Quantifying Skeletal Muscle Properties in Cadaveric Test Specimens: Effects of Mechanical Loading, Postmortem Time, and Freezer Storage

Ee, Chris A. Van; Chasse, A. L.; Myers, Barry S.

doi:10.1115/1.429621

Cited by 128 publications

(101 citation statements)

References 28 publications

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“…More importantly, Morrow et al, 2010 froze their samples within an hour of the animal sacrifice, but no effort was made to discuss the thawing process they used. Although it has been found that a freeze thaw cycle does not affect the post rigor response of muscle tissue (Van Ee et al, 2000), the thawing process remains an important part of their experiments as it is likely to have allowed rigor mortis to set in and make the tissue much stiffer (the rigor mortis characterisation investigation performed on freshly harvested porcine tissue (Van Loocke, et al,. 2006, showed that the testing window is two hours after death before rigor mortis starts influencing the passive response of porcine skeletal muscles).…”

Section: Interpretation Of Resultsmentioning

confidence: 99%

The anisotropic mechanical behaviour of passive skeletal muscle tissue subjected to large tensile strain

Takaza

Moerman

Gindre

et al. 2013

Journal of the Mechanical Behavior of Biomedical Materials

130

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The anisotropic mechanical behaviour of passive skeletal muscle tissue subjected to large tensile strain, Journal of the Mechanical Behavior of Biomedical Materials, http://dx.doi.org/10. 1016/j.jmbbm.2012.09.001 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractThe passive mechanical properties of muscle tissue are important for many biomechanics applications. However, significant gaps remain in our understanding of the three-dimensional tensile response of passive skeletal muscle tissue to applied loading. In particular, the nature of the anisotropy remains unclear and the response to loading at intermediate fibre directions and the Poisson's ratios in tension have not been reported. Accordingly, tensile tests were performed along and perpendicular to the muscle fibre direction as well as at 30, 45 and 60 degrees to the muscle fibre direction in samples of Longissimus dorsi muscle taken from freshly slaughtered pigs. Strain was measured using an optical non-contact method. The results show the transverse or cross fibre (TT') direction is broadly linear and is the stiffest (77kPa stress at a stretch of 1.1), but that failure occurs at low stretches (approximately λ = 1.15). In contrast the longitudinal or fibre direction (L) is nonlinear and much less stiff (10kPa stress at a stretch of 1.1) but failure occurs at higher stretches (approximately λ = 1.65). An almost sinusoidal variation in stress response was observed at intermediate angles. The following Poisson's ratios were measured: Ѵ LT = Ѵ LT' = 0.47, Ѵ TT' = 0.28 and Ѵ TL = 0.74. These observations have not been previously reported and they contribute significantly to our understanding of the three dimensional deformation response of skeletal muscle tissue.

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Section: Interpretation Of Resultsmentioning

confidence: 99%

The anisotropic mechanical behaviour of passive skeletal muscle tissue subjected to large tensile strain

Takaza

Moerman

Gindre

et al. 2013

Journal of the Mechanical Behavior of Biomedical Materials

130

View full text Add to dashboard Cite

show abstract

“…There is also a paucity of good quality research in human tissue properties due to difficulties in obtaining post-mortem human subjects and the degradation in properties associated with the conditions the body is kept post-mortem prior to testing (Van Ee et al, 2000) and storage conditions (Menz, 1971;Lee & Pelker, 1985;Clavert et al, 2001). Consequently, very few studies presented are of human tissues (Chawla et al, 2009;Balaraman et al, 2012).…”

Section: Structure and Composition Of Organic Muscle Tissuementioning

confidence: 99%

Development of novel synthetic muscle tissues for sports impact surrogates

Payne

Mitchell

Bibb

et al. 2015

Journal of the Mechanical Behavior of Biomedical Materials

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Impact injuries are commonplace in sport and often lead to performance detriment and debilitation. Personal Protective Equipment (PPE) is prescribed as a mandatory requirement in most sports where these impacts are likely to occur, though the methods of governance and evaluation criteria often do not accurately represent sports specific injury scenarios. One of the key shortcomings of such safety test standards is the human surrogate to which the PPE is affixed; this typically embodies unrepresentative geometries, masses, stiffness and levels of constraint when compared to humans.A key aspect of any human surrogate element is the simulant material used. Most previous sports specific surrogates tend to use off-the-shelf silicone blends to represent all the soft tissue structures within the human limb segment or organ; this approach potentially neglects important human response phenomena caused by the different tissue structures.This study presents an investigation into the use of bespoke additive cure Polydimethysiloxane (PDMS) silicone blends to match the reported mechanical properties of human relaxed and contracted skeletal muscle tissues. The silicone simulants have been tested in uniaxial compression through a range of strain rates and fit with a range of constitutive hyperelastic models (Mooney Rivlin, Ogden and Neo Hookean) and a viscoelastic Prony series.

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“…However, when performed on each experimental group, the multi linear regressions showed that up to 60% or 78% of E can be explained by the MRI parameters in the rigor mortis (Equation (2)) and post-rigor mortis (Equation (3)) groups respectively. The highest variance inflation factor (VIF) was attributed to ADC in Equation (1) and to MTR in Equation (2), suggesting that these parameters were likely candidates for elimination in the equations. However, in Equation (3), all the parameters presented equivalent VIFs.…”

Section: Multi Linear Regressionsmentioning

confidence: 99%

“…Even if the material properties vary greatly during rigor mortis [2], there was less than one hour between the MRI acquisition and the mechanical tests, leading to acceptable changes in the rigor mortis group between the two tests.…”

Section: Samples Preparationmentioning

confidence: 99%

“…We chose these two groups to obtain a large range of muscles rigidities, higher than the one obtained between the different muscles [2]. Even if the material properties vary greatly during rigor mortis [2], there was less than one hour between the MRI acquisition and the mechanical tests, leading to acceptable changes in the rigor mortis group between the two tests.…”

Section: Samples Preparationmentioning

confidence: 99%

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Assessment of Mechanical Properties of Muscles from Multi-Parametric Magnetic Resonance Imaging

Grenier¹,

Périé

Gilbert³

et al. 2014

JBiSE

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We hypothesized that a relationship existed between the mechanical properties and the magnetic resonance imaging (MRI) parameters of muscles, as already demonstrated in cartilaginous tissues. The aim was to develop an indirect evaluation tool of the mechanical properties of degenerated muscles. Leg and arm muscles of adult rabbits were dissected, and tested 12 hours post mortem, in a state of rigor mortis, or 72 hours post mortem, in a state of post-rigor mortis. The tests consisted of a multi-parametric MRI acquisition followed by a uniaxial tensile test until failure. The statistical analysis consisted of multiple linear regressions and principal component analysis. Significant differences existed between the rigor mortis and post-rigor mortis groups for E but not for the MRI parameters. 78%, 60% or 33% of the Young's modulus could be explained by the MRI parameters in the post-rigor mortis group, rigor mortis group or both groups respectively. These relationships were confirmed by the principal component analysis. The proposed multi-parametric MRI protocol associated to principal component analysis is a promising tool for the indirect evaluation of muscle mechanical properties and should be useful to find biomarkers and predictive factors of the evolution of the pathologies.

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Quantifying Skeletal Muscle Properties in Cadaveric Test Specimens: Effects of Mechanical Loading, Postmortem Time, and Freezer Storage

Cited by 128 publications

References 28 publications

The anisotropic mechanical behaviour of passive skeletal muscle tissue subjected to large tensile strain

The anisotropic mechanical behaviour of passive skeletal muscle tissue subjected to large tensile strain

Development of novel synthetic muscle tissues for sports impact surrogates

Assessment of Mechanical Properties of Muscles from Multi-Parametric Magnetic Resonance Imaging

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