Quality evaluation of Rhizoma Belamcandae (Belamcanda chinensis (L.) DC.) by using high-performance liquid chromatography coupled with diode array detector and mass spectrometry

Jun, Li; Li, Winnie Z.M.; Huang, Wen; Cheung, Anna Wing Han; Bi, Cathy W. C.; Duan, Ran; Guo, Ava J.Y.; Dong, Tina Tingxia; Tsim, Karl Wah Keung

doi:10.1016/j.chroma.2008.05.082

Cited by 70 publications

(39 citation statements)

References 26 publications

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“…The process of standardization included the selection of "common peaks" in chromatograms and the normalization of retention times of all the common peaks. 24) To standardize the fingerprint, 10 samples from various locations were analyzed with the developed procedure. Peaks existing in all batches of samples were assigned as "common peaks" for CR.…”

Section: Hplc-uv Fingerprint Analysismentioning

confidence: 99%

Fingerprint Analysis and Simultaneous Determination of Phenolic Compounds in Extracts of Curculiginis Rhizoma by HPLC-Diode Array Detector

et al. 2013

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Curculiginis Rhizoma (Curculigo orchioides Gaertn.) is a well-known Chinese herbal medicine, as well as an important Rasayana drug in India. Current criteria of quality control on this herb are to quantitatively analyze single compound curculigoside, which fail to comprehensively evaluate quality of this herb. In this paper, a simple and reliable HPLC coupled with diode array detector (DAD) method was developed to evaluate the quality of Curculiginis Rhizoma through establishing chromatographic fingerprint and simultaneously quantitating four phenolic compounds, orcinol glucoside, orcinol, 2,6-dimethoxybenzoic acid and curculigoside. The fingerprint displayed eleven common peaks, and the similarity index of different samples was in a range of 0.890-0.977. Validation of the method was acceptable, with 96.03-102.82% accuracy in recovery test and inter and intra-day precisions were less than 2%. This developed method by having a combination of chromatographic fingerprint and quantitation analysis could be applied to the quality control of Curculiginis Rhizoma.

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Section: Hplc-uv Fingerprint Analysismentioning

confidence: 99%

Fingerprint Analysis and Simultaneous Determination of Phenolic Compounds in Extracts of Curculiginis Rhizoma by HPLC-Diode Array Detector

et al. 2013

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“…This substance usually occurs as a glucoside and is also found in a variety of other plant families, including Anemmarhena asphodeloides [3], Bersama abyssinica [4], Cyclopia genistoides [5], Cyclopia subternata [6], Gentiana lutea [7], Gnidia involucrata [8], Rhizoma belamcandae [9] and Salacia oblonga [10], among others. Notably, M. indica and several of the above listed plants have been traditionally used to treat important human diseases, such as diabetes [10], obesity [10], cancer [11], and asthma [12].…”

Section: Introductionmentioning

confidence: 99%

Mangiferin Prevents Guinea Pig Tracheal Contraction via Activation of the Nitric Oxide-Cyclic GMP Pathway

et al. 2013

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Previous studies have described the antispasmodic effect of mangiferin, a natural glucoside xanthone (2-C-β-Dgluco-pyranosyl-1,3,6,7-tetrahydroxyxanthone) that is present in mango trees and other plants, but its mechanism of action remains unknown. The aim of this study was to examine the potential contribution of the nitric oxide-cyclic GMP pathway to the antispasmodic effect of mangiferin on isolated tracheal rings preparations. The functional effect of mangiferin on allergic and non-allergic contraction of guinea pig tracheal rings was assessed in conventional organ baths. Cultured tracheal rings were exposed to mangiferin or vehicle, and nitric oxide synthase (NOS) 3 and cyclic GMP (cGMP) levels were quantified using western blotting and enzyme immunoassays, respectively. Mangiferin (0.1–10 µM) inhibited tracheal contractions induced by distinct stimuli, such as allergen, histamine, 5-hydroxytryptamine or carbachol, in a concentration-dependent manner. Mangiferin also caused marked relaxation of tracheal rings that were precontracted by carbachol, suggesting that it has both anti-contraction and relaxant properties that are prevented by removing the epithelium. The effect of mangiferin was inhibited by the nitric oxide synthase inhibitor, Nω-nitro-L-arginine methyl ester (L-NAME) (100 µM), and the soluble guanylate cyclase inhibitor, 1H-[1], [2], [4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) (10 µM), but not the adenylate cyclase inhibitor, 9-(tetrahydro-2-furyl)adenine (SQ22536) (100 µM). The antispasmodic effect of mangiferin was also sensitive to K+ channel blockers, such as tetraethylammonium (TEA), glibenclamide and apamin. Furthermore, mangiferin inhibited Ca2+-induced contractions in K+ (60 mM)-depolarised tracheal rings preparations. In addition, mangiferin increased NOS3 protein levels and cGMP intracellular levels in cultured tracheal rings. Finally, mangiferin-induced increase in cGMP levels was abrogated by co-incubation with either ODQ or L-NAME. These data suggest that the antispasmodic effect of mangiferin is mediated by epithelium-nitric oxide- and cGMP-dependent mechanisms.

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“…Pharmacological investigations have demonstrated that isoflavones could be major bioactive constituents of Rhizoma Belamcandae [2][3][4][5]. Among them, tectoridin, tectorigenin, iridin, irigenin and irisflorentin are the most abundant active ingredients [6]. Tectoridin and iridin are the prodrugs of tectorigenin and irigenin, respectively.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of tectorigenin, irigenin and irisflorentin in rat plasma and urine by UHPLC–MS/MS: Application to pharmacokinetics

Zhang¹,

Yang²,

Wang³

et al. 2011

Journal of Chromatography B

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Quality evaluation of Rhizoma Belamcandae (Belamcanda chinensis (L.) DC.) by using high-performance liquid chromatography coupled with diode array detector and mass spectrometry

Cited by 70 publications

References 26 publications

Fingerprint Analysis and Simultaneous Determination of Phenolic Compounds in Extracts of Curculiginis Rhizoma by HPLC-Diode Array Detector

Fingerprint Analysis and Simultaneous Determination of Phenolic Compounds in Extracts of Curculiginis Rhizoma by HPLC-Diode Array Detector

Mangiferin Prevents Guinea Pig Tracheal Contraction via Activation of the Nitric Oxide-Cyclic GMP Pathway

Simultaneous determination of tectorigenin, irigenin and irisflorentin in rat plasma and urine by UHPLC–MS/MS: Application to pharmacokinetics

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