Objective:Antioxidant potential has protective effects in diabetic neuropathy (DN); hence, the present study was designed with an objective to quantify quercetin from shade-dried leaves of Allium cepa Lam. and to study its effects on streptozotocin (STZ)-induced chronic DN.Materials and Methods:The shade-dried leaves of A. cepa Lam. were extracted with methanol and then fractionated using ethyl acetate (ACEA). The quantification of quercetin in ACEA was evaluated by high-performance thin layer chromatography (HPTLC). The STZ (40 mg/kg) was administered to Sprague-Dawley rats (180–250 g) maintained at normal housing conditions. The STZ was administered once a day for 3 consecutive days. The elevation in blood glucose was monitored for 3 weeks periodically using flavin adenine dinucleotide-glucose dehydrogenase method by Contour TS glucometer. Rats showing blood glucose above 250 mg/dl were selected for the study. Animals were divided into eight groups. ACEA (25, 50, and 100 mg/kg), quercetin (40 mg/kg), metformin (120 mg/kg), and gabapentin (100 mg/kg) were given orally once a day for 2 weeks. The blood glucose level was again measured at the end of treatment to assess DN. Thermal hyperalgesia, cold allodynia, motor incoordination, and neurotoxicity were studied initially and at the end of 2-week treatment. Biochemical parameters were also evaluated after 2-week drug treatment.Results:The quercetin present in ACEA was 4.82% by HPTLC. All the ACEA treatment reduces blood glucose level at the end of the 2-week study and shows a significant neuroprotective effect in STZ-induced DN in the above experimental models.Conclusion:The quercetin present in ACEA proved protective effect in STZ-induced DN.SUMMARY High-performance thin layer chromatography reveals the presence of 4.82% quercetin in Allium cepa ethyl acetate. (ACEA). Its investigation against various diabetic neuropathy biomarkers has proved that ACEA has significant blood glucose reducing action shown neuroprotective action in thermal hyperalgesia, motor incoordination, and biochemical parameters. Abbreviations Used: HPTLC: High-performance thin layer chromatography, TLC: Thin layer chromatography, UV: Ultraviolet, ACEA: Allium cepa ethyl acetate, STZ: Streptozotocin, LDL: Low-density lipids, HDL: High-density lipids.
Background: Neuropathy can be induced in rats by peripheral injuries, depending on compression of complete or a section of sciatic nerve and chemically by Streptozotocin (STZ). Materials and Methods: In the present study neuropathic pain were induced in rats by two methods, chronic constriction injury (surgical model) and STZ (40mg/kg/i.p.) induced diabetes (drug induced model). In both the models, behavioural as well as markers of oxidative stress were studied. Behavioural parameters were tested using vonfrey hair and Randall Selitto analgesiometer whereas biochemical parameter includes glycosylated haemoglobin and markers of oxidative stress. The study was further supported by histopathology of sciatic nerve. Flavonoid rich extract of Allium cepa Lam. leaves was administered at three different doses viz. 25, 50 and 100mg/kg/p.o to the rats with neuropathic pain. Both the models of neuropathic pain showed significant alteration in behavioural as well as oxidative stress parameters. Results: Treatment of Allium cepa leaves extract showed dose dependent improvement in behavioural and biochemical parameters towards normal (p value <0.001, <0.05 and <0.01). The altered histopathological changes in sciatic nerve were also significantly improved as compared to rats with neuropathic pain. Conclusion: The neuroprotective effects of the Allium cepa leaves extract is a virtue of its strong antioxidant activity.
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