Simultaneous determination of tectorigenin, irigenin and irisflorentin in rat plasma and urine by UHPLC–MS/MS: Application to pharmacokinetics

Zhang, Weidong; Yang, Wu; Wang, Xiaojuan; Gu, Yongan; Wang, Rong

doi:10.1016/j.jchromb.2011.10.022

Cited by 23 publications

(11 citation statements)

References 10 publications

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“…The time points of pharmacokinetic study were designed to 36 h, in combination with the preliminary test results that the plasma concentrations of caffeic acid and quinic acid at 24 h remained high. And the experiments prove that the project is more reasonable than that of only within 24 h. The T max values of compounds I–IV concentrations were achieved in 0.25, 0.25, 1.46, and 6.00 h and the t 1/2 ranged from 4.95 to 8.45 h. It was suggested that the entrance of chicoric acid and chlorogenic acid in blood was through absorption in the stomach . The concentration–time curves of cichoric acid and chlorogenic acid exhibited a relatively rapid absorption process, of which the plasma concentration achieved the peak from 15 to 30 min and showed a relatively sharp peak shape.…”

Section: Resultsmentioning

confidence: 78%

Simultaneous determination and pharmacokinetic study of four phenol compounds in rat plasma by ultra-high performance liquid chromatography with tandem mass spectrometry after oral administration of Echinacea purpurea extract

Gan

Liu

et al. 2016

J. Sep. Science

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A rapid and sensitive assay based on ultra-high performance liquid chromatography with electrospray ionization tandem mass spectrometry was established and validated for the simultaneous determination of cichoric acid, chlorogenic acid, quinic acid, and caffeic acid in rat plasma after oral administration of Echinacea purpurea extract using butylparaben as the internal standard. Samples were pretreated by liquid-liquid extraction with ethyl acetate. The separations for analytes were performed on an ACQUITY UPLC HSS C18 column (1.8 μm 2.1 × 100 mm) using a gradient elution program with acetonitrile/10 mM ammonium acetate (pH 5.6) at a flow rate of 0.3 mL/min. The analytes were detected in multiple reaction monitoring mode with negative electrospray ionization. The lower limit of quantification of each analyte was not higher than 10.85 ng/mL. The relative standard deviation of the intraday and interday precisions was less than 14.69%. The relative errors of accuracies were in the range of -13.80 to 14.91%. The mean recoveries for extraction recovery and matrix effect were higher than 80.79 and 89.98%, respectively. The method validation results demonstrated that the proposed method was sensitive, specific, and reliable, which was successfully applied to the pharmacokinetic study of four components after oral administration of Echinacea purpurea extract.

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Section: Resultsmentioning

confidence: 78%

Simultaneous determination and pharmacokinetic study of four phenol compounds in rat plasma by ultra-high performance liquid chromatography with tandem mass spectrometry after oral administration of Echinacea purpurea extract

Gan

Liu

et al. 2016

J. Sep. Science

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“…In contrast with corynoline, 8‐oxocorynoline contains a carbonyl, which might lead to keto–enol tautomerism . It could easily form a conjugate with glucuronide and sulfate, which results in a difference in its biotransformation in vivo by intestinal bacteria and enzymes . These results should be helpful for further studies on pharmacokinetics, pharmacy, and toxicity of the TCM C. bungeana Turcz and should promote research on its efficacy in clinical studies.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous determination and pharmacokinetics of five alkaloids in rat plasma by ultra high performance liquid chromatography with tandem mass spectrometry after the oral administration of Corydalis bungeana Turcz extract

Liu

Wang

et al. 2015

J of Separation Science

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Pharmacokinetic studies were conducted on rats for protopine, corynoline, 7'-(3',4'-dihydroxyphenyl)-N-[(4-methoxyphenyl)-ethyl]propenamide, acetylcorynoline, and 8-oxocorynoline, five main active components from Corydalis bungeana Turcz (C. bungeana Turcz). An ultra high performance liquid chromatography with tandem mass spectrometry method has been developed and validated for the simultaneous determination of the pharmacokinetic characteristics of these components in rat plasma. Chromatographic separation was achieved on an Agilent SB-C18 column (1.8 μm, 150 × 2.1 mm) using a gradient elution program with a mobile phase consisting of acetonitrile and water containing 0.1% acetic acid at a flow rate of 0.3 mL/min. The analytes were detected in the multiple reaction monitoring mode with positive electrospray ionization. Lower limits of quantification were >0.680 ng/mL and matrix effects ranged from 91.26 to 100.38%. The mean extraction recoveries of quality control samples were less than 79.32%, and the precision and accuracy were within the acceptable limits. All analytes were proven to be stable during sample storage and analysis procedures. The validated method was successfully applied to the pharmacokinetic study of five alkaloid components after oral administration of C. bungeana Turcz extract to rats. The obtained results may be helpful to reveal the mechanism of action and to guide the clinical application of C. bungeana Turcz.

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“…Irigenin is an active ingredient of the herbal medicine Rhizoma Belamcanda, which is officially listed in the Chinese pharmacopoeia and is widely used 45 . Considering its specificity in targeting the FN-EDA C-Cʹ loop 37 and its known safety as a bioactive constituent of an officiallyapproved drug, irigenin could be a potential anti- shown as mean ± SEM; pFAK: FN-EDA versus Irigenin+FN-EDA: **P < 0.001, FN-EDA versus Ctrl: **P < 0.001; pSrc: FN-EDA versus Irigenin+FN-EDA: **P < 0.001, FN-EDA versus Ctrl: **P < 0.001.)…”

Section: Blocking Of Eda/integrin Combination Suppresses Hepatic Angimentioning

confidence: 99%

FN-EDA mediates angiogenesis of hepatic fibrosis via integrin-VEGFR2 in a CD63 synergetic manner

Xie

et al. 2020

Cell Death Discov.

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Pathological angiogenesis is an important component of hepatic fibrosis along with fibrous deposition, but its role is not well understood. Here, we demonstrated that fibronectin containing extra domain A(FN-EDA), a fibronectin splice variant highly expressed in hepatic fibrosis, mediated angiogenesis in disease progression. FN-EDA was positively correlated with pathological angiogenesis in hepatic fibrosis, and a reduction in FN-EDA expression was associated with diminished intrahepatic angiogenesis and fibrosis. FN-EDA mostly colocalized with hepatic stellate cells (HSCs) and interference or blockage of FN-EDA attenuated migration and tube formation in co-cultured endothelial cells. Mechanistic studies indicated that FN-EDA was secreted to promote phosphorylation of VEGFR2 with the assistance of integrin and CD63. Targeting FN-EDA-integrin combination postponed the progression of hepatic angiogenesis and fibrosis in vivo. These results indicated that FN-EDA plays an emerging role in angiogenesis in hepatic fibrosis and could be a potential therapeutic intervention for the disease.

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Simultaneous determination of tectorigenin, irigenin and irisflorentin in rat plasma and urine by UHPLC–MS/MS: Application to pharmacokinetics

Cited by 23 publications

References 10 publications

Simultaneous determination and pharmacokinetic study of four phenol compounds in rat plasma by ultra-high performance liquid chromatography with tandem mass spectrometry after oral administration of Echinacea purpurea extract

Simultaneous determination and pharmacokinetic study of four phenol compounds in rat plasma by ultra-high performance liquid chromatography with tandem mass spectrometry after oral administration of Echinacea purpurea extract

Simultaneous determination and pharmacokinetics of five alkaloids in rat plasma by ultra high performance liquid chromatography with tandem mass spectrometry after the oral administration of Corydalis bungeana Turcz extract

FN-EDA mediates angiogenesis of hepatic fibrosis via integrin-VEGFR2 in a CD63 synergetic manner

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