Mining seed proteome: from protein dynamics to modification profiles

Frolov, Andrej; Мамонтова, Татьяна Васильевна; Ihling, Christian; Lukasheva, Elena; Bankin, Mikhail; Chantseva, V.V.; Vikhnina, Maria; Soboleva, Alena; Shumilina, Julia; Mavropolo-Stolyarenko, Gregory; Grishina, Tatiana; Osmolovskaya, Natalia; Zhukov, V. A.; Hoehenwarter, Wolfgang; Sinz, Andrea; Tikhononovich, Igor; Wessjohann, Ludger A.; Bilova, Tatiana; Smolikova, Galina; Медведев, С.С.

doi:10.21638/spbu03.2018.106

Cited by 20 publications

(31 citation statements)

References 100 publications

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“…In such a situation, the main negative processes within the seeds are lipid peroxidation and loss of membrane phospholipids. Meanwhile, during long-term storage of dry seeds, mostly non-enzymatic reactions would take place, and they do not require much water (Walters, 1998;Murthy et al, 2003;Veselovsky, Veselova, 2012;Frolov et al, 2018;Antonova et al, 2019). Therefore, the question on the extent to which the processes within seeds under artificial ageing would mimic those undergoing naturally during long-term storage remains unanswered (Agacka-Mołdoch et al, 2016;Bankin et al, 2018).…”

Section: Candidate Genes For Seed Longevity Identified In Barleymentioning

confidence: 99%

“…In addition to environmental factors, such as humidity, temperature, light, and absence of pathogens (Schmidt, 2000), seed ageing rates are affected by genetic factors determining seed color and weight and influencing the activity of protein non-enzymatic glycosylation processes, lipid peroxidation, cell membrane structure, generation and neutralization of reactive oxygen species (ROS) and free radicals, and other processes (Wettlaufer, Leopold, 1991;Ponquett et al, 1992;Khan et al, 1996;Wojtyla et al, 2016;Frolov et al, 2018;Antonova et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Molecular genetic bases of seed resistance to oxidative stress during storage

Швачко

Khlestkina

2020

Vestn. VOGiS

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Conservation of plant genetic diversity, including economically important crops, is the foundation for food safety. About 90 % of the world’s crop genetic diversity is stored as seeds in genebanks. During storage seeds suffer physiological stress consequences, one of which is the accumulation of free radicals, primarily reactive oxygen species (ROS). An increase in ROS leads to oxidative stress, which negatively affects the quality of seeds and can lead to a complete loss of their viability. The review summarizes data on biochemical processes that affect seed longevity. The data on the destructive effect of free radicals towards plant cell macromolecules are analyzed, and the ways to eliminate excessive ROS in plants, the most important of which is the glutathioneascorbate pathway, are discussed. The relationship between seed dormancy and seed longevity is examined. Studying seeds of different plant species revealed a negative correlation between seed dormancy and longevity, while various authors who researched Arabidopsis seeds reported both positive and negative correlations between dormancy and seed longevity. A negative correlation between seed dormancy and viability probably means that seeds are able to adapt to changing environmental conditions. This review provides a summary of Arabidopsis genes associated with seed viability. By now, a significant number of loci and genes affecting seed longevity have been identified. This review contains a synopsis of modern studies on the viability of barley seeds. QTLs associated with barley seed longevity were identified on chromosomes 2H, 5H and 7H. In the QTL regions studied, the Zeo1, Ale, nud, nadp-me, and HvGR genes were identified. However, there is still no definite answer as to which genes would serve as markers of seed viability in a certain plant species.

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Section: Candidate Genes For Seed Longevity Identified In Barleymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular genetic bases of seed resistance to oxidative stress during storage

Швачко

Khlestkina

2020

Vestn. VOGiS

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“…Legume seeds represent one of the most important sources of food protein for animal feeding and human nutrition. Among the factors, affecting the quality of this food protein, non-enzymatic post-translational modifications, like glycation, oxidation and lipoxidation, occurring both in planta [23] and after seed harvesting [37], play the most important role [38]. Due to their well-known pro-inflammatory effects, resulting advanced glycation and lipoxidation end products (AGEs and ALEs) are anti-nutritive, and essentially contribute in deterioration of plant-derived foods [39].…”

Section: Experimental Approach To Probe Pro-inflammatory Effects Of Smentioning

confidence: 99%

Drought-related Changes in the Metabolism and Nutritional Properties of Mature Pea (<em>Pisum sativum L.</em>) Seeds in the Context of Protein Glycation

Leonova¹,

Popova²,

Tsarev³

et al. 2019

Preprint

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Protein glycation is usually referred to as an array of non-enzymatic post-translational modifications, formed by reducing sugars and carbonyl products of their degradation. Resulting advanced glycation end products (AGEs) represent a heterogeneous group of covalent adducts, known for their pro-inflammatory effects in mammals, and impacting on pathogenesis of metabolic diseases and ageing. In plants, AGEs are the markers of tissue ageing and response to environmental stressors, the most prominent of which is drought. Although water deficit enhances protein glycation in leaves, its effect on seed glycation profiles is still unknown. Moreover, the effect of drought on biological activities of seed protein in mammalian systems is still unstudied in respect of glycation. Therefore, here we address the effects of a short-term drought on the patterns of seed protein-bound AGEs and accompanying alterations in pro-inflammatory properties of seed protein in the context of seed metabolome dynamics. A short-term drought, simulated as polyethylene glycol-induced osmotic stress and applied at the stage of seed filling, resulted in dramatic suppression of primary seed metabolism, although secondary metabolome was minimally affected. This was accompanied with significant suppression of NF-kB activation in human SH-SY5Y neuroblastoma cells after a treatment with protein hydrolyzates, isolated from the mature seeds of drought-treated plants. This effect could not be attributed to formation of known AGEs. Most likely, the prospective anti-inflammatory effect of short-term drought is related to antioxidant effect of unknown secondary metabolite protein adducts, or down-regulation of unknown plant-specific AGEs due to suppression of energy metabolism during seed filling.

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“…Therefore, quality of pea seeds is important from the aspects of both sustainable crop production and high nutritional efficiency. Obviously, changes in protein composition of seeds directly affect their agricultural and nutritional value [5].…”

Section: Introductionmentioning

confidence: 99%

“…Because of this, implementation of liquid chromatography (LC)-MS-based strategy is desired to get a deeper insight into the seed proteome [12]. However, as detergents, conventionally used for solubilization of protein isolates [5], dramatically affect efficiency of electrospray ionization (ESI) [13], it is difficult to find a compromise between completeness of protein reconstitution and sensitivity of MS analysis. In this context, the introduction of degradable detergents into proteomic practice helped to overcome this caveat [14].…”

Section: Introductionmentioning

confidence: 99%

Proteome Map of Pea (Pisum sativum L.) Embryos Containing Different Amounts of Residual Chlorophylls

Мамонтова

Lukasheva

Mavropolo-Stolyarenko

et al. 2018

IJMS

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Due to low culturing costs and high seed protein contents, legumes represent the main global source of food protein. Pea (Pisum sativum L.) is one of the major legume crops, impacting both animal feed and human nutrition. Therefore, the quality of pea seeds needs to be ensured in the context of sustainable crop production and nutritional efficiency. Apparently, changes in seed protein patterns might directly affect both of these aspects. Thus, here, we address the pea seed proteome in detail and provide, to the best of our knowledge, the most comprehensive annotation of the functions and intracellular localization of pea seed proteins. To address possible intercultivar differences, we compared seed proteomes of yellow- and green-seeded pea cultivars in a comprehensive case study. The analysis revealed totally 1938 and 1989 nonredundant proteins, respectively. Only 35 and 44 proteins, respectively, could be additionally identified after protamine sulfate precipitation (PSP), potentially indicating the high efficiency of our experimental workflow. Totally 981 protein groups were assigned to 34 functional classes, which were to a large extent differentially represented in yellow and green seeds. Closer analysis of these differences by processing of the data in KEGG and String databases revealed their possible relation to a higher metabolic status and reduced longevity of green seeds.

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Mining seed proteome: from protein dynamics to modification profiles

Cited by 20 publications

References 100 publications

Molecular genetic bases of seed resistance to oxidative stress during storage

Molecular genetic bases of seed resistance to oxidative stress during storage

Drought-related Changes in the Metabolism and Nutritional Properties of Mature Pea (<em>Pisum sativum L.</em>) Seeds in the Context of Protein Glycation

Proteome Map of Pea (Pisum sativum L.) Embryos Containing Different Amounts of Residual Chlorophylls

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