Quantification of quercetin and rutin from Benincasa hispida seeds and Carissa congesta roots by high-performance thin layer chromatography and high-performance liquid chromatography

Doshi, Gaurav Mahesh; Une, Hemant Devidas

doi:10.4103/0974-8490.171098

Cited by 36 publications

(18 citation statements)

References 12 publications

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“…The QCT loading was determined as previously described. 23 Briefly, QCT was extracted from the LPs with methanol; then, the QCT concentration was determined at 254 nm using high-performance liquid chromatography method with a QCT calibration curve (1.0–10 µg/mL concentration range). The calibration curve was A =0.0654C +0.0031, r =0.997.…”

Section: Methodsmentioning

confidence: 99%

RGD-modified nanoliposomes containing quercetin for lung cancer targeted treatment

Zhou

Liu

Zhao

et al. 2018

OTT

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PurposeThe aim of this study was to prepare RGD-modified nanoliposomes containing quercetin (QCT) distearoyl-L-a-phosphatidylethanolamine-polyethylene glycol 2000-RGD-liposomes ([DSPE]-PEG2000-RGD-LPs/QCT) for lung cancer targeting treatment.MethodsThe physicochemical parameters of (DSPE)-PEG2000-RGD-LPs/QCT were characterized in terms of the particle size, zeta potential, morphology, entrapment efficiency, drug loading, and in vitro release behavior. In vivo, pharmacokinetics and antitumor studies of prepared formulations were also evaluated.ResultsIn this study, QCT was found to be easily dispersed in lipid solution and entrapped by the thin-film hydration method. The encapsulation ratio and drug loading of prepared LPs were 89.2%±7.4% and 9.2%±1.3% and the mean diameter was 93.4±7.2 nm from 3 batches. The results of in vitro experiments showed that the particle size of liposomes was suitable for the fenestrated vasculatures of cancer tissues via the enhanced permeability retention effect. In vitro, a relatively slow QCT release profile was observed in (DSPE)-PEG2000-RGD-LPs, and the release mechanism fit with the Higuchi equation better. In vivo imaging results indicated that RGD-modified LPs had very good tumor targeting ability. (DSPE)-PEG2000-RGD-LPs/QCT showed a significant antitumor activity in mice with A549 tumors.ConclusionThrough this study, it was found that the RGD-modified LPs loaded with QCT could potentially be a very promising lung-targeted preparation.

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Section: Methodsmentioning

confidence: 99%

RGD-modified nanoliposomes containing quercetin for lung cancer targeted treatment

Zhou

Liu

Zhao

et al. 2018

OTT

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“…From the literature, rutin ( 60 ) and quercetin ( 63 ) are the most predominant flavonoids in Carissa [5, 6, 32, 33]. …”

Section: Chemical Constituentsmentioning

confidence: 99%

The Genus Carissa: An Ethnopharmacological, Phytochemical and Pharmacological Review

Kaunda

Zhang

2017

Nat. Prod. Bioprospect.

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Carissa L. is a genus of the family Apocynaceae, with about 36 species as evergreen shrubs or small trees native to tropical and subtropical regions of Africa, Asia and Oceania. Most of Carissa plants have been employed and utilized in traditional medicine for various ailments, such as headache, chest complains, rheumatism, oedema, gonorrhoea, syphilis, rabies. So far, only nine Carissa species have been phytochemically studied, which led to the identification of 123 compounds including terpenes, flavonoids, lignans, sterols, simple phenolic compounds, fatty acids and esters, and so on. Pharmacological studies on Carissa species have also indicated various bioactive potentials. This review covers the peer-reviewed articles between 1954 and 2016, retrieved from Pubmed, ScienceDirect, SciFinder, Wikipedia and Baidu, using “Carissa” as search term (“all fields”) and with no specific time frame set for search. Fifteen important medicinal or ornamental Carissa species were selected and summarized on their botanical characteristics, geographical distribution, traditional uses, phytochemistry, and pharmacological activities.

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“…In this study, we have targeted to achieve the presence of flavonoids and quantify the amount of active metabolite present by considering quercetin as the marker molecule. [ 16 17 ]…”

Section: Discussionmentioning

confidence: 99%

Quantification of quercetin obtained from Allium cepa Lam. leaves and its effects on streptozotocin-induced diabetic neuropathy

Dureshahwar¹,

Mubashir²,

Une³

2017

Phcog Res

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Objective:Antioxidant potential has protective effects in diabetic neuropathy (DN); hence, the present study was designed with an objective to quantify quercetin from shade-dried leaves of Allium cepa Lam. and to study its effects on streptozotocin (STZ)-induced chronic DN.Materials and Methods:The shade-dried leaves of A. cepa Lam. were extracted with methanol and then fractionated using ethyl acetate (ACEA). The quantification of quercetin in ACEA was evaluated by high-performance thin layer chromatography (HPTLC). The STZ (40 mg/kg) was administered to Sprague-Dawley rats (180–250 g) maintained at normal housing conditions. The STZ was administered once a day for 3 consecutive days. The elevation in blood glucose was monitored for 3 weeks periodically using flavin adenine dinucleotide-glucose dehydrogenase method by Contour TS glucometer. Rats showing blood glucose above 250 mg/dl were selected for the study. Animals were divided into eight groups. ACEA (25, 50, and 100 mg/kg), quercetin (40 mg/kg), metformin (120 mg/kg), and gabapentin (100 mg/kg) were given orally once a day for 2 weeks. The blood glucose level was again measured at the end of treatment to assess DN. Thermal hyperalgesia, cold allodynia, motor incoordination, and neurotoxicity were studied initially and at the end of 2-week treatment. Biochemical parameters were also evaluated after 2-week drug treatment.Results:The quercetin present in ACEA was 4.82% by HPTLC. All the ACEA treatment reduces blood glucose level at the end of the 2-week study and shows a significant neuroprotective effect in STZ-induced DN in the above experimental models.Conclusion:The quercetin present in ACEA proved protective effect in STZ-induced DN.SUMMARY High-performance thin layer chromatography reveals the presence of 4.82% quercetin in Allium cepa ethyl acetate. (ACEA). Its investigation against various diabetic neuropathy biomarkers has proved that ACEA has significant blood glucose reducing action shown neuroprotective action in thermal hyperalgesia, motor incoordination, and biochemical parameters. Abbreviations Used: HPTLC: High-performance thin layer chromatography, TLC: Thin layer chromatography, UV: Ultraviolet, ACEA: Allium cepa ethyl acetate, STZ: Streptozotocin, LDL: Low-density lipids, HDL: High-density lipids.

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Quantification of quercetin and rutin from Benincasa hispida seeds and Carissa congesta roots by high-performance thin layer chromatography and high-performance liquid chromatography

Cited by 36 publications

References 12 publications

RGD-modified nanoliposomes containing quercetin for lung cancer targeted treatment

RGD-modified nanoliposomes containing quercetin for lung cancer targeted treatment

The Genus Carissa: An Ethnopharmacological, Phytochemical and Pharmacological Review

Quantification of quercetin obtained from Allium cepa Lam. leaves and its effects on streptozotocin-induced diabetic neuropathy

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