Integrating qualitative and quantitative characterization of traditional Chinese medicine injection by high-performance liquid chromatography with diode array detection and tandem mass spectrometry

Abstract

The present study aims to describe and exemplify an integrated strategy of the combination of qualitative and quantitative characterization of a multicomponent mixture for the quality control of traditional Chinese medicine injections with the example of Danhong injection (DHI). The standardized chemical profile of DHI has been established based on liquid chromatography with diode array detection. High-performance liquid chromatography coupled with time-of-flight mass spectrometry and high-performance liquid chromatography with electrospray multistage tandem ion-trap mass spectrometry have been developed to identify the major constituents in DHI. The structures of 26 compounds including nucleotides, phenolic acids, and flavonoid glycosides were identified or tentatively characterized. Meanwhile, the simultaneous determination of seven marker constituents, including uridine, adenosine, danshensu, protocatechuic aldehyde, p-coumaric acid, rosmarinic acid, and salvianolic acid B, in DHI was performed by multiwavelength detection based on high-performance liquid chromatography with diode array detection. The integrated qualitative and quantitative characterization strategy provided an effective and reliable pattern for the comprehensive and systematic characterization of the complex traditional Chinese medicine system.

Comparative Efficacy of Danshen Class Injections for Treating Acute Coronary Syndrome: A Multidimensional Bayesian Network Meta-Analysis of Randomized Controlled Trials

“…Since DH launch in 2002, it has been widely used to prevent and treat ACS ( Yao et al, 2011 ). The main active ingredients of DH are flavonoids and phenolic compounds, for example, danshensu, salvianolic acid B, protocatechuic aldehyde, tanshinone IIA, rosmarinic acid and hydroxysafflor yellow A ( Xie et al, 2014 ; Wang et al, 2020 ). In addition, previous pharmacological studies have demonstrated that DH have the effects of antioxidant, anti-inflammatory, vasodilation and protect cardiac muscle due to its main pharmacological active components ( Wan et al, 2011 ; Jiang et al, 2015 ).…”

Quantitative Analysis and Comparison of Flavonoids in Lotus Plumules of Four Representative Lotus Cultivars

“…The calibration curves of four standards all showed good linearity at the concentration range (r 2 = 1 00). The limit of detection (LOD) and the limit of quantification (LOQ) were calculated based on the signal-to-noise ratios as 3 and 10, respectively [19,20]. The regression equation, LOD and LOQ of four standards (i.e., isoschaftoside, schaftoside, rutin, and isoquercitrin) were shown in Table 1.…”

Discrimination of crude and processed rhubarb products using a chemometric approach based on ultra fast liquid chromatography with ion trap/time-of-flight mass spectrometry

“…To data LC-MS with reasonable qualitative and quantitative capability has been increasingly used for chemical profiling and QC of TCMs [6,10]. Among various LC platforms, ultra fast liquid chromatography (UFLC)…”