Detection of B6 Vitamers in Grain Products: Experimental and Computational Studies

Abstract

A reversed phase high-performance liquid chromatography method (HPLC) with coulometric electrochemical detection has been applied and validated for the simultaneous analysis of pyridoxamine (PM), pyridoxal (PL), and pyridoxine (PN) in cereal products. Isocratic separation was achieved using a C 18 column with a mobile phase consisting of methanol-phosphate buffer (10:90) and 0.018 M trimethylamine adjusted to pH 3.55 with 85% orthophosphoric acid. The limits of detection for PM, PL, and PN were 0.28, 0.36, and 0.43 ng mL −1 , respectively, with vitamins recoveries ranging from 90.4 to 98.1%. The applied method for the analysis of B 6 vitamins naturally present in grain products, offers a simple and fast sample preparation without derivatization. To understand vitamer separation further, methods of computational chemistry were employed. Specifically, density functional theory (DFT) was used to determine electrostatic potentials, as well as the charges of each vitamer. A number of correlations were established between these properties and elution order.

4′-O-methylpyridoxine: Preparation from Ginkgo biloba Seeds and Cytotoxicity in GES-1 Cells

“…Plant-based food is an important source for human to obtain VB 6 , as only plants and microorganisms can synthesize VB6 [39]. According to previous studies, the content of VB 6 in Ginkgo biloba seeds is 400.44-586.82 µg/g, which was higher than that of common foods (e.g., milk, wheat and potato) [40,41]. Some scholars speculated that MPN in Ginkgo seeds may act as an antagonist of VB 6 in the human body, for that MPN is similar to the PN form of VB 6 [42].…”

Recent Techniques in Nutrient Analysis for Food Composition Database

“…The commonly used extraction method for B complex vitamins analysis, including protein precipitation [ 108 , 113 , 117 , 123 ], acid hydrolysis and/or enzymatic treatment [ 109 , 114 , 118 ] and SPE [ 112 , 116 ]. Protein precipitation is a common method used for extraction of B complex vitamins from samples rich in protein like milk.…”

“…However, the disadvantage is time-consuming [ 124 ]. Recently, most widely used analytical method for B complex vitamin analysis are HPLC coupled with UV [ 110 , 116 ], FLD [ 104 , 106 , 108 , 123 ], DAD [ 115 ] and ED [ 109 ] detectors. However, the application of LC-MS/MS method for vitamins analysis has been increased due to its higher sensitivity and specificity especially in complex matrices allowing multivitamin analysis [ 117 , 118 ].…”

Increase in Nutrition and Biological Value of White Bread With Use of the Grain Mix «omega-6»

“…Properly selected recipes and specially prepared cereal mixtures are a source of nutrient deficiencies in bread. This bread belongs to an exceptional place in human nutrition, due to the content in it of components with nutritional and biological value [12][13][14][15][16].…”