Quantification of DOX bioavailability in biological samples of mice by sensitive and precise HPLC assay

Zhang, Chan; Wu, Yifan; Dong, Yuefeng; Xu, Huibin; Li, Zhao

doi:10.3109/13880209.2015.1014918

Cited by 16 publications

(12 citation statements)

References 23 publications

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“…The low fluorescence intensity in the main organs indicated that free DOX could be excreted quickly from the body. This result verified the short half-life and rapid clearance of DOX 42 43 44 . It is also noted that the fluorescence intensity in tumor for free DOX group was greatly reduced after injection for 8 h and 24 h, respectively, indicating the low retention of free DOX in tumor.…”

Section: Resultssupporting

confidence: 78%

Co-delivery of doxorubicin and curcumin by pH-sensitive prodrug nanoparticle for combination therapy of cancer

Zhang

Yang

Wang

et al. 2016

Sci Rep

196

124

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Ample attention has focused on cancer drug delivery via prodrug nanoparticles due to their high drug loading property and comparatively lower side effects. In this study, we designed a PEG-DOX-Cur prodrug nanoparticle for simultaneous delivery of doxorubicin (DOX) and curcumin (Cur) as a combination therapy to treat cancer. DOX was conjugated to PEG by Schiff’s base reaction. The obtained prodrug conjugate could self-assemble in water at pH 7.4 into nanoparticles (PEG-DOX NPs) and encapsulate Cur into the core through hydrophobic interaction (PEG-DOX-Cur NPs). When the PEG-DOX-Cur NPs are internalized by tumor cells, the Schiff’s base linker between PEG and DOX would break in the acidic environment that is often observed in tumors, causing disassembling of the PEG-DOX-Cur NPs and releasing both DOX and Cur into the nuclei and cytoplasma of the tumor cells, respectively. Compared with free DOX, free Cur, free DOX-Cur combination, or PEG-DOX NPs, PEG-DOX-Cur NPs exhibited higher anti-tumor activity in vitro. In addition, the PEG-DOX-Cur NPs also showed prolonged blood circulation time, elevated local drug accumulation and increased tumor penetration. Enhanced anti-tumor activity was also observed from the PEG-DOX-Cur-treated animals, demonstrating better tumor inhibitory property of the NPs. Thus, the PEG-DOX-Cur prodrug nanoparticle system provides a simple yet efficient approach of drug delivery for chemotherapy.

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Section: Resultssupporting

confidence: 78%

Co-delivery of doxorubicin and curcumin by pH-sensitive prodrug nanoparticle for combination therapy of cancer

Zhang

Yang

Wang

et al. 2016

Sci Rep

196

124

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“…Optimal chromatographic conditions for determining the analyte in biological specimens should offer efficient separation of the analyte from other exogenous components, a favorable mobile phase with simple composition and appropriate pH value, a concise and efficient procedure, and excellent accuracy and precision [15]. …”

Section: Discussionmentioning

confidence: 99%

“…Leaky capillaries present in the inflamed synovium and tumor tissue share some unique characteristics that allow liposomal drug formulations to extravasate into the inflammatory/tumor tissues and be retained in the extravascular space [10,11]. Targeted accumulation of liposomal drug formulations in tumor tissue has been previously demonstrated [13–15], whereas similar research on delivery of liposomal drug formulations to inflamed RA/CIA joints is rare. To the best of our knowledge, we quantified the amount of liposomal drug formulations in arthritic joints by using HPLC for the first time.…”

Section: Discussionmentioning

confidence: 99%

High-Performance Liquid Chromatography (HPLC) Quantification of Liposome-Delivered Doxorubicin in Arthritic Joints of Collagen-Induced Arthritis Rats

Niu

et al. 2017

Med Sci Monit Basic Res

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BackgroundNeoangiogenesis occurring in inflamed articular synovium in early rheumatoid arthritis (RA) is characterized by enhanced vascular permeability that allows nanoparticle agents, including liposomes, to deliver encapsulated drugs to arthritic joints and subsequently improve therapeutic efficacy and reduce adverse effects. However, the targeting distribution of liposomes in arthritic joints during RA has not been quantitatively demonstrated. We performed this study to evaluate the targeting distribution of PEGylated doxorubicin liposomes in the arthritic joints of collagen-induced arthritis (CIA) rats by high-performance liquid chromatography (HPLC).Material/MethodsTwo doxorubicin formulations were administered to CIA rats via tail intravenous injection at a single dose (50 mg/m2). CIA rats were sacrificed and the tissues of the inflamed ankle joints were collected. The content of doxorubicin in the arthritic joints was analyzed by a validated and reproducible HPLC method. A two-way ANOVA for 2×5 factorial design was used for statistical analysis.ResultsThe developed HPLC method was sensitive, precise, and reproducible. The method was successfully applied to quantify doxorubicin content in arthritic tissues. At each time point (6, 12, 24, 48, and 72 h), doxorubicin content in the arthritic joints of the doxorubicin liposome group (DOX-LIP group) was higher than in the free doxorubicin group (DOX group) (P<0.05). In the DOX-LIP group, doxorubicin levels in the arthritic joints increased gradually and significantly in the interval of 6–72 h post-administration.ConclusionsPEGylated doxorubicin liposomes were targeted to, accumulated, and retained in the arthritic joints of CIA rats. The present study indicates that liposome encapsulation increases the therapeutic efficacy of antirheumatic drugs, presenting a promising therapeutic strategy for RA.

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“…The drug loading content (DLC) of CCM/SLI/R-D was determined by emerging the as-prepared nanocarriers in methanol for 48 h. The samples were centrifuged (10,000 rpm, 30 min), and the supernatants were collected for the determination of DOX by high-performance liquid chromatography (HPLC) [33]. The miR495 loading was determined by UV absorbance at 260 nm (UV5Nano, METTLER TOLEDO, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

Cancer Cell Membrane Decorated Silica Nanoparticle Loaded with miR495 and Doxorubicin to Overcome Drug Resistance for Effective Lung Cancer Therapy

Gong

Qin

et al. 2019

Nanoscale Res Lett

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Current cancer therapy usually succumbs to many extracellular and intracellular barriers, among which untargeted distribution and multidrug resistance (MDR) are two important difficulties responsible for poor outcome of many drug delivery systems (DDS). Here, in our study, the dilemma was addressed by developing a cancer cell membrane (CCM)-coated silica (SLI) nanoparticles to co-deliver miR495 with doxorubicin (DOX) for effective therapy of lung cancer (CCM/SLI/R-D). The homologous CCM from MDR lung cancer cells (A549/DOX) was supposed to increase the tumor-homing property of the DDS to bypass the extracellular barriers. Moreover, the MDR of cancer cells were conquered through downregulation of P-glycoprotein (P-gp) expression using miR495. It was proved that miR495 could significantly decrease the expression of P-gp which elevated intracellular drug accumulation in A549/DOX. The in vitro and in vivo results exhibited that CCM/SLI/R-D showed a greatly enhanced therapeutic effect on A549/DOX, which was superior than applying miR495 or DOX alone. The preferable effect of CCM/SLI/R-D on conquering the MDR in lung cancer provides a novel alternative for effective chemotherapy of MDR cancers.

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Quantification of DOX bioavailability in biological samples of mice by sensitive and precise HPLC assay

Cited by 16 publications

References 23 publications

Co-delivery of doxorubicin and curcumin by pH-sensitive prodrug nanoparticle for combination therapy of cancer

Co-delivery of doxorubicin and curcumin by pH-sensitive prodrug nanoparticle for combination therapy of cancer

High-Performance Liquid Chromatography (HPLC) Quantification of Liposome-Delivered Doxorubicin in Arthritic Joints of Collagen-Induced Arthritis Rats

Cancer Cell Membrane Decorated Silica Nanoparticle Loaded with miR495 and Doxorubicin to Overcome Drug Resistance for Effective Lung Cancer Therapy

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