Quantification of Specific Glycation Sites in Human Serum Albumin as Prospective Type 2 Diabetes Mellitus Biomarkers

Spiller, Sandro; Frolov, Andrej; Hoffmann, Ralf

doi:10.2174/0929866524666170202124120

Cited by 26 publications

(48 citation statements)

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“…Glycation sites previously identified in plasma proteins of patients with diabetes were quantified after tryptic digestion of plasma samples and consecutive enrichment by boronate affinity chromatography, using a recently optimized method with multiple reaction monitoring on an electrospray ionization mass spectrometer (ESI‐MS), coupled on‐line to reversed‐phase high‐performance liquid chromatography (RP‐HPLC) . The validated protocol quantifies 30 glycation sites in 9 plasma proteins with sensitivities in the low nanomolar range and high interday precisions, that is, coefficients of variation (CVs) below 20%.…”

Section: Reasearch Design and Methodsmentioning

confidence: 99%

“…cFFA levels were compared among four different age‐matched cohorts, that is, 20 individuals with prediabetes, 48 patients with newly diagnosed T2DM, 48 long‐term controlled T2DM patients and 48 non‐diabetic individuals. FFA levels were also evaluated in combination with other routine anthropometric and laboratory chemistry parameters and the glycation degrees of 30 plasma protein glycation sites recently reported to improve diagnostic accuracy in T2DM . Additionally, the ratios of total cholesterol to HDL‐c (total‐c/HDL‐c), TAG/HDL‐c, and LDL‐c/HDL‐c and TAG levels as known predictors of CVDs, diabetic kidney disease (DKD) and ischemic heart disease (IHD) were studied.…”

Section: Introductionmentioning

confidence: 99%

“…FFA levels were also evaluated in combination with other routine anthropometric and laboratory chemistry parameters and the glycation degrees of 30 plasma protein glycation sites recently reported to improve diagnostic accuracy in T2DM. 18,19 Additionally, the ratios of total cholesterol to HDL-c (total-c/HDL-c), TAG/HDL-c, and LDL-c/HDL-c and TAG levels as known predictors of CVDs, 20-23 diabetic kidney disease (DKD) 24 and ischemic heart disease (IHD) 25 were studied.…”

Section: Introductionmentioning

confidence: 99%

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Plasma levels of free fatty acids correlate with type 2 diabetes mellitus

Spiller

Blüher

Hoffmann

2018

Diabetes Obesity Metabolism

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FFA levels are elevated in newly diagnosed and long-term controlled T2DM patients, providing high diagnostic accuracy of 87% and 91%, respectively, which improved further when combined with the glycation degree of lysine-141 in haptoglobin. Additionally, FFA showed higher mean fold-changes than HbA1c or FPG in subjects developing T2DM, indicating higher sensitivity towards the progression of the disease.

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Section: Reasearch Design and Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Plasma levels of free fatty acids correlate with type 2 diabetes mellitus

Spiller

Blüher

Hoffmann

2018

Diabetes Obesity Metabolism

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“…In contrast to the experiments performed in a full MS mode, MRM represents a method of highly-sensitive targeted analysis [ 154 ]. Accordingly, it can be applied to a limited number of analytes, but each of them can be detected with a high precision, accuracy and sensitivity [ 76 ].…”

Section: Methods Of Amino Acid Analysis In Glycation Researchmentioning

confidence: 99%

Probing Protein Glycation by Chromatography and Mass Spectrometry: Analysis of Glycation Adducts

Soboleva

Vikhnina

Grishina

et al. 2017

IJMS

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Glycation is a non-enzymatic post-translational modification of proteins, formed by the reaction of reducing sugars and α-dicarbonyl products of their degradation with amino and guanidino groups of proteins. Resulted early glycation products are readily involved in further transformation, yielding a heterogeneous group of advanced glycation end products (AGEs). Their formation is associated with ageing, metabolic diseases, and thermal processing of foods. Therefore, individual glycation adducts are often considered as the markers of related pathologies and food quality. In this context, their quantification in biological and food matrices is required for diagnostics and establishment of food preparation technologies. For this, exhaustive protein hydrolysis with subsequent amino acid analysis is the strategy of choice. Thereby, multi-step enzymatic digestion procedures ensure good recoveries for the most of AGEs, whereas tandem mass spectrometry (MS/MS) in the multiple reaction monitoring (MRM) mode with stable isotope dilution or standard addition represents “a gold standard” for their quantification. Although the spectrum of quantitatively assessed AGE structures is continuously increases, application of untargeted profiling techniques for identification of new products is desired, especially for in vivo characterization of anti-glycative systems. Thereby, due to a high glycative potential of plant metabolites, more attention needs to be paid on plant-derived AGEs.

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“…Additionally, it provides valuable information for new quantification methods. Thus, based on the patterns of characteristic b-and y-fragments, multiple reaction monitoring (MRM)-based quantitative methods can be developed (Spiller, Frolov, and Hoffmann, 2017;. Not less importantly, synthetic peptide models provide access to valuable kinetic information.…”

Section: Plant Sciencementioning

confidence: 99%