Quality control measures in an embryo research program

Three year results of in vitro production of bovine embryos in serum-poor bovine oviduct conditioned medium. An overview

“…Unfavourable ambient conditions may have been a factor in the relatively low results obtained in Guelph, where shortly after these experiments were completed, the control co-culture system also collapsed during a particularly cold winter season. Quality controls (eg, media, serum, hormone testing) are critical for accurate and reproducible data in IVF programs (Schiewe et al, 1990) and are applied in the laboratories involved in the present study. However, despite these precautions, our data seem to indicate that conditions optimized for one culture system can be inadequate for another.…”

Quality Management Issues in the Assisted Reproduction Laboratory

“…Whittingham states that if glassware is used, it should be soaked in 7X soap solution (Linbro 7X detergent, Flow Laboratories, Rockville, MD), followed by five thorough rinses in tap water, followed by an additional three complete rinses in distilled water of culture media quality (Whittingham, 1971). Conversely, Schiewe and coworkers (Schiewe et al, 1990) asserted that after reusable instruments were soaked in 7X solution, they should be rinsed in five to seven changes of deionized water followed by three to five changes of distilled or purified water. They advocated avoidance of tap water for rinsing all items that have contact with medium or embryos because heavy metals in tap water bind to glass resolutely and can only be removed via acid washing (Schiewe et al, 1990).…”

“…Conversely, Schiewe and coworkers (Schiewe et al, 1990) asserted that after reusable instruments were soaked in 7X solution, they should be rinsed in five to seven changes of deionized water followed by three to five changes of distilled or purified water. They advocated avoidance of tap water for rinsing all items that have contact with medium or embryos because heavy metals in tap water bind to glass resolutely and can only be removed via acid washing (Schiewe et al, 1990).…”

“…Dry heat sterilization: Dry heat can also be used to sterilize glassware and stainless steel items. Schiewe and coworkers recommend that drying ovens be set at 150 o C. Items can be considered sterile after exposure to this temperature for 1 hour (Schiewe et al, 1990).…”

One‐step Vitrification Method for Cryopreservation of Bovine Embryos Transferred Directly into Recipients

“…Vitrification as a method for quick freezing without the use of a controlled-rate freezer Massip et al 1986Massip et al ,1989Scheffen et al 1986;Rall et al 1987;Rall & Mayer 1989;Kasai et al 1990;Schiewe et al 1990 Yuswiati & Holtz 1990) presents a relatively considerable progress in bovine embryo cryopreservation. Vitrification procedures used for embryo preservation are based on report of Bui- Xuan-Nguyen et al (1984) describing the process of embryo dehydratation under laboratory conditions (at room temperature).…”