Qualitative and quantitative analysis of glucosinolates and nucleosides in Radix Isatidis by HPLC and liquid chromatography tandem mass spectrometry

Abstract

Multi-component fingerprinting and quantitation of the glucosinolates and nucleosides in samples of Radix Isatidis have been carried out using high-performance liquid chromatography with diode-array detection and electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI/MS). Five nucleosides together with one glucosinolate were identified by comparing retention times, ultraviolet spectra, mass spectra and/or empirical molecular formulae of reference compounds. Quantitation of these six compounds was carried out simultaneously by HPLC on a Phenomenex Luna C18 column using gradient elution with methanol and water and detection at 254 nm. All calibration curves were linear (r40.9994) within test ranges. Limits of detection and quantitation were 0.33 ng and 2.50 ng on column, respectively. Intra-and inter-day precision (as relative standard deviation) for all analytes was o2.19% with recoveries in the range 99.6%-101.8% at three concentration levels. The validated method was successfully applied to fingerprinting and assay of 25 batches of Radix Isatidis sourced from different geographical regions of China. The method is simple and reliable and has potential value in the quality control of Radix Isatidis.

Integrating qualitative and quantitative characterization of traditional Chinese medicine injection by high-performance liquid chromatography with diode array detection and tandem mass spectrometry

“…A reference fingerprint of DHI was generated on the basis of the chromatogram fingerprints of all samples using the software of the similarity evaluation system and the peak from Salvianolic acid B was used as reference. And then the similarity of the chromatogram from a sample with the reference fingerprint was assessed by calculating the correlation coefficient and/or angle cosine value of the original data . As shown in Fig.…”

Ban-Lan-Gen Granule Alleviates Dextran Sulfate Sodium-Induced Chronic Relapsing Colitis in Mice via Regulating Gut Microbiota and Restoring Gut SCFA Derived-GLP-1 Production

“…9,10 Additionally, several soluble compounds including glucosinolates (R,S-goitrin, progoitrin, epiprogoitrin and gluconapin) and nucleosides (hypoxanthine, adenosine, uridine and guanosine) and indigoid alkaloids (eg, indigo and indirubin) have been isolated and identified from the aqueous extract of Radix Isatidis. 11,12 Previous studies have well documented that the compounds adenosine, uridine, and indirubin exhibit potent anti-colitis effects in different animal colitis models. [13][14][15][16][17] However, no evidence-based study has been conducted to assess the efficacy of BLG against colitis.…”

Effects of elevated ultraviolet radiation on primary metabolites in selected alpine algae and cyanobacteria

“…The most significant changes were noticed concerning highly polar compounds, which showed to be nucleosides and aromatic amino acids. Till date only a few analytical studies described the determination of nucleosides in medicinal plants [17,18] . However, to the best of our knowledge this study is the first report on the quantification of these metabolites in algae and cyanobacteria.…”