Quantification of vitamin B6 vitamers in human cerebrospinal fluid by ultra performance liquid chromatography–tandem mass spectrometry

Ham, Maria van der; Albersen, Monique; Koning, Tom J. de; Visser, Gepke; Middendorp, A.; Bosma, Marjolein; Verhoeven‐Duif, Nanda M.; Velden, Monique G.M. de Sain–van der

doi:10.1016/j.aca.2011.11.018

Cited by 64 publications

(68 citation statements)

References 29 publications

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“…; heater gas of 60 p.s.i., and interface heating temperature of 200°C. MS 2 scans were collected by the third quadrupole operating in resolving quadrupole mode with a scan time of 5 s. Collisioninduced dissociation generated a single fragment ion for each analyte, consistent with previous studies (57) and in agreement with the published values (57). Hence, only one transition was used to quantify each B 6 vitamer (m/z 1693134 for PM; m/z 1723137 for d 3 -PM; m/z 1683150 for PL; m/z 1703134 for pyridoxine, and m/z 2483150 for PLP).…”

Section: Methodsmentioning

confidence: 77%

“…An Ultimate 3000 liquid chromatography system (Dionex) equipped with a Zorbax SB-AQ column (2.1 ϫ 100 mm, 3.5 m, Agilent) with matching guard column (2.1 ϫ 12.5 mm, 5 m) was used for separation. Column temperature was maintained at 25°C, and the injection volume was 20 l. The liquid chromatography gradient was described previously (57). Briefly, initial chromatographic conditions were 99% solvent A (650 mM acetic acid containing 0.01% heptafluorobutyric acid) and 1% solvent B (acetonitrile).…”

Section: Methodsmentioning

confidence: 99%

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Pretreatment with Pyridoxamine Mitigates Isolevuglandin-associated Retinal Effects in Mice Exposed to Bright Light

Charvet

Saadane

Wang

et al. 2013

Journal of Biological Chemistry

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Background: Current antioxidant therapies do not directly target lipid peroxidation products. Results: Pyridoxamine treatment scavenges lipid peroxides in mouse retina, as exemplified by isolevuglandins, and improves retinal mitochondrial morphology after animal exposure to bright light. Conclusion: Pyridoxamine reduces deleterious effects of lipid peroxidation in the retina. Significance: Pyridoxamine supplementation should be considered for inclusion in antioxidant vitamin formulations.

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Section: Methodsmentioning

confidence: 77%

Section: Methodsmentioning

confidence: 99%

Pretreatment with Pyridoxamine Mitigates Isolevuglandin-associated Retinal Effects in Mice Exposed to Bright Light

Charvet

Saadane

Wang

et al. 2013

Journal of Biological Chemistry

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“…In human cerebrospinal fluid pyridoxal is the dominant B-6 vitamer van der Ham et al 2012;Albersen et al 2014). Median values for pyridoxal and pyridoxal phosphate in the cerebrospinal fluid of infants declined over 50 % between a postmenstrual age of 30-37 and ≥42 weeks .…”

Section: Absorption and Transport Of Vitamin B-6mentioning

confidence: 98%

Vitamin B-6 Metabolism and Interactions with TNAP

Coburn

2015

Subcellular Biochemistry

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“…Most separation and detection procedures are reversed-phase high-performance liquid chromatography (HPLC) techniques (Eitenmiller et al 2008) (Ciulu et al 2011;Jin et al 2012;Langer and Lodge 2014), and MS (Hälvin et al 2013;van der Ham et al 2012). Methods using electrochemical detection (ED) have also been proposed but applications to samples other than pharmaceuticals supplements and animal foods are few (Lebiedzińska et al 2007;Marszałł et al 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Pyridoxal-5′-phosphate (PLP), on the other hand, has been reported to be the most biologically active form of vitamin B 6 and functions as a co-factor crucial for numerous enzymatic reactions (di Salvo et al 2012;Eitenmiller et al, 2008;van der Ham et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Detection of B6 Vitamers in Grain Products: Experimental and Computational Studies

et al. 2017

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A reversed phase high-performance liquid chromatography method (HPLC) with coulometric electrochemical detection has been applied and validated for the simultaneous analysis of pyridoxamine (PM), pyridoxal (PL), and pyridoxine (PN) in cereal products. Isocratic separation was achieved using a C 18 column with a mobile phase consisting of methanol-phosphate buffer (10:90) and 0.018 M trimethylamine adjusted to pH 3.55 with 85% orthophosphoric acid. The limits of detection for PM, PL, and PN were 0.28, 0.36, and 0.43 ng mL −1 , respectively, with vitamins recoveries ranging from 90.4 to 98.1%. The applied method for the analysis of B 6 vitamins naturally present in grain products, offers a simple and fast sample preparation without derivatization. To understand vitamer separation further, methods of computational chemistry were employed. Specifically, density functional theory (DFT) was used to determine electrostatic potentials, as well as the charges of each vitamer. A number of correlations were established between these properties and elution order.

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Quantification of vitamin B6 vitamers in human cerebrospinal fluid by ultra performance liquid chromatography–tandem mass spectrometry

Cited by 64 publications

References 29 publications

Pretreatment with Pyridoxamine Mitigates Isolevuglandin-associated Retinal Effects in Mice Exposed to Bright Light

Pretreatment with Pyridoxamine Mitigates Isolevuglandin-associated Retinal Effects in Mice Exposed to Bright Light

Vitamin B-6 Metabolism and Interactions with TNAP

Detection of B6 Vitamers in Grain Products: Experimental and Computational Studies

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