Pyriproxyfen induces intracellular calcium overload and alters antioxidant defenses in Danio rerio testis that may influence ongoing spermatogenesis

Oliveira, Vanessa Staldoni de; Castro, Allisson Jhonatan Gomes; Marins, Katiuska; Mendes, Ana Karla Bittencourt; Leite, Gabriel Adan Araújo; Zamoner, Ariane; Kraak, Glen Van Der; Silva, Fátima Regina Mena Barreto

doi:10.1016/j.envpol.2020.116055

Cited by 18 publications

(14 citation statements)

References 39 publications

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“…ROS are naturally formed in an aerobic environment and, at physiological levels, are suggestive of oxidative eustress, or low-level oxidative stress [ 54 ]; however, ROS formed under pathological conditions and resulting in increased ROS indicates oxidative distress [ 55 ]. Enzymatic and nonenzymatic antioxidants are important for maintaining the oxidative eustress balance and redox status and provide a defense against ROS formation [ 54 , 56 ]. Pesticides/herbicides are known to induce ROS and lead to oxidative stress in fish [ 57 – 60 ].…”

Section: Discussionmentioning

confidence: 99%

[Retracted] Acetochlor Affects Bighead Carp (Aristichthys Nobilis) by Producing Oxidative Stress, Lowering Tissue Proteins, and Inducing Genotoxicity

Mahmood

Hussain

Ghaffar

et al. 2022

BioMed Research International

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Acetochlor is persistently used in the agroproduction sector to control broadleaf weeds. Due to frequent and continuous applications, this herbicide can reach nearby water bodies and may induce deleterious changes in aquatic life. Therefore, investigation of harmful impacts of different environmental pollutants, including herbicides, is vital to knowing the mechanisms of toxicity and devising control strategies. The current experiment included bighead carp ( n = 80 ) to estimate adverse impacts. Fish were randomly placed in 4 different experimental groups (T0-T3) and were treated for 36 days with acetochlor at 0, 300, 400, and 500 μg/L. Fresh blood without any anticoagulant was obtained and processed for nuclear and morphological changes in erythrocytes. At the same time, various visceral organs, including the gills, liver, brain, and kidneys, were removed and processed on days 12, 24, and 36 to determine oxidative stress and various antioxidant biomarkers. Comet assays revealed significantly increased DNA damage in isolated cells of the liver, kidneys, brain, and gills of treated fish. We recorded increased morphological and nuclear changes ( P ≤ 0.05 ) in the erythrocyte of treated fish. The results on oxidative stress showed a higher quantity of oxidative biomarkers and a significantly ( P ≤ 0.05 ) low concentration of cellular proteins in the gills, liver, brain, and kidneys of treated fish compared to unexposed fish. Our research findings concluded that acetochlor renders oxidative stress in bighead carp.

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Section: Discussionmentioning

confidence: 99%

[Retracted] Acetochlor Affects Bighead Carp (Aristichthys Nobilis) by Producing Oxidative Stress, Lowering Tissue Proteins, and Inducing Genotoxicity

Mahmood

Hussain

Ghaffar

et al. 2022

BioMed Research International

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“…In its reduced form, GSH is capable of scavenging ROS (Couto et al, 2016). Corroborating the results of the present study using zebrafish ovaries, we previously demonstrated that exposure to PPF for 14 days increased GST activity and decreased GSH content in the testis of adult male zebrafish (Staldoni de Oliveira et al, 2021).…”

Section: Discussionsupporting

confidence: 88%

“…Fish received 0.0001% DMSO as vehicle (control group) or were exposed to PPF from a stock solution of 10 −3 M (diluted in 100% DMSO). PPF was added to the aquarium for 7 days at a final concentration 10 −9 M (Staldoni de Oliveira et al, 2021). The water was changed every day and fresh DMSO or PPF were added.…”

Section: Methodsmentioning

confidence: 99%

“…In zebrafish, PPF causes an imbalance in the estradiol/testosterone ratio, along with the reduction in number of mature spermatozoa and an induction of vitellogenesis in males, whereas in females it inhibits vitellogenesis and reduces the number of vitellogenic oocytes (Maharajan et al, 2020). PPF has been previously reported to induce toxicity in non‐target invertebrates as insect and vertebrates, such as zebrafish (Ko et al, 2017; Staldoni de Oliveira et al, 2021). Moreover, some studies have demonstrated that PPF impairs the female and insect male genital systems (Maharajan et al, 2020; Ono et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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In vivo exposure to pyriproxyfen causes ovarian oxidative stress and inhibits follicle maturation in zebrafish

Oliveira

Marins

Mendes

et al. 2023

J of Applied Toxicology

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We analyzed the effects of pyriproxyfen (PPF) on oxidative stress and ovarian morphology in zebrafish. PPF (10−9 M) exposure increased reactive oxygen species generation in ovaries, in association with a decrease in glutathione content. The activities of glutathione S‐transferase, superoxide dismutase, and catalase were increased, while γ‐glutamyltransferase activity was not altered by pesticide treatment. The histology of ovarian tissue showed an increase in the number of previtellogenic oocytes I, and a decrease in the rate of vitellogenic oocyte (VIT) count, suggesting inhibition of follicular maturation. An increase in the thickness of the vitelline envelope was observed in VIT, as was a tendency toward an increase in atresia in the ovary of the PPF‐treated group. These findings indicate that the deleterious effect of PPF on ovarian maturation is mediated by a redox imbalance and oxidative damage. So, PPF acts as an endocrine disruptor chemical and may compromise fish reproduction by reducing female fertility.

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“…Recent studies have shown that an imbalance between the antioxidant enzyme defense system, ROS, proteolytic enzymes, and oxidants is the main cause of periodontal tissue damage [ 41 ]. Under normal conditions, intracellular antioxidant enzymes and antioxidants can neutralize ROS produced during cell metabolism; however, excessive ROS weakens the antioxidant capacity of SOD and CAT, thus increasing the consumption of intracellular SOD, CAT, and the non-enzymatic antioxidant GSH, making cells vulnerable to attack by oxidants and resulting in tissue damage [ 42 , 43 , 44 ]. GSH, SOD, and CAT, as the main antioxidant enzymes [ 45 ], can clear accumulated ROS and reduce cell and tissue damage caused by oxidative stress [ 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

Deuterohemin-Ala-His-Thr-Val-Glu-Lys (DhHP-6) Mimicking Enzyme as Synergistic Antioxidant and Anti-Inflammatory Material for Periodontitis Therapy

et al. 2022

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Periodontitis is an inflammatory disease induced by plaque microorganisms. In the clinic, antibiotic assistant periodontal mechanical therapy is the most effective therapy for the treatment of periodontitis. However, the drug resistance of the antibiotics and the repeated coming and diminishing of the disorder of oxidation–reduction balance in the inflammatory tissue could not meet the high requirements for periodontic health control in long periods. Deuterohemin-ala-his-thr-val-glu-lys (DhHP-6) is a biomimetic oxidase-mimicking enzyme that simulates the reactive oxygen radical scavenger function of heme by synthesizing the new molecular material following the key structure and amino acid sequence of heme. In this article, we report the antioxidant and anti-inflammatory properties of DhHP-6 by building a inflammatory model for human gingival fibroblasts (HGFs) stimulated by lipolysaccharide (LPS) and its effects on periodontitis in Wistar rats. DhHP-6 reduced the oxidative stress of HGFs by increasing the amount of the reductase species of glutathione (GSH) and catalase (CAT) while decreasing the amount of oxidase species of malonaldehyde (MDA) and reactive oxygen species (ROS). DhHP-6 had a dose-dependent protective effect on alveolar bone absorption in rats with periodontitis, enhanced antioxidant capacity, and reduced inflammation. As determined by Micro-CT scanning, DhHP-6 reduced alveolar bone loss and improved the bone structure of the left maxillary first molar of rats. There were no obvious morphological and histological differences in the rat organs with or without DhHP-6 treatment. These results suggest that DhHP-6 can be used to treat periodontitis by increasing the expression levels of antioxidant enzymes and antioxidants in systemic and local tissues, thereby reducing levels of oxidation products and cyto-inflammatory factors. The synergistic antioxidant and anti-inflammatory effects of DhHP-6 suggest that there are promising applications of this biomimetic enzyme molecular material for the next generation of agents for periodontitis therapy.

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Pyriproxyfen induces intracellular calcium overload and alters antioxidant defenses in Danio rerio testis that may influence ongoing spermatogenesis

Cited by 18 publications

References 39 publications

[Retracted] Acetochlor Affects Bighead Carp (Aristichthys Nobilis) by Producing Oxidative Stress, Lowering Tissue Proteins, and Inducing Genotoxicity

[Retracted] Acetochlor Affects Bighead Carp (Aristichthys Nobilis) by Producing Oxidative Stress, Lowering Tissue Proteins, and Inducing Genotoxicity

In vivo exposure to pyriproxyfen causes ovarian oxidative stress and inhibits follicle maturation in zebrafish

Deuterohemin-Ala-His-Thr-Val-Glu-Lys (DhHP-6) Mimicking Enzyme as Synergistic Antioxidant and Anti-Inflammatory Material for Periodontitis Therapy

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