to hydrolyze the isoflavone glycosides. Several MS modes are combined not only to identify the isoflavones in kudzu root, but also to describe the biotransformation of kudzu root isoflavone glycosides. It is also proved that crude β-glycosidases have high selectivity toward the O-glycosides of isoflavones.

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“…Most abundant ones are daidzein (7,4'-dihydroxyisoflavone), genistein (4' ,5,7-trihydroxyisoflavone), formononetin (7-hydroxy-4'-methoxyisoflavone), biochanin A (5,7-dihydroxy-4'-metoxyizoflavon), and coumestrol. In plants they are mostly present in the form of 6''-O-acetyl or malonyl 6''-O-malonyl derivates (Franke et al 1994;Morton et al 1999).…”

mentioning

confidence: 99%

Detection of plant raw materials in meat products by HPLC

Vaňha¹,

Hinkova²,

Sluková³

et al. 2009

Czech J. Food Sci.

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Vaňha J., Hinková A., Sluková M., Kvasnička F. (2009): Detection of plant raw materials in meat products by HPLC. Czech J. Food Sci., 27: 234-239.The Czech legislation (Decrees No. 326/2001, 202/2003 and 651/2004 of the law No. 110/1997 as amended) regulates the requirements for the selected meat products with regard to the contents of individual ingredients. However, the methods of the determination of compliance with these regulations are not closely specified. The study presented here deals with the development and verification of analytical methods suitable for the detection of the material of plant origin. Due to the high variability in the contents in meat products of these ingredients, various markers were observed (isoflavones, phytic acid, galactooligosaccharides). For the purpose of detection, substances commonly used in food processing industries were taken into account such as soy flour, wheat flour, soy isolate, HAM 60 preparation. The values gained by measuring the given markers were subsequently converted to reflect the amount of the plant based substance added. Out of 18 products commonly available in shops, only 7 filfilled the legal criteria.

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Quantitation of Phytoestrogens in Legumes by HPLC

Cited by 275 publications

References 43 publications

Health potential of soy isoflavones for menopausal women

Health potential of soy isoflavones for menopausal women

High-performance liquid chromatography–tandem mass spectrometry for identification of isoflavones and description of the biotransformation of kudzu root

Detection of plant raw materials in meat products by HPLC

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