The HPLC capacity factors of 27 flavonoids were determined using the following chromatographic conditions: Zorbax SB 250´4.6 mm analytical columns containing C-18, C-8, and CN sorbents with particle size 5 mm, mobile phase consisting of 0.01 M potassium dihydrogen phosphate pH 3.0 and acetonitrile at a ratio of 80:20 (v/v), column temperature 30°C; absorption spectra were recorded over the range 190 -400 nm. This chromatography system can be used for preliminary identification of flavonoids in plant materials.
Pyrimidine derivatives are widely used for the treatment of viral diseases and cancer. The analysis of pyrimidine derivatives is typically performed using various chromatographic techniques, in particular, reversed-phase high performance liquid chromatography (HPLC). The separation is typically carried out with (7 -30)-cm-long C 8 and C 18 silica gel columns, mainly at room temperature, and a 1 -1.5 ml/min eluent flow rate. The column is eluted in an isocratic or gradient system, and a variety of mobile phases have been proposed. The detection is based on optical absorption or fluorescence measurements, or makes use of mass spectrometry. Various methods of extraction of pyrimidine derivatives from biological samples are discussed, and the corresponding detection limits are presented.
An HPLC-based method for determining cytarabine (1-b-D-arabinofuranosylcytosine, Ara-C) and uracil arabinoside (Ara-U, a product of the hydrolytic deamination of Ara-C), as well other related compounds (cytidine, cyclocytidine, uridine), in aqueous solutions has been developed. The proposed method is characterized by good reproducibility and is capable of quantitatively determining all compounds simultaneously present in solution.
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