The effect of media, serum and temperature on in vitro survival of bovine blastocysts after Open Pulled Straw (OPS) vitrification

Vajta, Gábor; Rindom, N; Peura, Teija; Holm, Preben Bach; Greve, T.; Callesen, Henrik

doi:10.1016/s0093-691x(99)00184-3

Cited by 96 publications

(62 citation statements)

References 38 publications

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“…On the other hand pregnancy rates following transfer of programmable frozen compact morulae, early blastocysts, blastocysts and expanded blastocysts regardless of quality grades were 29%, 50%, 33% and 50%, respectively. Analogous to our observations, the studies of Vajta et al (1997bVajta et al ( , 1998aVajta et al ( , 1999 and Lazar et al (2000) demonstrated that advanced stages of in vitro produced bovine embryos can be vitrified successfully with hatching rates reaching 60% to 94%. Even in vitro produced embryos vitrified at the hatched blastocyst stage reached up to 81% re-expansion rate after 24 h of culture.…”

Section: Discussionsupporting

confidence: 86%

The effect of vitrification in open pulled straws on pregnancy rates after transfer of in vivoproduced bovine embryos

Lopatářová¹,

Čech²,

Holý³

et al. 2006

Vet. Med. - Czech

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The aim of this study was to compare pregnancy rates after transfer of in vivo produced embryos cryopreserved using open pulled straw (OPS) vitrification (Group V) or conventional freezing method as a control (Group C). Bovine embryos (Day<sub>6.5–7.5</sub>) collected from superovulated cows were classified according to developmental stages and morphological qualities (Grade 1 and 2) before cryopreservation and they were transferred to synchronized heifers after thawing. Pregnancy rates after transfer of morulae, early blastocysts and expanded blastocysts in Group V compared to Group C (54.5%, 12/22 vs. 56.0%, 14/25; 53.3%, 16/30 vs. 58.1%, 18/31 and 57.7%, 15/26 vs. 48.3%, 14/29) were not different (P > 0.05). Likewise, pregnancy rates after transfer of embryos of Grade 1 and 2 in Group V compared to Group C (55.1%, 43/78 vs. 54.1%, 46/85 and 36.4%, 12/33 vs. 32.9%, 23/70, respectively) were not different (P > 0.05). The study demonstrated similar viability of embryos which were frozen by vitrification or conventional method irrespective of their quality and developmental stage after transfer into recipients.

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Section: Discussionsupporting

confidence: 86%

The effect of vitrification in open pulled straws on pregnancy rates after transfer of in vivoproduced bovine embryos

Lopatářová¹,

Čech²,

Holý³

et al. 2006

Vet. Med. - Czech

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“…Slow freezing of in vitro produced embryos reduced post thaw survival rates compared with their in vivo counterparts, mostly due to their susceptibility to ice crystal formation. Cryopreservation of embryos by vitrification yields higher survival than conventional slow freezing [14,[29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification

et al. 2008

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The aim of this study was to examine the effects of co-culture with Vero cells during the in vitro maturation (IVM) and three culture media, B2 + 5% fetal calf serum (FCS) on Vero cells, synthetic oviduct fluid (SOF) + 5% FCS, and SOF + 20 g L À1 bovine serum albumin (BSA), on the developmental competence of the embryos and their ability to survive vitrification/warming. We also tested the effect of morphological quality and the age of the embryo on its sensitivity to vitrification. The IVM system neither affects the embryo development up to Day 7 nor survival rates after vitrification. The culture of embryos in SOF + FCS and in Vero cells + B2 allowed obtaining more Day 6 and Day 7 blastocysts, and a higher % of Day 7 blastocysts vitrified than culture in SOF + BSA. Contrarily, on Day 8, more blastocysts were vitrified in SOF + BSA than in SOF + FCS. Blastocysts quality affected development after vitrification/warming, and Day 7 embryos showed higher survival rates than their Day 8 counterparts. Day 7 blastocysts produced in Vero cells or in SOF + BSA survived at higher rates than those produced in SOF + FCS at 24 and 48 h after warming. Embryo culture with BSA allows obtaining hatching rates after vitrification/warming higher than those obtained after coculture with Vero cells in B2 and FCS. Moreover, this system provides hatching rates from Day 8 blastocysts comparable to those obtained on Day 7 in Vero cells. Further studies, including embryo transfer to recipients, are needed to clarify factors affecting the freezability of in vitro produced bovine embryos. #

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“…In this regard, a number of different embryo culture protocols have been proposed in order to maximize post-warming survival of vitrified embryos. Most of these studies have been mainly focused on the possible effects of serum, glucose, NADPH (nicotinamide adenosine dinucleotide phosphate), BSA (bovine serum albumin) and co-culturing on the freezability of IVP embryos [2,3,9,10]. However, little attention has been paid to the contributing effects of antioxidants on cryosurvival of the vitrified/warmed embryos.…”

mentioning

confidence: 99%

Antioxidant supplementation of culture medium during embryo development and/or after vitrification-warming; which is the most important?

Hosseini

Forouzanfar

Hajian

et al. 2009

J Assist Reprod Genet

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Purpose To determine the most optimal stage for antioxidant supplementation of culture medium to improve developmental competence, cryotolerance and DNAfragmentation of bovine embryos. Methods Presumptive zygotes were first cultured in presence or absence of β-mercaptoethanol (β-ME), for 8 days. Subsequently, half of the expanded blastocysts developed in both groups were vitrified, warmed within 30 min and post-warming embryos along with their corresponding non-vitrified embryos were cultured for two further days in presence or absence of (100 µM) βME. Results For vitrified and non-vitrified embryos, the best effect was found when βME was added from day 1 of in vitro culture in continuation with post-warming culture period. Day 1-8 supplementation significantly increased the rates of cleavage, day 7 and day 8 blastocyst production. For non-vitrified embryos, βME addition during day 1-8 and/or 9-10 of embryo culture improved both hatching rate and quality of hatched embryos. For vitrified embryos, however, the percentage of DNA-fragmentation (18.5%) was significantly higher (p≤0.05) than that of embryos developed in absence of βME but supplemented with βME during post-warming period (13.5%). Conclusions Exogenous antioxidant increases the chance of embryos, even those of fair-quality, to develop to blastocyst. However, antioxidant inclusion during in vitro embryo development is not sufficient to maintain the redox state of these embryos during the critical period of post-warming embryo culture, and therefore, there should be a surplus source of exogenous antioxidant during post-warming embryo culture.

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The effect of media, serum and temperature on in vitro survival of bovine blastocysts after Open Pulled Straw (OPS) vitrification

Cited by 96 publications

References 38 publications

The effect of vitrification in open pulled straws on pregnancy rates after transfer of in vivoproduced bovine embryos

The effect of vitrification in open pulled straws on pregnancy rates after transfer of in vivoproduced bovine embryos

Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification

Antioxidant supplementation of culture medium during embryo development and/or after vitrification-warming; which is the most important?

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