Recent advances in regenerative biomaterials

Cao, Dinglingge; Ding, Jiandong

doi:10.1093/rb/rbac098

Cited by 99 publications

(48 citation statements)

References 496 publications

(412 reference statements)

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“…Cell–material interactions are critical for tissue regeneration. − Cells respond to various material cues, cell cues and soluble factors. − As material cues are concerned, surface topography and matrix stiffness are two physical factors which always exist more or less. The present study distinguishes itself as the focus understanding of a part of topological effect in light of overall stiffness effect.…”

Section: Discussionmentioning

confidence: 99%

Stereo Coverage and Overall Stiffness of Biomaterial Arrays Underly Parts of Topography Effects on Cell Adhesion

Wang

Liu

Gao

et al. 2023

ACS Appl. Mater. Interfaces

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Surface topography is a biophysical factor affecting cell behaviors, yet the underlying cues are still not clear. Herein, we hypothesized that stereo coverage and overall stiffness of biomaterial arrays on the scale of single cells underly parts of topography effects on cell adhesion. We fabricated a series of microarrays (micropillar, micropit, and microtube) of poly(L-lactic acid) (PLLA) using mold casting based on pre-designed templates. The characteristic sizes of array units were less than that of a single cell, and thus, each cell could sense the micropatterns with varied roughness. With human umbilical vein endothelial cells (HUVECs) as the model cell type, we examined spreading areas and cell viabilities on different surfaces. "Stereo coverage" was defined to quantify the actual cell spreading fraction on a topographic surface. Particularly in the case of high micropillars, cells were confirmed not able to touch the bottom and had to partially hang among the micropillars. Then, in our opinion, a cell sensed the overall stiffness combining the bulk stiffness of the raw material and the stiffness of the culture medium. Spreading area and single cell viability were correlated to coverage and topographic feature of the prepared microarrays in particular with the significantly protruded geometry feather. Cell traction forces exerted on micropillars were also discussed. These findings provide new insights into the surface modifications toward biomedical implants.

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Section: Discussionmentioning

confidence: 99%

Stereo Coverage and Overall Stiffness of Biomaterial Arrays Underly Parts of Topography Effects on Cell Adhesion

Wang

Liu

Gao

et al. 2023

ACS Appl. Mater. Interfaces

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“…To date, many kinds of materials have been employed to fabricate artificial bone, including metals (titanium alloy), bioactive ceramics (HA, tricalcium phosphate, bioactive glass), natural (collagen, chitosan) and synthetic (PCL, PLGA, polylactic acid) polymers, and composite materials. 54 For the metals and bioactive ceramics, they possess high mechanical strength to meet the requirements of mechanical properties of segmental bone defect, while the manufacturing (high temperature and long time) for artificial bone is harsh and tissue inflammation resulting from the wear particulates often occurs. 55 The natural polymers have good biocompatibility and excellent hydrophilicity, which is beneficial for cell adhesion and proliferation; however, their poor mechanical property is far from the needs of bone implants.…”

Section: Preparation and Characterization Of Compositementioning

confidence: 99%

Flowerbed-Inspired Biomimetic Scaffold with Rapid Internal Tissue Infiltration and Vascularization Capacity for Bone Repair

et al. 2023

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The favorable microstructure and bioactivity of tissue-engineered bone scaffolds are closely associated with the regenerative efficacy of bone defects. For the treatment of large bone defects, however, most of them fail to meet requirements such as adequate mechanical strength, highly porous structure, and excellent angiogenic and osteogenic activities. Herein, inspired by the characteristics of a “flowerbed”, we construct a short nanofiber aggregates-enriched dual-factor delivery scaffold via 3D printing and electrospinning techniques for guiding vascularized bone regeneration. By the assembly of short nanofibers containing dimethyloxalylglycine (DMOG)-loaded mesoporous silica nanoparticles with a 3D printed strontium-contained hydroxyapatite/polycaprolactone (SrHA@PCL) scaffold, an adjustable porous structure can be easily realized by changing the density of nanofibers, while strong compressive strength will be acquired due to the framework role of SrHA@PCL. Owing to the different degradation performance between electrospun nanofibers and 3D printed microfilaments, a sequential release behavior of DMOG and Sr ions is achieved. Both in vivo and in vitro results demonstrate that the dual-factor delivery scaffold has excellent biocompatibility, significantly promotes angiogenesis and osteogenesis by stimulating endothelial cells and osteoblasts, and effectively accelerates tissue ingrowth and vascularized bone regeneration through activating the hypoxia inducible factor-1α pathway and immunoregulatory effect. Overall, this study has provided a promising strategy for constructing a bone microenvironment-matched biomimetic scaffold for bone regeneration.

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“…The last decade has witnessed rapid progress in biomaterials, − among which, biodegradable hydrogels have made a significant contribution. , Amphiphilic block copolymers composed of poly(ethylene glycol) (PEG) and biodegradable polyesters such as poly( d , l -lactide- co -glycolide) (PLGA) or poly( d , l -lactide) (PLA) could exhibit sol–gel transition with an increase of temperature in water under appropriate molecular parameters. Owing to good biocompatibility, controllable thermogelation, appropriate lasting time in vivo, and other excellent properties, PEG/polyester injectable thermogels show great potential in many applications, , such as drug delivery, tissue engineering, prevention of postoperative adhesion, and so on. − The hydrophilic PEG block has reversed temperature sensitivity, whereas the polyester block is hydrophobic and degradable by the hydrolysis of ester bonds .…”

Section: Introductionmentioning

confidence: 99%

Degradation-Influenced/Induced Self-Assembly of Copolymers with the Combinatory Effects of Changed Molecular Weight and Dispersity

Wei

Cui

et al. 2023

Macromolecules

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Biodegradable polymers constitute an important class of new materials, in particular biomedical materials. While degradation has been studied extensively as a "destroy" factor of a material for many years, it is less investigated when treated as a "construction" factor to influence a material in a dynamic manner. Herein, we examined a hydrolyzable amphiphilic block copolymer and found significant reorganization of the condensed state of copolymers during degradation in water. The reorganization occurs, depending upon polymer composition and experimental condition, both mesoscopically and macroscopically, which we term as "degradation-influenced/induced self-assembly (DISA)". To this end, we developed dynamic Monte Carlo simulations by introduction of hydrolysis probability while keeping the chain microrelaxation modes. A series of dynamic Monte Carlo simulations of amphiphilic triblock copolymers was carried out in a selective solvent, and three types of DISA were revealed: micelle 1 to micelle 2, sol−gel 1 to sol−gel 2, and precipitate to sol−gel−precipitate. Subsequently, amphiphilic block copolymers poly(D,L-lactide)-bpoly(ethylene glycol)-b-poly(D,L-lactide) (PLA-PEG-PLA) were synthesized, and all of the three DISA types were confirmed in experiments of the aqueous systems of three copolymers of different block lengths. We found that the DISA was regulated by both the decrease of molecular weight (MW) and the increase of dispersity. Here the term "dispersity" emphasizes not only the conventional molecular weight distribution (MWD) of all species but also the more diversified components after degradation, which include blends of copolymers and the degradation-generated homopolymers and oligomers. We also employed fluorescence resonance energy transfer to confirm the percolated network of semibald micelles underlying the temperature-induced physical hydrogel in such systems. The present study illustrates that degradation can act as a new strategy to influence the hierarchical selfassembly of amphiphilic block copolymer chains.

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Recent advances in regenerative biomaterials

Cited by 99 publications

References 496 publications

Stereo Coverage and Overall Stiffness of Biomaterial Arrays Underly Parts of Topography Effects on Cell Adhesion

Stereo Coverage and Overall Stiffness of Biomaterial Arrays Underly Parts of Topography Effects on Cell Adhesion

Flowerbed-Inspired Biomimetic Scaffold with Rapid Internal Tissue Infiltration and Vascularization Capacity for Bone Repair

Degradation-Influenced/Induced Self-Assembly of Copolymers with the Combinatory Effects of Changed Molecular Weight and Dispersity

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