Quantification of zidovudine and one of its metabolites in plasma and urine by solid-phase extraction and high-performance liquid chromatography

Nucleoside reverse transcriptase inhibitors and their phosphorylated metabolites in human immunodeficiency virus-infected human matrices

“…At pH > 6, the recovery of AMT almost decreased to 0. Schrive and Plasse suggested an on-line SPE method to extract AZT and G-AZT from plasma and urine with a Perisorb RP8 extraction column [55]. The on-line method provided rapid sample pretreatment because it circumvented the evaporation under nitrogen flow.…”

Reversed phase HPLC determination of zidovudine in rat plasma and its pharmacokinetics after a single intranasal dose administration

“…The HPLC methods described in the literature for determination of AZT levels in animal and human plasma (Molema et al, 1992;Schrive and Plasse, 1994;Aymard et al, 2000;Tan and Boudinot, 2000;Clark et al, 2001;Marchei et al, 2002;Ramachandran et al, 2006;Tarinas et al, 2007;Lewis et al, 2007;Lefebvre et al, 2007) present high analysis costs of because they use sample treatment processes based on solid phase extraction or several steps of liquid-liquid extraction. These assay methods are relatively time-consuming or use expensive instrumentation not readily accessible to many groups.…”

“…These methods involve ultraviolet assay (Baig et al, 2001;Erk, 2004), liquid chromatography/tandem mass spectrometry Pereira et al, 2000;Alnouti et al, 2005;Mudigonda et al, 2008), micellar capillary electrophoresis and high-performance liquid chromatography (HPLC) with UV detection (Molema et al, 1992;Schrive and Plasse, 1994;Aymard et al, 2000;Tan and Boudinot, 2000;Clark et al, 2001;Marchei et al, 2002;Ramachandran et al, 2006;Tarinas et al, 2007;Lewis et al, 2007;Lefebvre et al, 2007). Most of the methods using HPLC with UV detection methods described until now are relatively timeconsuming or they use expensive instrumentation not readily accessible to many groups.…”

HPLC in biopharmaceutical investigations of drugs representing pyrimidine derivatives (A review)

“…The latter cartridge ensured the best reproducibility of results and provided better purification of samples from interfering substances. The stage of deproteination was performed using organic solvents [29 -34], trichloroacetic acid [35], and perchloric acid [21], or by means of ultrafiltration [36,37]. Foreign macromolecules were successfully removed on a Spheron Thiol column (100´4 mm, 40 -63 mm) containing silver(I) ions [38,39].…”