Three year results of in vitro production of bovine embryos in serum-poor bovine oviduct conditioned medium. An overview

Langendonckt, Anne Van; Vansteenbrugge, A.; Donnay, Isabelle; Soom, Ann Van; Berg, U.; Semple, E.; Grisart, Bernard; Mermillod, Pascal; Brem, Gottfried; Massip, A.; Dessy, F.

doi:10.1051/rnd:19960505

Cited by 15 publications

(7 citation statements)

References 11 publications

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“…Embryo viability was similar between monolayers of different ages, in a similar way to bovine embryos cultured in oviduct cells either in suspension or on a near-confluent monolayer (McCaffrey et al, 1991). These data corroborate previous findings reporting successful results either with frozenthawed monolayers in in vivo produced embryos (Rexroad & Powell, 1988;Ellington et al, 1990), with frozen-thawed cells in in vitro produced embryos (Katska et al, 1995) or with frozen conditioned medium in in vivo derived embryos (Van Langendonckt et al, 1996). Ability of monolayers to support embryonic development was not affected by freezing-thawing of oviduct cells.…”

Section: Discussionsupporting

confidence: 89%

Effect of embryo developmental stage and culture conditions on number and quality of ovinein vitroproduced blastocysts

García-García

Domínguez

González-Bulnes

et al. 2006

Zygote

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This study evaluated the final output and quality of in vitro produced blastocysts derived from in vivo recovered sheep embryos cultured at various early developmental stages to blastocyst. A total of 270 embryos were recovered from the oviduct, at different days of the early luteal phase, and were classified into three different developmental stages: 2- to 4-cell (n = 93); 5- to 8-cell (n = 92) and 9- to 12-cell (n = 85). The effect of culture conditions was studied, at the same time, by randomly allocating the embryos to one of four groups: three groups of culture with fresh oviduct monolayers (2, 4 and 5 days old) and a fourth group with 2-day monolayers derived from frozen-thawed oviduct cells. Two control groups were established: first, embryos cultured in semi-defined medium (n = 29) and, second, blastocysts obtained in vivo and cryopreserved (n = 43). Influence on blastocyst yield of embryo developmental stage at the start of culture was statistically significant (p < 0.001). Two- to four-cell embryos showed a significantly lower developmental rate (67.7%) than the 5- to 8-cell (83.6%; p < 0.001) and 9- to 12-cell groups (90.5%; p < 0.0001) and lower quality in terms of blastocyst cryotolerance (56.0 vs. 83.7%; p < 0.005). There were no detected effects relating to the age or handling of the monolayer on the embryo developmental rate, but the day of blastocyst appearance was different between embryos cultured on monolayers derived from fresh or frozen-thawed cells (p < 0.0001); the main influence was on the group of 9- to 12-cell embryos (p < 0.0001). Current results confirm the temporal sensitivities of sheep embryos to in vitro culture, regardless of the culture conditions.

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Section: Discussionsupporting

confidence: 89%

Effect of embryo developmental stage and culture conditions on number and quality of ovinein vitroproduced blastocysts

García-García

Domínguez

González-Bulnes

et al. 2006

Zygote

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“…This suggests that the oviduct epithelium actively participates in the production of a unique biochemical milieu able to promote the in vivo oocyte maturation. Many growth factors, receptors and binding proteins are secreted into the culture medium by these so-called 'helpers' cells ' (Van Langendonckt et al 1996, Izquierdo et al 1999 and the potential of these medium, called CM, is already known for a long time, mainly for in vitro embryo production. Ijaz et al (1994) and Zhu et al (1994) defined the CM as a complex matrix of secreted products from cells, in particular, growth factors, cytokines and glycoproteins that may influence the development of early preimplantation embryos.…”

Section: Discussionmentioning

confidence: 99%

Oviductal microvesicles and their effect on in vitro maturation of canine oocytes

et al. 2017

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The effect of conditioned medium (CM) or microvesicles (MVs), secreted by multicellular spheroids of oviductal cells, and the involvement of some microRNAs (miRNAs) were investigated in canine oocyte maturation. To generate CM, spheroids were cultured for 3 days. MVs were obtained by ultracentrifugation of CM at 100,000 and measured for size and concentration by NanoSight instrument. Cumulus-oocyte complexes (COCs) were matured at 38.5°C with 5% CO and 5% of O in synthetic oviductal fluid (SOF) in biphasic systems: for 24 h, with 5.0 μg/mL of LH and for other 48 h with 10% oestrous bitch serum. SOF was used as control (CTR) or supplemented with 10% CM or 25-50-75-100-150 × 10 MVs/mL labeled with PKH-26. Results show that multicellular aggregates secreted shedding vesicles. By fluorescence microscopy, the incorporation of labeled MVs was visible only at 72 h in oocyte cytoplasm. These MVs had a positive effect ( < 0.05) on maturation rate (MII) at the concentration of 75 and 100 × 10 MVs/mL compared to CM and CTR (20.34% and 21.82% vs 9.09% and 8.66% respectively). The concentration of 150 × 10 MVs/mL provided only 9.26% of MII. The expression of three specific miRNAs (miR-30b, miR-375 and miR-503) was studied. The lower rate of MII with the higher concentration of MVs is possibly due to the high level of miR-375. In conclusion, the oviductal MVs could be involved in cellular trafficking during oocyte maturation and their possible use could facilitate the exploitment of canine reproductive biotechnologies.

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“…Zinc might be excluded as the causative factor. However, in an earlier study it was noticed that the toxic effect of silicone oil on embryo development was most pronounced when culturing embryos in serum‐free conditioned medium, less pronounced when culturing them in serum‐containing conditioned medium, and not significant when cultured in serum‐containing coculture (Van Langendonckt et al. 1996).…”

Section: Discussionmentioning

confidence: 99%

Silicone Oil Used in Microdrop Culture can affect Bovine Embryonic Development and Freezability

Soom¹,

Mahmoudzadeh²,

Christophe³

et al. 2001

Reprod Domest Anim

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The effect of silicone and paraffin oil, which is routinely used to overlay in vitro embryo culture media, on bovine in vitro embryonic development and on their subsequent survival after one-step vitrification was investigated. In experiment 1, silicone oil batch 13 was compared with paraffin oil. Embryonic development to the eight-cell and blastocyst stage was significantly impaired by silicone oil batch 13 in comparison with paraffin oil (p < 0.0001). Normal looking blastocysts produced under both types of oil were vitrified. None of the blastocysts that were produced under silicone oil batch 13 survived the vitrification procedure whereas 59% of the blastocysts survived when they were cultured under paraffin oil both before vitrification and after warming. In experiment 2, another batch no. 7 of the same brand of silicone oil was compared with paraffin oil. No effect of the type of oil on embryonic development until the eight-cell stage could be demonstrated. However, the blastocyst formation rate was significantly lower with silicone oil batch 7 than with paraffin oil. The survival of vitrified blastocysts after warming was significantly improved when silicone batch 13 was replaced by batch 7 (0 and 41%, respectively) although it was still lower when compared with the survival of blastocysts developed under paraffin oil (53%) (p < 0.05). In further attempts to find the cause of the problem, the silicone and paraffin oils were analysed for Zn-contents (experiment 3). Zn-contents were comparable for silicone oil batch 13 (0.87 microM), silicone oil batch 7 (0.62 microM) and paraffin oil (0.73 microM). Media, conditioned by bovine oviduct epithelial cells cultured with a silicone or paraffin oil overlay were analysed by means of thin layer chromatography for differences in qualitative fatty acid composition. It was possible to detect that oil overlay changed the relative abundance of fatty acids in the different lipid classes. In the free fatty acid fraction, it was skewed in favour of palmitic acid and in cholesterol esters and phospholipids in favour of linoleic acid. It appears that due to the changed lipid contents of the medium, embryonic membranes are rendered more susceptible to freezing due to altered membrane composition or by membrane damage caused by lipid peroxidation.

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Three year results of in vitro production of bovine embryos in serum-poor bovine oviduct conditioned medium. An overview

Cited by 15 publications

References 11 publications

Effect of embryo developmental stage and culture conditions on number and quality of ovinein vitroproduced blastocysts

Effect of embryo developmental stage and culture conditions on number and quality of ovinein vitroproduced blastocysts

Oviductal microvesicles and their effect on in vitro maturation of canine oocytes

Silicone Oil Used in Microdrop Culture can affect Bovine Embryonic Development and Freezability

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