Quantification of doripenem in human plasma and peritoneal fluid by high-performance liquid chromatography with ultraviolet detection

Ikeda, Kayo; Ikawa, Kazuro; Morikawa, Norifumi; Kameda, Keiko; Urakawa, Nami; Ohge, Hiroki; Sueda, Taijiro

doi:10.1016/j.jchromb.2008.03.001

Cited by 39 publications

(44 citation statements)

References 12 publications

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“…[7][8][9][10] Other studies include the quantitation of doripenem in biological fluids. 11,12 For other carbapenems, the works also focus on quantitation by LC, UV spectrophotometry and microbiological assay, applied on stability studies. [13][14][15] For meropenem, Almarsson et al 16 reports the analysis of the drug by proton and 13-carbon NMR coupled with tandem mass spectroscopy, which showed evidence that the structure is stable as CO 2 adduct, in solid state and in solution.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the antibiotic doripenem using physicochemical methods: chromatography, spectrophotometry, spectroscopy and thermal analysis

et al. 2011

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Recebido em 1/11/10; aceito em 14/5/11; publicado na web em 22/7/11 Doripenem was characterized through physicochemical and spectroscopic techniques, as well as thermal analysis. TLC (Rf = 0.62) and HPLC (rt = 7.4 min) were found to be adequate to identify the drug. UV and infrared spectra showed similar profile between doripenem bulk and standard. The 1 H and 13 C NMR analysis revealed chemical shifts that allowed identifying the drug. Thermal analysis demonstrated three steps with mass loss, at 128, 178 and 276 o C. The work was successfully applied to qualitative analysis of doripenem, showing the reported methods can be used for physicochemical characterization of doripenem.

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Section: Introductionmentioning

confidence: 99%

Characterization of the antibiotic doripenem using physicochemical methods: chromatography, spectrophotometry, spectroscopy and thermal analysis

et al. 2011

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“…DRPM Assay Total DRPM concentration in plasma was measured according to the HPLC method described by Ikeda et al 13) with modification. Briefly, 50 µL of 100 µg/mL meropenem in extra pure water (internal standard) and 400 µL of extra pure water were added to 20 µL of plasma in a polyethylene centrifuge tube.…”

Section: Subjectsmentioning

confidence: 99%

Pharmacokinetic/Pharmacodynamic Analysis for Doripenem Regimens in Intensive Care Unit Patient

Tanaka

Sato

Goto

et al. 2017

Biological & Pharmaceutical Bulletin

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Doripenem (DRPM) is a broad-spectrum antibacterial agent often used as empirical therapy for critically ill patients, although there is a lack of studies validating the recommended dosage regimen for patients admitted to intensive care unit (ICU), based on pharmacokinetic (PK)/pharmacodynamic (PD) index. In this study, we estimated the free time above minimum inhibitory concentration (fT>MIC (%)) of DRPM using population PK analysis of 12 patients in ICU, and evaluated the validity of the dosage regimen stratified by creatinine clearance. Using a 2-compartment population PK model reported previously, the mean total clearance or distribution volume of DRPM estimated by Bayesian estimation was significantly lower or higher than that of based on population PK model. The estimated fT>MIC (%) of the recommended standard (normal renal function: 0.5 g every 8 h, moderate: 0.25 g every 8 h, severe renal impairment: 0.25 g every 12 h) and higher doses (normal: 1.0 g every 8 h, moderate: 0.5 g every 8 h, severe: 0.25 g every 8 h) against MICs of 0.5, 1 and 2 µg/mL exceeded 40% in all patients. When stratified by creatinine clearance, the PK/PD breakpoints estimated by Monte Carlo simulation in three grades of renal function tended to be higher than the previously reported PK/PD breakpoints for patients with urinary tract infection, an infection of lesser severity than ICU patients. These results suggest that the dosage regimen stratified by renal function derived from Japanese package insert may be sufficient to achieve effective treatment in ICU patients.Key words doripenem; pharmacokinetic/pharmacodynamic analysis; intensive care unit; breakpoint Doripenem (DRPM) is a newer carbapenem antimicrobial agent with potent and broad spectrum activities against Grampositive, Gram-negative and anaerobic bacteria, and is more potent against Pseudomonas aeruginosa than other carbapenems.1-3) Doses of 0.5-3.0 g daily in two or three divided doses administered by 0.5 or 1 h infusion are used commonly to treat infectious diseases such as pneumonia, complicated urinary tract infection and intra-abdominal infections. In order to provide optimal antimicrobial therapy, selecting an effective and well-tolerated dosage regimen based on pharmacokinetic (PK)/ pharmacodynamic (PD) index is important. For DRPM and other β-lactams with time-dependent antimicrobial activity, an effective dosage regimen requires that the blood concentrations exceed the minimum inhibitory concentration (MIC) of the infecting bacteria for at least 40% of the dosing interval.4) Therefore, the appropriate dosage regimen based on time above MIC (%T>MIC) is of great importance when using DRPM.Critically ill patients in the intensive care unit (ICU) are often affected by infections, and are given antimicrobial agents with a broad spectrum. 5) In particular, since severe sepsis and septic shock are major causes of morbidity and mortality in the ICU, early use of carbapenems including DRPM as an empirical therapy is recommended by international guidelines for the manageme...

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“…DP and MP were broad spectrum of antimicrobial activity against both gram-positive and gram-negative bacteria, including pseudomonas aeruginosa [3][4][5].The catalytic effect of citrate, phosphate and acetate buffers on the degradation of imipenem has been studied [6]. Several methods have been reported in the literature for the determination of doripenem and meropenem and its metabolites in biological fluids and pharmaceutical formulation using different techniques, including spectrophotometry, high performance liquid chromatography (HPLC) and liquid chromatography methods [7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Graphene and Polyaniline Composite Modified Glassy Carbon Electrode for Electrochemical Determination of Doripenem and Meropenem Metabolites

Sivaprasad¹

2014

J Anal Bioanal Tech

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Harrison et al. [22] studied the metabolism of meropenem and *Corresponding author: Sreedhar NY, Electroanalytical Lab, Department of Chemistry, Sri Venkateswara University, Tirupati-517502, Andhra Pradesh, India, Tel: +91-9440087124; E-mail: sreedharny.chem@gmail.com AbstractThe theme of this study was the preparation, characterization, and application of a polyaniline (PAN) and graphene composite modified glassy carbon electrode (PAN/Gr/GCE) for the voltammetric determination of doripenem (DPM) and meropenem metabolites (MPM) in human urine and serum samples. The morphological study of the PAN/ Gr/GCE composite was examined by scanning electron microscopy (SEM) and cyclic voltammetry (CV). The electrochemical behaviour of DPM and MPM at the PAN/Gr/GCE were investigated using cyclic voltammetry in Ag/ AgCl/KCl supporting electrolyte at pH 2.0-10.0 in phosphate buffer solution. The linear dependence of current versus concentration was reached in a wide concentration range from 2.5×10 -7 M to 3.5×10 -4 M using cyclic voltammetry and differential pulse voltammetric methods. In acidic media a non-reversible diffusion controlled reduction involving two protons and two electrons occurs at carbon and nitrogen double bond (C=N) in the metabolites. The best electroanalytical performances of this composite electrode were achieved with the detection limits 3.6×10 -9 M and 1.75×10 -9 M for DPM and MPM respectively. The simplicity of preparation, high sensitivity and stability of this composite electrode should open novel avenues and applications for fabricating robust sensors for detection of DPM and MPM in human urine and serum samples.

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Quantification of doripenem in human plasma and peritoneal fluid by high-performance liquid chromatography with ultraviolet detection

Cited by 39 publications

References 12 publications

Characterization of the antibiotic doripenem using physicochemical methods: chromatography, spectrophotometry, spectroscopy and thermal analysis

Characterization of the antibiotic doripenem using physicochemical methods: chromatography, spectrophotometry, spectroscopy and thermal analysis

Pharmacokinetic/Pharmacodynamic Analysis for Doripenem Regimens in Intensive Care Unit Patient

Graphene and Polyaniline Composite Modified Glassy Carbon Electrode for Electrochemical Determination of Doripenem and Meropenem Metabolites

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