Assessment of Mechanical Properties of Muscles from Multi-Parametric Magnetic Resonance Imaging

Grenier, Renaud; Périé, Delphine; Gilbert, Guillaume; Beaudoin, G.; Curnier, Daniel

doi:10.4236/jbise.2014.78060

Cited by 6 publications

(3 citation statements)

References 41 publications

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“…Furthermore, T1 and T2 have been shown to correlate with mechanical properties of native and engineered tissues. [ 26,37,38 ] T1, T2, and ADC values were acquired from the samples illustrated in Figure 7 d-f and there was no signifi cant change in any of these parameters over the culture period suggesting that the graft was stable. All of the values for the engineered tissue samples where higher than for native articular cartilage (Table S1, Supporting Information).…”

Section: Magnetic Resonance Imagingmentioning

confidence: 99%

Bioprinting Complex Cartilaginous Structures with Clinically Compliant Biomaterials

Kesti

Eberhardt²,

Pagliccia

et al. 2015

Adv Funct Materials

195

149

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wileyonlinelibrary.comwith rapid production of patient-specifi c grafts, allowing precise control over internal and external architecture and customized mechanical properties. These techniques can be used for printing biological materials together with living cells, hence the term "bioprinting." 3D bioprinting offers researchers a unique way of depositing cell-laden biocompatible materials, the so-called bioinks, in high-resolution structures with a line thickness on the order of hundreds of microns. Due to the promise of such a technology, several commercial bioprinters have entered the market and bioinks are the subject of intense investigation. [1][2][3] Bioink formulation is often considered one of the most critical aspects of high-resolution cellular bioprinting.Cellular printing requires a bioink with two key properties, namely printability and cytocompatible crosslinking. The identifi cation of printable polymeric systems is mainly done through rheological evaluation of a material's shear thinning behavior and shear recovery. Shear thinning correlates directly with a bioink's ability to be extruded at low pressure (<3 bar), something which ensures high postprinting cell viability. [ 4 ] Shear recovery, on the other hand, relates to the ink's resistance to fl ow after printing, which ensures high fi delity of the printed structure. The presence of cells, however, greatly restricts the crosslinking options as physiologic temperature and pH need to be maintained and harsh chemicals avoided. Hydrogel bioinks can be crosslinked via covalent or physical interactions or a combination thereof. Ultraviolet light initiated crosslinking of (meth)acrylated polymers has been used most often in bioinks, but the presence of potentially toxic monomers and photoinitiators may complicate clinical translation. [5][6][7][8] Physically crosslinked gelation based on temperature, hydrophobic/hydrophilic or ionic interactions has been utilized for precrosslinking of several bioink materials including poly( N -isopropylacrylamide) conjugated hyaluronan (HA-pNIPAAm), [ 9 ] gelatin, [ 10,11 ] alginate, [ 12 ] and gellan. [ 13 ] Precrosslinking before printing or directly during deposition to stabilize the printed lines is generally followed by a fi nal crosslinking which further increases the mechanical properties and stabilizes the whole structure.For cartilage engineering applications, natural polymers from animal or plant sources including alginate, collagen, gelatin, Bioprinting is an emerging technology for the fabrication of patient-specifi c, anatomically complex tissues and organs. A novel bioink for printing cartilage grafts is developed based on two unmodifi ed FDA-compliant polysaccharides, gellan and alginate, combined with the clinical product BioCartilage (cartilage extracellular matrix particles). Cell-friendly physical gelation of the bioink occurs in the presence of cations, which are delivered by co-extrusion of a cation-loaded transient support polymer to stabilize overhanging structures. Rheological properties of...

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Section: Magnetic Resonance Imagingmentioning

confidence: 99%

Bioprinting Complex Cartilaginous Structures with Clinically Compliant Biomaterials

Kesti

Eberhardt²,

Pagliccia

et al. 2015

Adv Funct Materials

195

149

View full text Add to dashboard Cite

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“…One potential influencing factor is that the hydrogels were much stiffer than values reported for the native human NP of ∼5–25 kPa ( Cloyd et al, 2007 ; Borrelli and Buckley 2020 ) and bovine NP of ∼6–19 kPa ( Recuerda et al, 2011 ; Grenier et al, 2014 ). Notably, Gilchrist et al (2011) demonstrated the importance of both ECM ligands and substrate stiffness on NP phenotype, with a more native NP morphology, enhanced cell-cell interactions, and augmented proteoglycan synthesis when porcine NP cells were cultured on soft (<720 Pa) ECM substrates that contained laminin.…”

Section: Discussionmentioning

confidence: 95%

Development of 2-D and 3-D culture platforms derived from decellularized nucleus pulposus

Quijano

Sharma²,

Martin³

et al. 2022

Front. Bioeng. Biotechnol.

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Bioscaffolds derived from the extracellular matrix (ECM) have shown the capacity to promote regeneration by providing tissue-specific biological instructive cues that can enhance cell survival and direct lineage-specific differentiation. This study focused on the development and characterization of two-dimensional (2-D) and three-dimensional (3-D) cell culture platforms incorporating decellularized nucleus pulposus (DNP). First, a detergent-free protocol was developed for decellularizing bovine nucleus pulposus (NP) tissues that was effective at removing cellular content while preserving key ECM constituents including collagens, glycosaminoglycans, and the cell-adhesive glycoproteins laminin and fibronectin. Next, novel 2-D coatings were generated using the DNP or commercially-sourced bovine collagen type I (COL) as a non-tissue-specific control. In addition, cryo-milled DNP or COL particles were incorporated within methacrylated chondroitin sulphate (MCS) hydrogels as a 3-D cell culture platform for exploring the effects of ECM particle composition. Culture studies showed that the 2-D coatings derived from the DNP could support cell attachment and growth, but did not maintain or rescue the phenotype of primary bovine NP cells, which de-differentiated when serially passaged in monolayer culture. Similarly, while bovine NP cells remained highly viable following encapsulation and 14 days of culture within the hydrogel composites, the incorporation of DNP particles within the MCS hydrogels was insufficient to maintain or rescue changes in NP phenotype associated with extended in vitro culture based on gene expression patterns. Overall, DNP produced with our new decellularization protocol was successfully applied to generate both 2-D and 3-D bioscaffolds; however, further studies are required to assess if these platforms can be combined with additional components of the endogenous NP microenvironment to stimulate regeneration or lineage-specific cell differentiation.

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“…Equivalent experiments were done on intervertebral discs and showed that 45 to 80% of the Young’s modulus, the aggregate modulus, the radial permeability and the axial permeability can be explained mostly by MT and diffusion sequences [60]. On the skeletal muscle, up to 78% of the Young’s modulus can be explained by relaxation times, magnetization transfer and diffusion coefficients suggesting a linear relationship [61]. However, both studies showed changes in the relationships when the tissue is degenerated with a significant modification of the mechanical properties, suggesting that before the use of this technique to quantify the mechanical properties in vivo on patients suffering from various diseases, the relationships have to be defined for each degeneration state of the tissue that mimics the pathology.…”

Section: Discussionmentioning

confidence: 99%

Multi-parametric MRI as an indirect evaluation tool of the mechanical properties of in-vitrocardiac tissues

Périé

Dahdah

Foudis

et al. 2013

BMC Cardiovasc Disord

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BackgroundEarly detection of heart failure is essential to effectively reduce related mortality. The quantification of the mechanical properties of the myocardium, a primordial indicator of the viability of the cardiac tissue, is a key element in patient’s care. Despite an incremental utilization of multi-parametric magnetic resonance imaging (MRI) for cardiac tissue characteristics and function, the link between multi-parametric MRI and the mechanical properties of the heart has not been established. We sought to determine the parametric relationship between the myocardial mechanical properties and the MR parameters. The specific aim was to develop a reproducible evaluative quantitative tool of the mechanical properties of cardiac tissue using multi-parametric MRI associated to principal component analysis.MethodsSamples from porcine hearts were submitted to a multi-parametric MRI acquisition followed by a uniaxial tensile test. Multi linear regressions were performed between dependent (Young’s modulus E) and independent (relaxation times T1, T2 and T2*, magnetization transfer ratio MTR, apparent diffusion coefficient ADC and fractional anisotropy FA) variables. A principal component analysis was used to convert the set of possibly correlated variables into a set of linearly uncorrelated variables.ResultsValues of 46.1±12.7 MPa for E, 729±21 ms for T1, 61±6 ms for T2, 26±7 for T2*, 35±5% for MTRx100, 33.8±4.7 for FAx10-2, and 5.85±0.21 mm2/s for ADCx10-4 were measured. Multi linear regressions showed that only 45% of E can be explained by the MRI parameters. The principal component analysis reduced our seven variables to two principal components with a cumulative variability of 63%, which increased to 80% when considering the third principal component.ConclusionsThe proposed multi-parametric MRI protocol associated to principal component analysis is a promising tool for the evaluation of mechanical properties within the left ventricle in the in vitro porcine model. Our in vitro experiments will now allow us focused in vivo testing on healthy and infracted hearts in order to determine useful quantitative MR-based biomarkers.

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Assessment of Mechanical Properties of Muscles from Multi-Parametric Magnetic Resonance Imaging

Cited by 6 publications

References 41 publications

Bioprinting Complex Cartilaginous Structures with Clinically Compliant Biomaterials

Bioprinting Complex Cartilaginous Structures with Clinically Compliant Biomaterials

Development of 2-D and 3-D culture platforms derived from decellularized nucleus pulposus

Multi-parametric MRI as an indirect evaluation tool of the mechanical properties of in-vitrocardiac tissues

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