Structured Abstract
Objectives:
The aim is to highlight a novel three-dimensional (3D) imaging methodology using micro-CT scans to visualize and measure bone modelling in an animal model. In order to validate the new methodology, we compared the 3D imaging method to traditional two-dimensional (2D) histomorphometry to assess growth changes in the jaws of a rodent.
Setting and sample population:
Rodent animal models.
Material and methods:
Eleven rats were obtained from a larger previously published study. Sixty undecalcified histological sections from the maxilla and corresponding high-resolution in vivo micro-CT reconstructions were obtained. Bone modelling changes on specific alveolar surfaces were measured using traditional histomorphometry Measurements of bone growth were also obtained via 3D Slicer software from 3D micro-CT generated models from the same plane containing the histological images. Both qualitative and quantitative 3D methods were compared to traditional histological measurements. Quantitative agreement between methods was categorized as follows: poor (>150 μm), good (150–100 μm) and excellent (<100 μm).
Results:
Both qualitative (88.3%) and quantitative (86.7%) 3D measurements showed excellent agreement, when compared to histomorphometric measurements. Only 1.7% and 5% of the comparisons exhibited poor agreement (>150 μm) for qualitative and quantitative methods, respectively.
Discussion:
The new 3D superimposition method compares very favourably with traditional histology. It is likely that in the future, such methods will be used in studies of bone adaptation.
Conclusion:
The 3D micro-CT qualitative and quantitative methods are reliable for measuring bone modelling changes and compare favourably to histology for the specific application described.