Pyrimidine 5’Nucleotidase I and II Activities in Acute Leukaemias

Abstract

Bacground and objective: Pyrimidine 5’nucleotidase I and II activities of peripheral mononuclear cells were studied to evaluate their role in diagnosis, assessment of therapy and follow up of remission in acute leukaemias. Design and methods: Blood samples were obtained from 40 untreated patients with acute lymphoblastic and myeloid leukaemia and 40 healthy controls, before the therapy and after remission. The correlation between the activity of the enzymes and the efficacy of therapy were established. The enzyme activities were measured by High-Performance Liquid Chromatography (HPLC), using the method described by Amici. For statistical analysis, Mann-Whitney U, Kruskal-Wallis and Wilcoxon methods were used. Results: Before the therapy, Pyrimidine 5’nucleotidase I levels in the leukaemic group were found to be significantly elevated when compared to the control group (p<0.001). Also Pyrimidine 5’nucleotidase II levels were significantly elevated before the therapy and during remission (p<0.02 and p<0.001 respectively). The isoenzyme activities were compared in patients who were in remission, who did not respond to therapy and in patients who died during the therapy, but no significant difference was found. Interpretation and conclusions: We concluded that, Pyrimidine 5’nucleotidase I and II activities can be used as markers for diagnosis and follow up of remission in patients with acute leukaemia. But, they can not have predictive value for prognosis.

Anticancer effect of Limonin against benzo(a)pyrene‐induced lung carcinogenesis in Swiss albino mice and the inhibition of A549 cell proliferation through apoptotic pathway

“…5′-NTs is an enzyme that hydrolyzes nucleotides through a phosphate group on carbon atom 5 of ribos. [37] In the current study, an elevated level of all the above serum marker enzymes was observed in cancer-bearing mice and the previous study also reported the same results. [7] Treatment with limonin reverted back the levels of these serum marker enzymes to near to normal, which is suggestive of its anticancer potential.…”

“…LDH is necessary for continuous glycolysis, essential for the acceleration of tumor growth and a possible tumor marker enzyme in evaluating the proliferation of malignant cells. 5′‐NTs is an enzyme that hydrolyzes nucleotides through a phosphate group on carbon atom 5 of ribos . In the current study, an elevated level of all the above serum marker enzymes was observed in cancer‐bearing mice and the previous study also reported the same results .…”

Chemopreventive Task of Capsaicin against Benzo(a)pyrene‐induced Lung Cancer in Swiss Albino Mice

“…5′‐NTs are enzymes that hydrolyze nucleotides with a phosphate group on carbon atom 5 of ribose. It is found to be widely distributed in tumour tissues and increased activity of this enzyme in leukaemia patients had already been reported [34]. LDH is recognized as a potential tumour marker enzyme in assessing the proliferation of malignant cells, and increased lung and serum LDH activity has been reported in lung cancer [32].…”

Vanillic Acid Inhibits Lung Carcinogenesis by Modulates Glycoprotein Abnormalities, Membrane-Bound Enzymes, and Inflammatory Markers

“…5'-NT are enzymes that hydrolyze nucleotides with a phosphate group on carbon atom 5 of ribose. Increased activities of 5'-NT in tumorous animals appear to have originated from the proliferating tumor cells [41,42]. LDH is a fairly sensitive marker for solid neoplasms, also raised activity of the enzyme was designated in the serum of patients with lung cancer [43].…”