Fingerprint Analysis and Simultaneous Determination of Phenolic Compounds in Extracts of Curculiginis Rhizoma by HPLC-Diode Array Detector

Abstract

Curculiginis Rhizoma (Curculigo orchioides Gaertn.) is a well-known Chinese herbal medicine, as well as an important Rasayana drug in India. Current criteria of quality control on this herb are to quantitatively analyze single compound curculigoside, which fail to comprehensively evaluate quality of this herb. In this paper, a simple and reliable HPLC coupled with diode array detector (DAD) method was developed to evaluate the quality of Curculiginis Rhizoma through establishing chromatographic fingerprint and simultaneously quantitating four phenolic compounds, orcinol glucoside, orcinol, 2,6-dimethoxybenzoic acid and curculigoside. The fingerprint displayed eleven common peaks, and the similarity index of different samples was in a range of 0.890-0.977. Validation of the method was acceptable, with 96.03-102.82% accuracy in recovery test and inter and intra-day precisions were less than 2%. This developed method by having a combination of chromatographic fingerprint and quantitation analysis could be applied to the quality control of Curculiginis Rhizoma.

Biomolecules and Natural Medicine Preparations: Analysis of New Sources of Bioactive Compounds from Ribes and Rubus spp. Buds

“…The obtained fingerprints, and relative phytocomplexes, were useful for authentication and quality control purposes, as shown in other studies [ 40 , 41 ]. Most of the research pointed out that the identified antioxidant compounds (polyphenols and vitamins) contribute significantly to the total phytocomplex of herbal preparations [ 31 , 42 ]: the present study confirmed these results, adding as well as the terpenic and organic compounds also significantly contributed to the bud-preparation phytocomplex, as anti-inflammatory and volatile constituents in herbal preparations.…”

Phytochemical fingerprint and chemometrics for natural food preparation pattern recognition: an innovative technique in food supplement quality control

“…Fast screening techniques and simple sample preparation are growing priorities intended to make methods more convenient and cost-effective: chromatographic fingerprint, a more significant formulation for controlling the quality of herbal medicines and their products, has been accepted by many countries and organizations. To provide a fingerprint, the entire chromatogram may be used as a chemical profile, or a chromatographic discriminating region or a selection of significant chemical markers: in this study, all the chromatographic pattern was used for fingerprint evaluation, according to other similar studies (Bian et al 2013).…”

Applications of High Performance Liquid Chromatography in the Analysis of Herbal Products

“…Curculiginis Rhizoma, the dry rhizome of C. orchioides, is a well-known Chinese herbal medicinal product and also an important Ayurvedic product used for the treatment of pain, inflammation, immune and hepatic disorders, and as an aphrodisiac [79]. Curculigoside, a phenolic glycoside, is considered to be the major contributor to the medicinal properties of this herbal product.…”