Pyridone Luciferins and Mutant Luciferases for Bioluminescence Imaging

Zhang, Brendan S.; McCutcheon, David C.; Prescher, Jennifer A.

doi:10.1002/cbic.201700542

Cited by 26 publications

(28 citation statements)

References 60 publications

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“…Replacement of the sulfur atom in the thiazoline ring of d -luciferin and AL with carbon or selenium resulted in luminescence wavelengths of 547 nm and 600 nm, respectively (Compound 47 , 48 ) [ 43 , 44 ]. Substitution of pyridone ring instead of the benzothiazole ring showed a luminescence wavelength of 570 nm or 530 nm (Compound 49 , 50 ) [ 45 ].…”

Section: Bioluminescent System With Longer Wavelengthmentioning

confidence: 99%

Molecular Design of d-Luciferin-Based Bioluminescence and 1,2-Dioxetane-Based Chemiluminescence Substrates for Altered Output Wavelength and Detecting Various Molecules

Takakura

2021

Molecules

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Optical imaging including fluorescence and luminescence is the most popular method for the in vivo imaging in mice. Luminescence imaging is considered to be superior to fluorescence imaging due to the lack of both autofluorescence and the scattering of excitation light. To date, various luciferin analogs and bioluminescence probes have been developed for deep tissue and molecular imaging. Recently, chemiluminescence probes have been developed based on a 1,2-dioxetane scaffold. In this review, the accumulated findings of numerous studies and the design strategies of bioluminescence and chemiluminescence imaging reagents are summarized.

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Section: Bioluminescent System With Longer Wavelengthmentioning

confidence: 99%

Molecular Design of d-Luciferin-Based Bioluminescence and 1,2-Dioxetane-Based Chemiluminescence Substrates for Altered Output Wavelength and Detecting Various Molecules

Takakura

2021

Molecules

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“…Furthermore, luciferase signals can be easily quantified [ 64 ]. Being widely used for in vitro analysis, the intracellular functions of these bioluminescent systems can be hindered by their overall dim signals, limited choice of wavelengths, and due to the limited availability of the luciferin substrates [ 65 ].…”

Section: Reporting Systems In Germsmentioning

confidence: 99%

Intracellular Imaging with Genetically Encoded RNA-based Molecular Sensors

et al. 2019

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Genetically encodable sensors have been widely used in the detection of intracellular molecules ranging from metal ions and metabolites to nucleic acids and proteins. These biosensors are capable of monitoring in real-time the cellular levels, locations, and cell-to-cell variations of the target compounds in living systems. Traditionally, the majority of these sensors have been developed based on fluorescent proteins. As an exciting alternative, genetically encoded RNA-based molecular sensors (GERMS) have emerged over the past few years for the intracellular imaging and detection of various biological targets. In view of their ability for the general detection of a wide range of target analytes, and the modular and simple design principle, GERMS are becoming a popular choice for intracellular analysis. In this review, we summarize different design principles of GERMS based on various RNA recognition modules, transducer modules, and reporting systems. Some recent advances in the application of GERMS for intracellular imaging are also discussed. With further improvement in biostability, sensitivity, and robustness, GERMS can potentially be widely used in cell biology and biotechnology.

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“…[26–28] Both blue- and red-shifted analogs have been synthesized in recent years, although most remain weak emitters with native luciferases. [26,27,29] Engineering enzymes to better process the modified analogs—and thus recover light intensity—has been successful in some cases. [27,29,30]…”

Section: Generating a Palette Of Bioluminescent Probesmentioning

confidence: 99%

“…[26,27,29] Engineering enzymes to better process the modified analogs—and thus recover light intensity—has been successful in some cases. [27,29,30]…”

Section: Generating a Palette Of Bioluminescent Probesmentioning

confidence: 99%

Advances in bioluminescence imaging: new probes from old recipes

Yao

Zhang

Prescher

2018

Current Opinion in Chemical Biology

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Bioluminescent probes are powerful tools for visualizing biology in live tissues and whole animals. Recent years have seen a surge in the number of new luciferases, luciferins, and related tools available for bioluminescence imaging. Many were crafted using classic methods of optical probe design and engineering. Here we highlight recent advances in bioluminescent tool discovery and development, along with applications of the probes in cells, tissues, and organisms. Collectively, these tools are improving in vivo imaging capabilities and bolstering new research directions.

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Pyridone Luciferins and Mutant Luciferases for Bioluminescence Imaging

Cited by 26 publications

References 60 publications

Molecular Design of d-Luciferin-Based Bioluminescence and 1,2-Dioxetane-Based Chemiluminescence Substrates for Altered Output Wavelength and Detecting Various Molecules

Molecular Design of d-Luciferin-Based Bioluminescence and 1,2-Dioxetane-Based Chemiluminescence Substrates for Altered Output Wavelength and Detecting Various Molecules

Intracellular Imaging with Genetically Encoded RNA-based Molecular Sensors

Advances in bioluminescence imaging: new probes from old recipes

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