Quadrivalent mosaic HexaPro-bearing nanoparticle vaccine protects against infection of SARS-CoV-2 variants

Kang, Yinfeng; Sun, Cong; Sun, Jing; Xie, Chu; Zhuang, Zhen; Xu, Hui-Qin; Liu, Zheng; Liu, Yihao; Peng, Sui; Yuan, Runyu; Zhao, Jincun; Zeng, Mu‐Sheng

doi:10.1038/s41467-022-30222-w

Cited by 35 publications

(27 citation statements)

References 60 publications

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“…In another study, mice immunized with 0.5 or 0.1 μg of SARS-CoV2-2 RBD displayed on SpyCatcher-mi3 nanoparticles induced high titers of neutralizing antibodies that were superior to convalescent sera [ 65 ], demonstrating the neutralizing potency of nanoparticle vaccines even at low doses. Mosaic nanoparticle vaccine candidates co-displaying mutant SARS-CoV-2 spikes have also been developed that induced neutralization of heterologous strains in mice and cynomolgus macaques [ 30 , 66 ]. However, many factors, such as nanoparticle-based antigen safety, longterm stability, and efficacy, need to be thoroughly investigated before advancement into clinical trials.…”

Section: Discussionmentioning

confidence: 99%

“…These findings demonstrate the display efficiency and safety of the encapsulin–SpyTag platform developed by us for antigen presentation. The usage of SpyTag–SpyCatcher system for antigen conjugation to the nanoparticle allows for facile simultaneous display of multiple antigens, as has been previously explored in the case of I53 and mi3 nanoparticles for co-display of spike protein from SARS-CoV-2 variants or zoonotic coronaviruses [ 30 , 66 ]. However, for manufacturing, the necessity of a conjugation step and the presence of unligated material present potential complications.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Neutralizing Efficacy of Encapsulin Nanoparticles against SARS-CoV2 Variants of Concern

Khaleeq

Sengupta

Kumar

et al. 2023

Viruses

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Rapid emergence of the SARS-CoV-2 variants has dampened the protective efficacy of existing authorized vaccines. Nanoparticle platforms offer a means to improve vaccine immunogenicity by presenting multiple copies of desired antigens in a repetitive manner which closely mimics natural infection. We have applied nanoparticle display combined with the SpyTag–SpyCatcher system to design encapsulin–mRBD, a nanoparticle vaccine displaying 180 copies of the monomeric SARS-CoV-2 spike receptor-binding domain (RBD). Here we show that encapsulin–mRBD is strongly antigenic and thermotolerant for long durations. After two immunizations, squalene-in-water emulsion (SWE)-adjuvanted encapsulin–mRBD in mice induces potent and comparable neutralizing antibody titers of 105 against wild-type (B.1), alpha, beta, and delta variants of concern. Sera also neutralizes the recent Omicron with appreciable neutralization titers, and significant neutralization is observed even after a single immunization.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Neutralizing Efficacy of Encapsulin Nanoparticles against SARS-CoV2 Variants of Concern

Khaleeq

Sengupta

Kumar

et al. 2023

Viruses

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“…Zeng et al . [ 377 ] developed a quadrivalent mosaic nanoparticle vaccine that combined four spike proteins, including SARS-CoV-2 prototype and three different variants against COVID-19 infection.…”

Section: Biomaterials Applied In Public Health Emergencymentioning

confidence: 99%

Recent advances in regenerative biomaterials

Cao

Ding

2022

Regenerative Biomaterials

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Nowadays, biomaterials have evolved from the inert supports or functional substitutes, to the bioactive materials able to trigger or promote the regenerative potential of tissues. The interdisciplinary progress has broadened the definition of “biomaterials”, and a typical new insight is the concept of tissue induction biomaterials. The term “regenerative biomaterials” and thus the contents of the present paper are relevant to yet beyond tissue induction biomaterials. This review summarizes the recent progress of medical materials including metals, ceramics, hydrogels, other polymers, and bio-derived materials. As the application aspects are concerned, this paper introduces regenerative biomaterials for bone and cartilage regeneration, cardiovascular repair, three dimensional bioprinting, wound healing, and medical cosmetology. Cell-biomaterial interactions are highlighted. Since the global pandemic of COVID-19, the review particularly mentions biomaterials for public health emergency. In the last section, perspectives are suggested: (1) Creation of new materials is the source of innovation; (2) Modification of existing materials is an effective strategy for performance improvement; (3) Biomaterial degradation and tissue regeneration are required to be harmonious with each other; (4) Host responses can significantly influence the clinical outcomes; (5) The long-term outcomes should be paid more attention to; (6) The non-invasive approaches for monitoring in vivo dynamic evolution are required to be developed; (7) Public health emergencies call for more research & development of biomaterials; (8) Clinical translation needs to be pushed forward in a full-chain way. In the future, more new insights are expected to be shed into the brilliant field — regenerative biomaterials.

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“…The pathogenesis following SARS-CoV-2 infection may comprise acute respiratory distress syndrome and deadly pneumonia, also related to the cytokine storm that induces secondary tissue impairment ( 2 ). Despite the availability of COVID-19 vaccines, clinical evidence shows that emerging mutations result in unexpected escape from vaccine effect ( 3 ). The protection of existing vaccines may be affected over time against the highly infectious Omicron variant ( 4 ).…”

Section: Introductionmentioning

confidence: 99%

Preclinical findings: The pharmacological targets and molecular mechanisms of ferulic acid treatment for COVID-19 and osteosarcoma via targeting autophagy

Pang

Yi²,

Luo³

et al. 2022

Front. Endocrinol.

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The variant virus-based 2019 coronavirus disease (COVID-19) pandemic has reportedly impacted almost all populations globally, characterized by a huge number of infected individuals. Clinical evidence proves that patients with cancer are more easily infected with severe acute respiratory disease coronavirus 2 (SARS-CoV-2) because of immunologic deficiency. Thus, there is an urgent need to develop candidate medications to treat patients with cancer plus COVID-19, including those with osteosarcoma (OS). Ferulic acid, a latent theriacal compound that has anti-tumor and antivirus activities, is discovered to have potential pharmacological use. Thus, in this study, we aimed to screen and determine the potential therapeutic targets of ferulic acid in treating patients with OS plus COVID-19 as well as the pharmacological mechanisms. We applied a well-established integrated methodology, including network pharmacology and molecular docking technique, to detail target prediction, network construction, gene ontology, and pathway enrichment in core targets. The network pharmacology results show that all candidate genes, by targeting autophagy, were the core targets of ferulic acid in treating OS and COVID-19. Through molecular docking analysis, the signal transducer and activator of transcription 3 (STAT3), mitogen-activated protein kinase 1 (MAPK1), and phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) were identified as the pharmacological targets of ferulic acid in treating OS. These preclinical findings from bioinformatics analysis altogether effectively determined the pharmacological molecules and mechanisms via targeting autophagy, demonstrating the therapeutic effectiveness of ferulic acid against COVID-19 and OS.

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Quadrivalent mosaic HexaPro-bearing nanoparticle vaccine protects against infection of SARS-CoV-2 variants

Cited by 35 publications

References 60 publications

Neutralizing Efficacy of Encapsulin Nanoparticles against SARS-CoV2 Variants of Concern

Neutralizing Efficacy of Encapsulin Nanoparticles against SARS-CoV2 Variants of Concern

Recent advances in regenerative biomaterials

Preclinical findings: The pharmacological targets and molecular mechanisms of ferulic acid treatment for COVID-19 and osteosarcoma via targeting autophagy

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