One‐step Vitrification Method for Cryopreservation of Bovine Embryos Transferred Directly into Recipients

Řı́ha, Jan

doi:10.1111/j.1439-0531.1994.tb00557.x

Reprod Domestic Animals

1994

DOI: 10.1111/j.1439-0531.1994.tb00557.x

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One‐step Vitrification Method for Cryopreservation of Bovine Embryos Transferred Directly into Recipients

Jan Řı́ha

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Cited by 4 publications

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“…Vitrification of embryos can be carried out in 0.25-mL straws (Massip et al 1986;Ishimori et al 1993;¤íha 1994;Lane et al 1998;Hurtt et al 2000;and others), in glass micropipettes (Kong et al 2000a) or in microdrops of the cryoprotectant solution (¤íha et al 1991;Yang and Leibo 1999;Papis et al 1999;.…”

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Effect of Vitrification in Open Pulled Straws on Survival of Bovine Embryos from Superovulated Cows

Lopatářová¹,

Čech²,

Havlíček³

et al. 2002

Acta Vet. Brno

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Bovine embryos ) collected from superovulated cows were exposed to open pulled straw (OPS) vitrification before in vitro culture for 72 hours. The aim of this cryopreservation study applied to embryos of different developmental stages and morphological quality grades was to assess embryo survival and process of embryo handling during commercial embryo transfer (ET) procedures using the conventional freezing method as a control.During the culture of thawed quality grade 1 embryos, the hatched blastocyst stage was reached by 38.6% (27/70) of the vitrified and by 34.5% (29/84) of the conventionally frozen (control) embryos (p > 0.05). The corresponding proportions for quality grade 2 and 3 embryos were 18.5% (10/54) vs. 5.4% (2/37) and 11.8% (8/68) vs. 2% (1/50), p > 0.05, respectively.Hatching rates of embryos vitrified or conventionally frozen at the morula stage were 35.7% (15/42) and 30.3% (17/56), p > 0.05, respectively. No significant difference was found between hatching rates of embryos vitrified or conventionally frozen at the stage of early blastocysts (29.3%; 12/41 vs. 34%; 18/54). The hatching rates of embryos vitrified or conventionally frozen at the blastocyst and expanded blastocyst stages were 30.8% (12/39) vs. 21.6% (11/51), p > 0.05 and 29.3% (11/37) vs.11.4% (5/44), p < 0.05, respectively.The study demonstrated about the same survival rates for vitrified and conventionally cryopreserved embryos of all quality grades and developmental stages during in vitro embryo culture. Expanded blastocysts survived better vitrification than conventional freezing (p < 0.05). OPS vitrification is an effective and rapid method of cryopreservation of bovine embryos.

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mentioning

confidence: 99%

Effect of Vitrification in Open Pulled Straws on Survival of Bovine Embryos from Superovulated Cows

Lopatářová¹,

Čech²,

Havlíček³

et al. 2002

Acta Vet. Brno

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An efficient and mass reproducible method for vitrifying mouse embryos on a paper in cryotubes

et al. 2013

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The effect of media, serum and temperature on in vitro survival of bovine blastocysts after Open Pulled Straw (OPS) vitrification

Vajta¹,

Rindom

Peura

et al. 1999

Theriogenology

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One‐step Vitrification Method for Cryopreservation of Bovine Embryos Transferred Directly into Recipients

Cited by 4 publications

References 23 publications

Effect of Vitrification in Open Pulled Straws on Survival of Bovine Embryos from Superovulated Cows

Effect of Vitrification in Open Pulled Straws on Survival of Bovine Embryos from Superovulated Cows

An efficient and mass reproducible method for vitrifying mouse embryos on a paper in cryotubes

The effect of media, serum and temperature on in vitro survival of bovine blastocysts after Open Pulled Straw (OPS) vitrification

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